PRKCA antibody

Principal name

PRKCA antibody

Alternative names for PRKCA antibody

Protein kinase C alpha type, PKC-alpha, PKC alpha, PKC-A, PKCA, PRKACA

SwissProt ID

P04409 (Bovin), P05696 (Rat), P10102 (Rabit), P17252 (Human), P20444 (Mouse)

Gene ID

5578 (PRKCA), 282001

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mam (Mammalia), Rb (Rabbit), Bov (Bovine), Can (Canine), Mky (Monkey), Fi (Fish), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of PRKCA antibody

Activation of protein kise C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction . PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation at Thr500 in the activation loop, the autophosphorylation site at Thr641, and at carboxy-termil hydrophobic site Ser660 occurs in vivo. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Either the enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCµ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kises (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kise activity.

General readings

General / background references:Van der Zee EA et al. Differential distribution of protein kinase C (PKC alpha beta and PKC gamma) isoenzyme immunoreactivity in the chick brain. Brain Res 676:41-52 (1995). PubMed PMID: 7796177
Ferro TJ et al. Tumor necrosis factor-alpha activates pulmonary artery endothelial protein kinase C. Am J Physiol 264:L7-14 (1993). PubMed PMID: 8430818
Young S et al. A monoclonal antibody recognizing the site of limited proteolysis of protein kinase C. Inhibition of down-regulation in vivo. Eur J Biochem 173:247-52 (1988). PubMed PMID: 2451608

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PKC (pan) antibody

Immunohistochemistry analysis of pan PKC Antibody (Cat.-No AP06566PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PKC alpha/beta/gamma antibody

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-PKC monoclonal antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG2a PKC0103 Aff - Purified Bov, Hu, Ms, Rt IP, WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

PKC alpha/beta/gamma antibody

PKC alpha/beta/gamma Mouse IgG2a MC5 Liquid Bov, Hu, Ms, Rt IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PRKCA antibody

Western blot analysis of extracts of Jurkat and K562 cells, using PKC¦Á antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

Immunoperoxidase of monoclonal antibody to PRKCA on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml] Mouse IgG 2F11 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PRKCA antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308938 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

Western blot analysis of extracts from MCF-7 cells and Jurkat cells, using PKC a (Ab-657) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

Predicted band size: 77kDa. Positive control: Jurkat and 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 293T cell lysate Lysates: 50 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

Predicted band size: 77kDa. Positive control: Jurkat and HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HT-29 cell lysate Lysates: 50 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

Western blot analysis of PKC alpha expression in UV treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

Western blot analysis of PKC-pan expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [TA336677] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain. Mouse IgG2a MC5 Purified Bov, Can, Fi, Hu, Ms, Por, Rt C, E, F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

PRKCA Rabbit G13-R Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 ml / €460.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCA antibody

PRKCA Rabbit G13-R Aff - Purified Hu, Ms, Rt E, IP, WB
50 µl / €310.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCA antibody

PRKCA Rabbit IgG D22-G FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCA antibody

Immunohistochemistry (IHC) analyzes of PKC alpha antibody (Cat.-No.: AP06563PU-N) in paraffin-embedded human breast Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PRKCA pThr497 (491-501) antibody

Figure 1. Staining PRKCA in Spleen by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

PRKCA (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKCA (N-term) antibody

Western blot analysis of PRKCA Antibody (N-term) (AP18158PU-N) in NIH-3T3 cell line lysates (35ug/lane). PRKCA (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKCA antibody

Western blot (WB) analysis of PKC alpha antibody (Cat.-No.: AP21092PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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