PRDM1 / BLIMP1 antibody

Principal name

PRDM1 / BLIMP1 antibody

Alternative names for PRDM1 / BLIMP1 antibody

BLIMP-1, PRDI-BF1, PRDI-binding factor 1, PR domain zinc finger protein 1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1

SwissProt ID

O75626 (Human), Q60636 (Mouse)

Gene ID

9641 (IKBKE), 639 (PRDM1), 12142 (Prdm1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Can (Canine), Chk (Chicken), Chimp (Chimpanzee), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)

Background of PRDM1 / BLIMP1 antibody

This 6D3 monoclol antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptiol repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds D. In B cells, Blimp-1 promotes termil differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germil center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Filly, Blimp-1 has been demonstrated to play a significant role in the termil differentiation of effector and effector memory CD8+ T cells during viral infection.

General readings

1. Lin, Y. et al. Repression of c-myc transcription by Blimp-1, an inducer of terminal B cell differentiation. Science. 276: 596-599 (1997)
2. Lin, K. et al. Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells. Mol. & Cell. Biol. 22(13): 4771-4780 (2002)
3. Angelin-Duclos, C. et al. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo. J. Immunol. 165(10): 5462-5471(2000)
4. Ohinata, Y., et al. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature. 436: 207-213 (2005) (Mouse)
1. Chang, DH. et al. The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Mech. Dev. 1179(1-2): 305-309 (2002)
2. Cattoretti, G., et al. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.
J Pathol. 206(1):76-86 (2005) (Immunohistochemistry-paraffin, immunofluorescence, human).
3. Gong, D., and Malek, T. R. (2007) Cytokine-Dependent Blimp-1 Expression in Activated T Cells Inhibits IL-2 Production, 178, 242-252.
4. Severa, M., Coccia, E. M., and Fitzgerald, K. A. (2006) Toll-like Receptor-dependent and -independent Viperin Gene Expression and Counter-regulation by PRDI-binding Factor-1/BLIMP1, 281, 26188-26195.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRDM1 / BLIMP1 antibody

Western blot analysis of PRDM1 over-expressed 293 cell line, cotransfected with PRDM1 Validated Chimera RNAi ( Cat # H00000639-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRDM1 monoclonal antibody (M01), clone 2B10 (Cat # H00000639-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG2a 2B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PRDM1 / BLIMP1 (C-term) antibody

Anti-PRDM1 (AP10057PU-N)(1µg/ml) staining of Human Brain (cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PRDM1 / BLIMP1 (C-term) antibody

Western blot analysis of Blimp-1 in A549 cell lysate with Blimp-1 antibody at (A) 0.5 and (B) 1ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 (N-term) antibody

Western blot analysis of Blimp-1 in mouse lung tissue lysate with Blimp-1 antibody at (A) 0.5 and (B) 1ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

Western Blot: Blimp-1 antibody - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using TA309794.  1881: murine pre-B cell lysate (negative control).  Photo courtesy of DA Savitsky, Columbia University. Mouse IgG1 3H2-E8 Purified Hu, Ms, Por C, E, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

WB using Anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate (lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1/BLIMP1 (lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

Immunoblotting of reduced cell lysates prepared from A20 cell line using 5 ug/mL of Anti-Human/Mouse Blimp1 Purified.  Bands were visualized using Anti-Rat IgG HRP (Left). Rat 6D3 Purified Ms P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

PRDM1 Antibody (N-term) (Cat. #TA324553) western blot analysis in HL-60 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the PRDM1 antibody detected the PRDM1 protein (arrow). Rabbit IgG Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

A20 (lane 1) and PBMC (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- BLIMP - 1 antibody, ROS195G. Proteins were visualized using a HRP goat anti-mouse antibody and a chemiluminescence detection system Mouse IgG1 ROS195G Purified Hu, Ms E, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

WB Suggested Anti-PRDM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: SH-SYSY cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

WB Suggested Anti-PRDM1 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

Western blot analysis of extracts from JK and Raji cells using Blimp-1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

TA349001 (0.3 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRDM1 / BLIMP1 antibody

Western blot analysis using PRDM1 mouse mAb against Raji (1, 2), L1210 (3) and TPH-1 (4) cell lysate. Mouse IgG1 5E7 Ascites Hu, Ms E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

PRDM1 / BLIMP1 antibody

Colon, lymphoid follicle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PRDM1 / BLIMP1 antibody

WB using Anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate (lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1/BLIMP1 (lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

PRDM1 / BLIMP1 Control Peptide

PRDM1 / BLIMP1
50 µg / €160.00
  Acris Antibodies GmbH

PRDM1 / BLIMP1 Control Peptide

PRDM1 / BLIMP1
50 µg / €160.00
  Acris Antibodies GmbH

PRDM1 / BLIMP1 antibody

PRDM1 / BLIMP1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRDM1 / BLIMP1 antibody

Western Blot analysis of PRDM1 expression in transfected 293T cell line (H00000639-T01) by PRDM1 MaxPab polyclonal antibody.

Lane 1: PRDM1 transfected lysate(86.79 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

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