PRAME / MAPE antibody

Principal name

PRAME / MAPE antibody

Alternative names for PRAME / MAPE antibody

OPA-interacting protein 4, OIP4, OIP-4, Melanoma antigen preferentially expressed in tumors, Preferentially expressed antigen of melanoma

SwissProt ID

P78395 (Human)

Gene ID

23532 (PRAME)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of PRAME / MAPE antibody

PRAME/MAPE/OIP4 is a germinal tissue-specific gene that is also expressed at high levels in haematological malignancies and solid tumors. The physiological functions of PRAME in normal and tumor cells are unknown, although a role in the regulation of retinoic acid signaling has been proposed. Sequence homology and structural predictions suggest that PRAME is related to the Leucine-rich repeat (LRR) family of proteins, which have diverse functions. PRAME, or „preferentially expressed antigen in melanoma”, was originally identified as a gene encoding a HLA-A24 restricted antigenic peptide presented to autologous tumor-specific cytotoxic T lymphocytes derived from a patient with melanoma. PRAME is synonymous with MAPE (melanoma antigen preferentially expressed in tumors) and OIP4 (OPA-interacting protein 4), and its expression profile defines it as a cancer-testis antigen. Cancer-testis antigens (CTAs) are encoded by non-mutated genes expressed at high levels in germinal tissues and tumors, but which are absent from or detected at low levels in other tissues. PRAME may be somewhat different to other cancer-testis antigens in that it shows some expression in normal tissues such as ovary, adrenal, placenta and endometrium. The C-terminus of human PRAME (amino acids 453-509) was also identified to bind Neisseria gonorrhoeae opacity factors, in this case the OPA-P protein. Thus PRAME is also known as OIP4 (OPA interacting protein), although the functional implications of the interaction are unknown.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRAME / MAPE antibody

  Rabbit Polyclonal PRAME Antibody  
Detection of PRAME in MCF7 cell lysates using anti-PRAME. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRAME / MAPE antibody

  Rabbit Polyclonal Anti-PRAME Antibody  
WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain Rabbit IgG Purified Bov, Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRAME / MAPE antibody

  Rabbit Polyclonal Anti-PRAME Antibody  
WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRAME / MAPE (C-term) antibody

Western blot analysis of MAPE antibody (C-term) (AP17546PU-N) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab. Rabbit Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRAME / MAPE (C-term) antibody

Western analysis using anti-human PRAME / MAPE antibody Cat.-No. AP60005PU-N. Sample was loaded in 21% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh PRAME. Rabbit IgG Purified Hu E(detection), WB
0.2 mg / €370.00
  Acris Antibodies GmbH

PRAME / MAPE (C-term) antibody

Western analysis using anti-human PRAME / MAPE antibody Cat.-No. AP60005PU-N. Sample was loaded in 21% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); Lane 2: rh PRAME. Rabbit IgG Purified Hu E(detection), WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PRAME - human gene knockout kit via CRISPR

PRAME - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PRAME - human gene knockout kit via CRISPR

PRAME - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRAME - human gene knockout kit via CRISPR

PRAME - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRAME - human gene knockout kit via CRISPR

PRAME - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRAME / MAPE (transcript variant 4)

  Purified recombinant protein of Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 4  
PRAME / MAPE Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PRAME / MAPE (N-term HIS tag, transcript variant 3)

  Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug  
PRAME / MAPE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

PRAME / MAPE (full length, N-term HIS tag, transcript variant 5)

  Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5, full length, with N-terminal HIS tag, expressed in E. coli, 50ug  
PRAME / MAPE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

PRAME / MAPE

Sandwich ELISA using the recombinant human PRAME fragment Cat.-No. AR60005PU-N as standard. Human Purified > 98 % by SDS-PAGE and Silver staining E. coli
20 µg / €200.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PRAME (mGFP-tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PRAME (mGFP-tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PRAME (mGFP-tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, PRAME (mGFP-tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PRAME (mGFP-tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5, 200 uL, >10^7 TU/mL

Lenti ORF particles, PRAME (mGFP-tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PRAME (Myc-DDK tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PRAME (Myc-DDK tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, PRAME (Myc-DDK tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 4 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PRAME (Myc-DDK tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 4 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, PRAME (Myc-DDK tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PRAME (Myc-DDK tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

20 Item(s)

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