PRKAR1A antibody

Principal name

PRKAR1A antibody

Alternative names for PRKAR1A antibody

PKR1, PRKAR1, TSE1, CAR, CNC1, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1

SwissProt ID

P00514 (Bovin), P07802 (Pig), P09456 (Rat), P10644 (Human), Q5ZM91 (Chick), Q9DBC7 (Mouse)

Gene ID

5573 (PRKAR1A), 19084 (Prkar1a), 25725

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Sh (Sheep), Can (Canine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PRKAR1A antibody

The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kise (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation. Ictive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this ictive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-a, C-ß, and C-?) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, a and ß (RI-a, RI-ß, RII-a, and RII-ß). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue. Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3a Ser21 and GSK-3ß Ser9). In addition, combined knock-down of PKA C-a and -ß blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRKAR1A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50) Mouse IgG1 OTI6C7 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PRKAR1A antibody

Western blot analysis of extracts of various cell lines using PRKAR1A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Western blot analysis of extracts from HepG2 cells, using KAP0 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Predicted band size: 43 kDa . Positive control: HeLa, CEM, A549, SW480, PC3, V251, HCTnb and heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HeLa cell lysate Lane2:CEM cell lysate Lane3: A549 cell lysate Lane4: SW480 cell lysate Lane5: PC3 cell lysate Lane6: V251 cell lysate Lane7: HCTnb cell lysate Lane8: Heart tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

WB Suggested Anti-PRKAR1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver Rabbit IgG Purified Can, GP, Hu, Ms, Por, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

WB Suggested Anti-Prkar1a Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Spleen Rabbit IgG Purified Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Western blot (WB) analysis of PKR1 antibody (Cat.-No.: AP01335PU-N) in extracts from COLO cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PRKAR1A (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKAR1A antibody

PRKAR1A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Western Blot analysis of PRKAR1A expression in transfected 293T cell line (H00005573-T02) by PRKAR1A MaxPab polyclonal antibody.

Lane 1: PRKAR1A transfected lysate(41.91 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Western Blot analysis of PRKAR1A expression in transfected 293T cell line (H00005573-T01) by PRKAR1A MaxPab polyclonal antibody.

Lane 1: PRKAR1A transfected lysate(43.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAR1A polyclonal antibody ( Cat # PAB3313 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Western blot analysis of extracts from HepG2 cells, using KAP0 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

PRKAR1A antibody

Western blot analysis of extracts from HepG2 cells, using KAP0 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

PRKAR1A (C-term) antibody

PRKAR1A Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PRKAR1A antibody

Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PRKAR1A antibody

Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PRKAR1A antibody

Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRKAR1A (transcript variant 1)

PRKAR1A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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