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Presenilin-1 antibody

Principal name

Presenilin-1 antibody

Alternative names for Presenilin-1 antibody

Presenilin 1, PSEN1, PS1, AD3, Alzheimer Disease 3, PS-1, PSNL1, AF3, FAD, S182

SwissProt ID

P49768 (Human), P49769 (Mouse), P97887 (Rat), Q4JIM4 (Chick), Q6RH31 (Canfa), Q9XT97 (Bovin)

Gene ID

5663 (PSEN1), 19164 (Psen1), 29192

Ncbi ID

NP_000012.1, 9606, NP_000012, NP_032969.1, NM_008943, NM_019163, NM_000021, NM_007318

Available reactivities

Hu (Human), Mky (Monkey), Hst (Hamster), Ms (Mouse), Rt (Rat), Prim (Primate), Can (Canine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Presenilin-1 antibody

In Alzheimer’s disease (AD) a progressive neurodegeneration and cognitive decline occurs during the course of the disease and amylloid beta-peptide (Abeta) is believed to play a central role for the molecular pathogenesis of AD. The gamma-Secretase is a key enzyme involved in the processing of the beta-amyloid precursor protein into Abeta. Abeta accumulates in brain and forms plaques in AD brains. One of the mechanism behind neurodegenration in AD is apoptosis due to preservation of gamma secretase activity. Several proteins (presenilin1, presenilin 2, nicastrin, Aph-1 and Pen-2) interact with gamma secretase complex during apoptosis in neuronal cells to make active gamma secretase complexes in apoptotic cells. The fragments corresponding to the PS1 N-terminal fragment and the caspase-cleaved PS1 C-terminal fragment (PS1-caspCTF) were found to form active gamma-secretase complexes when co-expressed in presenilin (PS) knockout cells (1). Mutations in presenilin 1 (PS1) lead to dominant inheritance of early-onset familial Alzheimer’s disease (FAD). These mutations are known to alter the gamma-secretase cleavage of the amyloid precursor protein (APP), resulting in increased ratio of Abeta42/Abeta40 and accelerated amyloid plaque pathology and altered intracellular Ca(2+) homeostasis involving endoplasmic reticulum Ca(2+) stores (2, 3). It is suggested that Presenilin binds to phospholipase D1 through its loop region thereby recruiting golgi/transgolgi network that perturbs gamma secretase activity and Abeta formation.
Numerous mutations in the presenilins are known to cause early-onset familial Alzheimer’s disease (FAD). These mutations are known to alter the gamma-secretase cleavage of the amyloid precursor protein (APP), resulting in increased ratio of Abeta42/Abeta40 and accelerated amyloid plaque pathology in transgenic mouse models. Presenelin 1is a 467 amino acid membrane bound protein with 8 putative TMD. It is believed to be the catalytic domain of gamma secretase complex that catalyzes the intramembrane cleavage of integral membraneproteins such as Notch receptors and beta amyloid precursor protein. Other functions include intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Some regulatory proteins associated with Resenelin are Nicastrin, NTF, CTF, APH1/2and PEN2. It also interacts with other proteins such as Notch1, cadherin 1, plakophilin 4, dock 3, catenin, HERPUD1, FLNA and FLNB.

Immunohistochemistry (IHC) analyzes of Presenilin 1 antibody (Cat.-No.: AP06290PU-N) in paraffin-embedded human brain tissue.

AP06290PU-N

Western blot(WB) analysis of Presenilin 1 antibody (Cat.-No.: AP06290PU-N) in extracts from mouse heart cells.

AP06290PU-N

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE)

AP07340PU-N

Immunohistochemical staining of human brain with Rabbit anti Human presenilin-1 (AP05739PU-N)

AP05739PU-N

Western blot analysis of human brain lysate with Rabbit anti Human presenilin-1 (AP05739PU-N) at 0.5(A), 1(B) and 2(C) µg/ml

AP05739PU-N

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PSEN1 polyclonal antibody ( Cat # PAB4815 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.

PAB4815

The PSEN1 polyclonal antibody ( Cat # PAB4815 ) is used in Western blot to detect PSEN1 in mouse kidney tissue lysate ( lane 1 ) and HL-60 cell lysate ( lane 2 ) lysate.

PAB4815

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

AP13205PU-N

The anti-PSEN1 C-term Antibody is used in Western blot to detect PSEN1 in mouse kidney tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.

AP13205PU-N

AP15743PU Presenilin 1 antibody staining of Human Brain.

Immunohistochemical staining of PSEN1 on formalin fixed, paraffin embedded human brain with PSEN1 polyclonal antibody.

PAB11738

Immunohistochemistry of PSEN1 in human brain tissue with PSEN1 polyclonal antibody ( Cat # PAB12993 ) at 2.5 µg/ml.

PAB12993

Western blot analysis of PSEN1 in human brain lysate with PSEN1 polyclonal antibody ( Cat # PAB12993 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.

PAB12993

Human OVCAR3; WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate. PSEN1 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells.; PSEN1 antibody - N-terminal region (ARP44201_P050) in Human OVCAR3 cells using Western Blot

ARP44201_P050

Immunohistochemistry analysis of paraffin-embedded human brain, using PSEN1 (Phospho-Ser357) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from RAW264.7 cells treated with UV 5', using PSEN1 (Phospho-Ser357) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PSEN1 (Ab-357) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Raw264.7 cells, treated with UV 5', using PSEN1 (Ab-357) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Presenilin 1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from mouse heart cells, using Presenilin 1 Antibody. The lane on the right is treated with the synthesized peptide.

Mouse; The PSEN1 antibody (#ARP58941) works fine in 4% paraformaldehyde fixed tissue sections (30 um), they were cut on a sliding, freezing microtome. We stained it overnight 1:500 followed with a biotinylated secondary, followed by an Avidin/peroxidase tertiary antibody, final staining with metal-intensified DAB. Similarly, both LRP antibodies (#ARP58562 &ARP58563) work fine (stain appropriately) with this procedure. We are still evaluating the BACE1 antibody, it seems to work, too. -Dr. Thomas van Groen; PSEN1 antibody - middle region (ARP58941_P050) in Mouse cells using Immunohistochemistry

ARP58941_P050

Human Small intestine; Immunohistochemistry with Small intestine tissue; PSEN1 antibody - middle region (ARP58941_P050) in Human Small intestine cells using Immunohistochemistry

ARP58941_P050

Hum. Adult Placenta; Host:Rabbit. Target Name:PSEN1. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; PSEN1 antibody - middle region (ARP58941_P050) in Hum. Adult Placenta cells using Western Blot

ARP58941_P050

Human 721_B; Host:Rabbit. Target Name:PSEN1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. PSEN1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; PSEN1 antibody - middle region (ARP58941_P050) in Human 721_B cells using Western Blot

ARP58941_P050

Mouse, Rat; . Lanes: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Gene Name: PSEN1. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science.; PSEN1 antibody - middle region (ARP58941_P050) in Mouse, Rat cells using Western Blot

ARP58941_P050

Human brain; WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; PSEN1 antibody - middle region (ARP58941_P050) in Human brain cells using Western Blot

ARP58941_P050

Mouse brain; . Amount and Sample Type :. 500 ug mouse brain homogenate. Amount of IP Antibody :. 6 ug. Primary Antibody :. PSEN1. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:5000. Gene Name :. PSEN1. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; PSEN1 antibody - middle region (ARP58941_P050) in Mouse brain cells using Immunoprecipitation

ARP58941_P050

ARP58941_P050

Human Placenta (formalin-fixed, paraffin-embedded) stained with PSEN1 antibody AP22785PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22785PU-N

Western blot analysis (with AP22785PU-N) of Hela cell line lysate (35µg/lane).

AP22785PU-N

Western blot analysis of Presenilin 1 expression in HL60 (A); HeLa (B); SKNMC (C); COS7 (D) whole cell lysates.

TP316443

Western blot analysis of presenilin1 in human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

TA306424

Immunohistochemistry of Presenilin1 in human brain tissue with Presenilin1 antibody at 2.5 ug/ml.

TA306424

Immunofluorescence of Presenilin1 in Human Brain cells with Presenilin1 antibody at 20 ug/mL.

TA306424

Western blot analysis of extracts from mouse heart cells, using presenilin 1 antibody (#TA313388).The lane on the right is treated with the synthesized peptide.

TA313388

Immunohistochemical analysis of paraffin-embedded human brain tissue using presenilin 1 antibody (#TA313388).The picture on the right is treated with the synthesized peptide.

TA313388

WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: OVCAR-3 cell lysate;PSEN1 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells

TA334642

Western Blot: Presenilin 1 Antibody [TA336586] - Analysis of Human Presenilin 1 / PSDR1 in androgen-stimulated metastatic adenocarcinoma LNCaP.FGC cells with Presenilin 1 antibody at a dilution of 1:500

TA336586

Western blot analysis on RAW264.7 cell lysate using Presenilin 1 Antibody

TA347463

Human Brain stained with anti-Presenilin antibody.

TA354038

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AP22785PU-N	Presenilin-1+2 (C-term) antibody
	Rabbit			Purified	Hu, Ms	E, P, WB	50 µg / €470.00
							Acris Antibodies GmbH
+1 additional image
TA306424	Presenilin-1 antibody
	Rabbit Polyclonal Presenilin1 Antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
+2 additional images
TA313388	Presenilin-1 antibody
	Rabbit polyclonal Presenilin 1 antibody
	Rabbit	IgG		Purified	Hu	E, P, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
+1 additional image
TA334642	Presenilin-1 antibody
	Rabbit Polyclonal Anti-PSEN1 Antibody
	Rabbit	IgG		Purified	Can, GP, Hu, Ms, Por, Rb, Rt	P, WB	50 µg / €325.00
							OriGene Technologies, Inc.
TA336586	Presenilin-1 antibody
	Rabbit Polyclonal Presenilin-1 Antibody
	Rabbit			Purified	Hu	C, E, IP, P, WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
TA347463	Presenilin-1 antibody
	Rabbit Polyclonal Anti-Presenilin 1 Antibody
	Rabbit	IgG		Purified	Hu	WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
TA352046	Presenilin-1 antibody
	Rat Monoclonal anti-Presenilin 1, 21-80 (NH2 terminus) Antibody
	Rat	IgG1	24-4B5	Purified	Hu	IP, WB	200 µl / €479.00
							OriGene Technologies, Inc.
TA352047	Presenilin-1 antibody
	Mouse Monoclonal anti-Presenilin 1 (NH2 terminus) Antibody
	Mouse	IgG1	NT1	Purified	Hu	IP, WB	200 µl / €479.00
							OriGene Technologies, Inc.
TA352048	Presenilin-1 antibody
	Mouse Monoclonal anti-Presenilin 1, 260-378 (Loop domain) Antibody
	Mouse	IgG1	34-4C3	Purified	Hu	IP, WB	200 µl / €479.00
							OriGene Technologies, Inc.
TA354038	Presenilin-1 antibody
	Rabbit Polyclonal anti-Presenilin Antibody
	Rabbit			Purified	Hu	P	0.1 ml / €325.00
							OriGene Technologies, Inc.
AP05739PU-N	Presenilin-1 antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	P, WB	0.1 mg / €360.00
							Acris Antibodies GmbH
+1 additional image
AP06290PU-N	Presenilin-1 antibody
	Rabbit			Aff - Purified	Hu	P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
AP07340PU-N	Presenilin-1 antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	P, WB	50 µg / €470.00
							Acris Antibodies GmbH
AP13205PU-N	Presenilin-1 (C-term) antibody
	Rabbit	Ig		Purified	Hu, Ms	E, P, WB	0.4 ml / €370.00
							Acris Antibodies GmbH
+1 additional image
SP1373P	Presenilin-1 antibody
	Rabbit			Serum	Hu, Mky	C, IP, WB	0.1 ml / €330.00
							Acris Antibodies GmbH
SP1374P	Presenilin-1 (N-term) antibody
	Rabbit			Serum	Hst, Hu, Mky	C	0.1 ml / €340.00
							Acris Antibodies GmbH
A8404-1	Presenilin-1 pSer357 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	E, P, WB	50 µg / €230.00
							Assay Biotech
+1 additional image
A8404-2	Presenilin-1 pSer357 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €330.00
							Assay Biotech
+1 additional image
B8404-1	Presenilin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	E, P, WB	50 µg / €230.00
							Assay Biotech
+1 additional image
B8404-2	Presenilin-1 antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €330.00
							Assay Biotech
+1 additional image