PRKACA antibody

Principal name

PRKACA antibody

Alternative names for PRKACA antibody

PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

SwissProt ID

P00517 (Bovin), P05132 (Mouse), P17612 (Human), P27791 (Rat), P36887 (Pig)

Gene ID

5566 (PRKACA), 282322, 18747 (Prkaca)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep), Hst (Hamster), Can (Canine), Eq (Equine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot)

Background of PRKACA antibody

Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. The second messenger cAMP is produced in response to a wide variety of hormones & neurotransmitters and regulates cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters & gene transcription. The principle intracellular target for cAMP is PKA. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. Two major PKA isozymes, type I (PKAI) and type II (PKAII) consist of RI and RII respectively, complexed with C. Three different C subunits (alpha, beta, and gamma) and four different R subunits (RI-alpha, RI-beta, RII-alpha, and RII-beta) have been identified. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PKA C alpha/beta antibody

Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PKA C alpha/beta antibody

Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PKA C alpha/beta antibody

Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

PKA C alpha/beta/gamma antibody

Immunohistochemical analysis of PKA C alpha/beta/gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PKA C alpha/beta/gamma antibody

Immunohistochemical analysis of PKA C alpha/beta/gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PKA C alpha/beta/gamma antibody

Immunohistochemical analysis of PKA C alpha/beta/gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

PRKACA antibody

Sample (30 ug of whole cell lysate). A: NIH-3T3. B: C2C12. 10% SDS PAGE. TA308159 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKACA antibody

Western blot analysis of extracts from mouse brain cells, using PKA CAT (Ab-197) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKACA antibody

Western blot analysis of extracts from Jurkat cells and COLO cells, using KAPC A/B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKACA antibody

WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle Rabbit IgG Purified Eq, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKACA antibody

WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta Rabbit IgG Purified Eq, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKACA antibody

Western blot analysis on Jurkat and K562 cell lysate using KAPC A/B Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKACA antibody

Dot Blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (SM3155P). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. 
Lane 1: GST-Akt1
Lane 2: GST-Akt2
Lane 3: GST-Akt3
Lane 4: GST-PKAc alpha
Lane 5: GST-PKAc beta
Lane 6: GST-PKAc gamma
Lane 7: GST-MEK 1
Lane 8: GST Mouse IgG1 6D2.1 Aff - Purified Hu WB
0.1 mg / €230.00
  Acris Antibodies GmbH

PRKACA control peptide

PRKACA Purified
50 µg / €190.00
  Acris Antibodies GmbH

PRKACA antibody

Western blot analysis of PKA expression in lysates from Jurkat cells (Lane 1 & 2), mouse small intestine (Lane 3) and rat kidney (Lane 4). Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt, Sh IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PRKACA antibody

Western blot (WB) analysis of PKAalpha/beta cat antibody (Cat.-No.: AP01230PU-N)  in extracts from COLO cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PRKACA (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKACA (N-term) antibody

Western blot analysis of PRKACA Antibody (N-term K82) (AP17667PU-N) in NCI-H460 cell line lysates (35ug/lane). PRKACA (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKACA antibody

PRKACA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRKACA antibody

Western Blot analysis of PRKACA expression in transfected 293T cell line (H00005566-T02) by PRKACA MaxPab polyclonal antibody.

Lane 1: PRKACA transfected lysate(40.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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