PRKAA2 antibody

Principal name

PRKAA2 antibody

Alternative names for PRKAA2 antibody

AMPK2, AMPK alpha-2 chain, 5'-AMP-activated protein kinase catalytic subunit alpha-2

SwissProt ID

P54646 (Human), Q09137 (Rat), Q28948 (Pig), Q8BRK8 (Mouse)

Gene ID

5563 (PRKAA2)

Available reactivities

Bov (Bovine), Hu (Human), Rt (Rat), Ms (Mouse), Dros (Drosophila), Vertebrates, Ve, Can (Canine), Por (Porcine), Chk (Chicken), Xen (Xenopus), Eq (Equine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of PRKAA2 antibody

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]

General readings

1. Dong Y, Zhang M, Liang B, Xie Z, Zhao Z, Asfa S, Choi HC, Zou MH, Reduction of AMP-activated protein kinase alpha2 increases endoplasmic reticulum stress and atherosclerosis in vivo. Circulation 2010 Feb 121 (6): 792-803.
PMID: 20124121

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRKAA2 antibody

Sample (30 ug of whole cell lysate). . A:NIH-3T3. 7.5% SDS PAGE. . TA308756 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAA2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between STK11 and PRKAA2 HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-PRKAA2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 1G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PRKAA2 antibody

(1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKAA2 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using PRKAA2 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAA2 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using PRKAA2 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAA2 antibody

WB Suggested Anti-PRKAA2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKAA2 (C-term) antibody

Western blot analysis of anti-AMPK alpha2 C-term Pab (AP13880PU-N) in mouse heart tissue lysate. AMPK alpha2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKAA2 (C-term) antibody

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PRKAA2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKAA2 (Center)/(Thr172) antibody

PRKAA2 (Thr172) antibody (Center) (Cat. #AP53442PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the PRKAA2 (Thr172) antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKAA2 antibody

Immunofluorescence of purified MaxPab antibody to PRKAA2 on 293 cell. [antibody concentration 1 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PRKAA2 antibody

Western Blot analysis of PRKAA2 expression in transfected 293T cell line (H00005563-T02) by PRKAA2 MaxPab polyclonal antibody.

Lane 1: PRKAA2 transfected lysate(62.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PRKAA2 antibody

Western blot analysis of PRKAA2 polyclonal antibody ( Cat # PAB2767 ) in mouse heart tissue lysate. PRKAA2 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRKAA2 pSer175 antibody

Western blot analysis of extract from A-431 cells, untreated or treated with EGF ( 200 ng/ml, 5 min ), using PRKAA2 (phospho S175) polyclonal antibody ( Cat # PAB5805 ). Rabbit Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PRKAA2 antibody

Rat; . Sample Type: rat hepatocyte (50ug). Priamry Dilution: 1:4000. Secondary Antibody: Donkey anti-Rabbit HRP. Secondary Dilution: 1:10,000. Image Submitted By: Dustin Singer,. INRA/AgroParistech .; PRKAA2 antibody - middle region (ARP56697_P050) in Rat cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

PRKAA2 antibody

Western blot analysis of AMPK alpha 2 expression in Hela (A); 293T (B); C2C12 (C); NIH3T3 (D); rat heart (E); rat brain (F) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PRKAA2 antibody

Western blot analysis of AMPK alpha 2 expression in Hela (A); 293T (B); C2C12 (C); NIH3T3 (D); rat heart (E); rat brain (F) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PRKAA2 antibody

Western blot analysis of AMPK alpha 2 expression in Hela (A); 293T (B); C2C12 (C); NIH3T3 (D); rat heart (E); rat brain (F) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

PRKAA2 antibody

Western Blot: AMPK alpha 2 Antibody [NB100-238] - Detection of Human, Rat, and Bovine AMPK alpha 2 by Western Blot. Samples: Extracts from 1. bovine aortic endothelial cells, 2. rat aortic smooth muscle cells, 3. HepG2 cells, or 4. human aortic endothelial cells. Antibody: Affinity purified rabbit anti-AMPK alpha 2 (Cat. No. NB100-238) used at 2 mug/ml. Detection: Chemiluminescence. Rabbit Aff - Purified Bov, Hu, Rt IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

PRKAA2 antibody

PRKAA2 Rabbit Agarose Bov, Hu, Rt IP
0.1 mg / €400.00
  Novus Biologicals Inc.

PRKAA2 antibody

PRKAA2 Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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