PRAME / MAPE antibody

Principal name

PRAME / MAPE antibody

Alternative names for PRAME / MAPE antibody

OPA-interacting protein 4, OIP4, OIP-4, Melanoma antigen preferentially expressed in tumors, Preferentially expressed antigen of melanoma

SwissProt ID

P78395 (Human)

Gene ID

23532 (PRAME), 4235324 (accA)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of PRAME / MAPE antibody

PRAME/MAPE/OIP4 is a germinal tissue-specific gene that is also expressed at high levels in haematological malignancies and solid tumors. The physiological functions of PRAME in normal and tumor cells are unknown, although a role in the regulation of retinoic acid signaling has been proposed. Sequence homology and structural predictions suggest that PRAME is related to the Leucine-rich repeat (LRR) family of proteins, which have diverse functions. PRAME, or „preferentially expressed antigen in melanoma”, was originally identified as a gene encoding a HLA-A24 restricted antigenic peptide presented to autologous tumor-specific cytotoxic T lymphocytes derived from a patient with melanoma. PRAME is synonymous with MAPE (melanoma antigen preferentially expressed in tumors) and OIP4 (OPA-interacting protein 4), and its expression profile defines it as a cancer-testis antigen. Cancer-testis antigens (CTAs) are encoded by non-mutated genes expressed at high levels in germinal tissues and tumors, but which are absent from or detected at low levels in other tissues. PRAME may be somewhat different to other cancer-testis antigens in that it shows some expression in normal tissues such as ovary, adrenal, placenta and endometrium. The C-terminus of human PRAME (amino acids 453-509) was also identified to bind Neisseria gonorrhoeae opacity factors, in this case the OPA-P protein. Thus PRAME is also known as OIP4 (OPA interacting protein), although the functional implications of the interaction are unknown.

Items 1 to 20 of 22 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRAME / MAPE antibody

Detection of PRAME in MCF7 cell lysates using anti-PRAME. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRAME / MAPE antibody

WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain Rabbit IgG Purified Bov, Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRAME / MAPE antibody

WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRAME / MAPE (C-term) antibody

Western blot analysis of MAPE antibody (C-term) (AP17546PU-N) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRAME / MAPE (C-term) antibody

Western analysis using anti-human PRAME / MAPE antibody Cat.-No. AP60005PU-N. Sample was loaded in 21% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh PRAME. Rabbit IgG Purified Hu E(detection), WB
0.2 mg / €370.00
  Acris Antibodies GmbH

PRAME / MAPE (C-term) antibody

Western analysis using anti-human PRAME / MAPE antibody Cat.-No. AP60005PU-N. Sample was loaded in 21% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh PRAME. Rabbit IgG Purified Hu E(detection), WB
0.1 mg / €270.00
  Acris Antibodies GmbH

PRAME / MAPE antibody

Western Blot analysis of PRAME expression in transfected 293T cell line (H00023532-T02) by PRAME MaxPab polyclonal antibody.

Lane 1: PRAME transfected lysate(55.99 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PRAME / MAPE antibody

PRAME / MAPE Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PRAME / MAPE antibody

Western Blot analysis of PRAME expression in transfected 293T cell line (H00023532-T02) by PRAME MaxPab polyclonal antibody.

Lane 1: PRAME transfected lysate(57.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PRAME / MAPE antibody

Western blot analysis of PRAME in MCF-7 cell lysates with PRAME polyclonal antibody ( Cat# PAB12059 ). Rabbit Hu WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

PRAME / MAPE antibody

Human Brain; WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; PRAME antibody - N-terminal region (ARP55981_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PRAME / MAPE antibody

Human HepG2; WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; PRAME antibody - N-terminal region (ARP55982_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PRAME / MAPE antibody

PRAME / MAPE Rabbit Aff - Purified Hu WB
25 µl / €140.00
  Novus Biologicals Inc.

PRAME / MAPE antibody

Immunohistochemistry-Paraffin: PRAME Antibody [NBP1-85418] Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRAME / MAPE (transcript variant 4)

PRAME / MAPE Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PRAME / MAPE (N-term HIS tag, transcript variant 3)

PRAME / MAPE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

PRAME / MAPE (full length, N-term HIS tag, transcript variant 5)

PRAME / MAPE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

PRAME / MAPE

Sandwich ELISA using the recombinant human PRAME fragment Cat.-No. AR60005PU-N as standard. Human Purified > 98 % by SDS-PAGE and Silver staining E. coli
20 µg / €200.00
  Acris Antibodies GmbH

PRAME / MAPE

PRAME / MAPE Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PRAME / MAPE

PRAME / MAPE Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Items 1 to 20 of 22 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn