PR3 / C-ANCA antibody

Principal name

PR3 / C-ANCA antibody

Alternative names for PR3 / C-ANCA antibody

Myeloblastin, MBN, Leukocyte proteinase 3, Proteinase 3, PRTN3, PR-3, Neutrophil proteinase 4, NP-4, P29, Wegener autoantigen, AGP7

SwissProt ID

P24158 (Human), Q61096 (Mouse), Q9UQD8 (Human)

Gene ID

5657 (PRTN3), 7155657 (aaeA)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P), Functional assay (FN)

Background of PR3 / C-ANCA antibody

PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener’s granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst.
Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin

  1. Quick-Filters:
  2. Highly Validated (1)

Items 1 to 20 of 35 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PR3 / C-ANCA antibody

Immunofluorescence: PR3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells mL-1. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides.    Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum. Rabbit Serum Hu C, F, ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

PR3 / C-ANCA (88-98) antibody

TA326729 (2µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PR3 / C-ANCA antibody

WB Suggested Anti-PRTN3 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Lung Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PR3 / C-ANCA antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PRTN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807274) Mouse IgG2b OTI5B8 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PR3 / C-ANCA antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRTN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807292)(1:150) Mouse IgG2a OTI1D10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PR3 / C-ANCA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRTN3 (RC220949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRTN3.(1:2000) Mouse IgG2b OTI2H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PR3 / C-ANCA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRTN3 (RC220949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRTN3. Mouse IgG2b OTI6A10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PR3 / C-ANCA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRTN3 (RC220949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRTN3.(1:2000) Mouse IgG2b OTI5B9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PR3 / C-ANCA antibody

Western blot analysis using PRTN3 mouse mAb against PRTN3 (aa28-256)-hIgGFc transfected HEK293 cell lysate (1). Mouse IgG2b 3B4 Aff - Purified Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse IgG1 PR3-G2 FITC Hu C, E, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse IgG1 PR3-G2 Purified Hu C, E, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse IgG1 WGM2 FITC Hu C, E, FN, P, WB
0.1 mg / €560.00
  Acris Antibodies GmbH

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse IgG1 WGM2 Purified Hu C, E, FN, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse IgG1 PR3G-2 Liquid Hu C, E, WB
0.1 mg / €580.00
  Acris Antibodies GmbH

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PR3 / C-ANCA antibody

PRTN3 MaxPab polyclonal antibody. Western Blot analysis of PRTN3 expression in human spleen. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PR3 / C-ANCA antibody

PRTN3 MaxPab rabbit polyclonal antibody. Western Blot analysis of PRTN3 expression in mouse brain. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse IgG2b 2F10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PR3 / C-ANCA antibody

PR3 / C-ANCA Mouse IgG1 PR3-G2 Hu C, E, F, WB
0.1 mg / €820.00
  Abnova Taiwan Corp.

PR3 / C-ANCA (N-term) antibody

Human Fetal Lung; WB Suggested Anti-PRTN3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Lung; PRTN3 antibody - N-terminal region (ARP61166_P050) in Human Fetal Lung cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Items 1 to 20 of 35 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn