PRKCA antibody

Principal name

PRKCA antibody

Alternative names for PRKCA antibody

Protein kinase C alpha type, PKC-alpha, PKC alpha, PKC-A, PKCA, PRKACA

SwissProt ID

P04409 (Bovin), P05696 (Rat), P10102 (Rabit), P17252 (Human), P20444 (Mouse)

Gene ID

5578 (PRKCA), 18750 (Prkca)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit), Mam (Mammalia), Can (Canine), Fi (Fish), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)

Background of PRKCA antibody

Activation of protein kise C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction . PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation at Thr500 in the activation loop, the autophosphorylation site at Thr641, and at carboxy-termil hydrophobic site Ser660 occurs in vivo. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Either the enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCµ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kises (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kise activity.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PKC (pan) antibody

Immunohistochemistry analysis of pan PKC Antibody (Cat.-No AP06566PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PKC alpha/beta/gamma antibody

  Mouse Monoclonal anti-PKC Antibody  
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-PKC monoclonal antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG2a PKC0103 Aff - Purified Bov, Hu, Ms, Rt IP, WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit anti-PRKCA Polyclonal Antibody  
Western blot analysis of extracts of Jurkat and K562 cells, using PKC¦Á antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit Polyclonal antibody to PKC alpha (protein kinase C, alpha)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308938 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit polyclonal PKC a (Ab-657) antibody  
Western blot analysis of extracts from MCF-7 cells and Jurkat cells, using PKC a (Ab-657) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit polyclonal PKC a (Tyr657) antibody(Phospho-specific)  
Western blot analysis of extracts from COLO205 cells, using PKC a (Phospho-Tyr657) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Anti-PRKCA rabbit polyclonal antibody  
Predicted band size: 77kDa. Positive control: Jurkat and 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 293T cell lysate Lysates: 50 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Anti-PRKCA rabbit polyclonal antibody  
Predicted band size: 77kDa. Positive control: Jurkat and HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: HT-29 cell lysate Lysates: 50 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit Polyclonal Phospho-PKC alpha (Thr638) Antibody (Phospho-specific)  
Western blot analysis of PKC alpha phosphorylation expression in UV treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit Polyclonal Phospho-PKC-pan (Thr497) Antibody (Phospho-specific)  
Western blot analysis of PKC-pan phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit Polyclonal PKC alpha Antibody  
Western blot analysis of PKC alpha expression in UV treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit Polyclonal PKC-pan Antibody  
Western blot analysis of PKC-pan expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Mouse Monoclonal PKC alpha Antibody (MC5)  
Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [TA336677] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain. Mouse IgG2a MC5 Purified Bov, Can, Fi, Hu, Ms, Por, Rt C, E, F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Rabbit Polyclonal Anti-PRKCA Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Jurkat cells, NIH/3T3 cells, Primary antibody: (PRKCA Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

  Mouse monoclonal Anti-PKCa Clone MC5  
PRKCA Mouse IgG1 MC5 Purified Bov E, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCA antibody

PRKCA Rabbit G13-R Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 ml / €460.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCA antibody

PRKCA Rabbit G13-R Aff - Purified Hu, Ms, Rt E, IP, WB
50 µl / €310.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCA antibody

PRKCA Rabbit IgG D22-G FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCA antibody

Immunohistochemistry (IHC) analyzes of PKC alpha antibody (Cat.-No.: AP06563PU-N) in paraffin-embedded human breast Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PRKCA pThr497 (491-501) antibody

Figure 1. Staining PRKCA in Spleen by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

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