PRKAA1 antibody

Principal name

PRKAA1 antibody

Alternative names for PRKAA1 antibody

AMPK1, AMPK alpha-1 chain, 5'-AMP-activated protein kinase catalytic subunit alpha-1

SwissProt ID

P45894 (Caeel), P54645 (Rat), Q09136 (Pig), Q13131 (Human), Q5EG47 (Mouse)

Gene ID

5562 (PRKAA1), 105787 (Prkaa1), 65248

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Insect, Fi (Fish), Mky (Monkey), Dros (Drosophila), Vertebrates, Ve, Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of PRKAA1 antibody

Catalytic subunit of AMP-activated protein kise (AMPK), an energy sensor protein kise that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and ictivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-sigling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt sigling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kise activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

General readings

Dev Biol. 2006 Mar 15;291(2):227-38. Epub 2006 Jan 26.
AMPK regulation of mouse oocyte meiotic resumption in vitro.
Chen J, Hudson E, Chi MM, Chang AS, Moley KH, Hardie DG, Downs SM.
Department of Biological Sciences, Marquette University, Milwaukee, WI 53201, USA.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AMPK1/AMPK2 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPK alpha-1/2 antibody (#AP02704PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

AMPK1/AMPK2 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPK alpha-1/2 antibody (#AP02704PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

AMPK1/AMPK2 antibody

Immunohistochemical analysis using AMPK1/AMPK2 antibody (Cat.-No. AP06007PU-N) in Paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AMPK1/AMPK2 antibody

Immunohistochemistry: AMPK1/AMPK2 Antibody [NB100-81783] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPK1/AMPK2 (Ab-485/491) antibody. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €380.00
  Novus Biologicals Inc.

AMPK alpha 1/2 antibody

Immunohistochemical analysis of AMPK alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AMPK alpha 1/2 antibody

Immunohistochemical analysis of AMPK alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AMPK alpha 1/2 antibody

Immunohistochemical analysis of AMPK alpha 1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AMPK alpha 1/2 pThr183/pThr172 antibody

Immunohistochemical analysis of AMPK alpha 1/2 (pT183/172) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt IP, P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AMPK alpha 1/2 pThr183/pThr172 antibody

Immunohistochemical analysis of AMPK alpha 1/2 (pT183/172) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt IP, P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AMPK alpha 1/2 pThr183/pThr172 antibody

Immunohistochemical analysis of AMPK alpha 1/2 (pT183/172) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt IP, P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

PRKAA1 antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining. Mouse IgG1 2B7 Ascites Hu, Mky, Ms, Rt E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

PRKAA1 pSer487 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using AMPK1 pSer487 Antibody (#AP02451PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PRKAA1 pSer487 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using AMPK1 pSer487 Antibody (#AP02451PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

PRKAA1 antibody

Predicted band size: 65 kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAA1 antibody

Western blot analysis of HeLa cell lysate using PRKAA1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKAA1 antibody

PRKAA1 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAA1 antibody

Western blot analysis of AMPK alpha expression in Adriamycin treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAA1 antibody

Rat Prkaa1 Antibody (N-term) (Cat. #TA328197) western blot analysis in CEM,mouse NIH/3T3 cell line and rat cerebellum tissue lysates (35ug/lane).This demonstrates the rat Prkaa1 antibody detected the rat Prkaa1 protein (arrow). Rabbit Purified Hu, Ms, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

PRKAA1 antibody

Western Blot: AMPK alpha 1 Antibody (2B7) [TA336924] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate. Mouse IgG1 2B7 Purified Hu, Ms, Prim, Rt E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAA1 pSer486 antibody

Immunohistochemistry analyis of p-AMPK1 antibody (pSer486) Cat.-No AP01523PU-N in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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