PRKAR1A antibody

Principal name

PRKAR1A antibody

Alternative names for PRKAR1A antibody

PKR1, PRKAR1, TSE1, CAR, CNC1, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1

SwissProt ID

P00514 (Bovin), P07802 (Pig), P09456 (Rat), P10644 (Human), Q5ZM91 (Chick), Q9DBC7 (Mouse)

Gene ID

5573 (PRKAR1A)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Sh (Sheep), Can (Canine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of PRKAR1A antibody

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. Transcript Variant: This variant (3) represents the longest transcript. It appears to be less frequently expressed based on the EST data. All three variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRKAR1A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500641, Dilution 1:50) Mouse IgG1 OTI6C7 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PRKAR1A antibody

Western blot analysis of extracts of various cell lines using PRKAR1A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferus ducts.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Dilution 1:50) Mouse 6C7 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

PRKAR1A antibody

Western blot analysis of extracts from HepG2 cells, using KAP0 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Predicted band size: 43 kDa . Positive control: HeLa, CEM, A549, SW480, PC3, V251, HCTnb and heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HeLa cell lysate Lane2:CEM cell lysate Lane3: A549 cell lysate Lane4: SW480 cell lysate Lane5: PC3 cell lysate Lane6: V251 cell lysate Lane7: HCTnb cell lysate Lane8: Heart tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

WB Suggested Anti-PRKAR1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver Rabbit IgG Purified Can, GP, Hu, Ms, Por, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

WB Suggested Anti-Prkar1a Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Spleen Rabbit IgG Purified Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKAR1A antibody

Western blot (WB) analysis of PKR1 antibody (Cat.-No.: AP01335PU-N) in extracts from COLO cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PRKAR1A (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKAR1A antibody

PRKAR1A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Western Blot analysis of PRKAR1A expression in transfected 293T cell line (H00005573-T02) by PRKAR1A MaxPab polyclonal antibody.

Lane 1: PRKAR1A transfected lysate(41.91 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Western Blot analysis of PRKAR1A expression in transfected 293T cell line (H00005573-T01) by PRKAR1A MaxPab polyclonal antibody.

Lane 1: PRKAR1A transfected lysate(43.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAR1A polyclonal antibody ( Cat # PAB3313 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRKAR1A antibody

Western blot analysis of extracts from HepG2 cells, using KAP0 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

PRKAR1A antibody

Western blot analysis of extracts from HepG2 cells, using KAP0 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

PRKAR1A (C-term) antibody

PRKAR1A Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PRKAR1A antibody

Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PRKAR1A antibody

Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PRKAR1A antibody

Immunofluorescent analysis of PRKAR1A staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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