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PRKCD antibody

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Principal name

PRKCD antibody

Alternative names for PRKCD antibody

PKC-delta, PKC delta, PKCD, Protein kinase C delta type

SwissProt ID

P09215 (Rat), P28867 (Mouse), Q05655 (Human), Q5PU49 (Canfa)

Gene ID

5580 (PRKCD), 170538 (Prkcd), 18753

Ncbi ID

9606, NP_006245.2, 10116, NP_006245, NM_006254, NM_006254.3, NP_997704, NP_035233.1, BC051416, NM_011103, NM_133307, NM_212539

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of PRKCD antibody

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+. On the other hand, nPKC delta and epsilon, as well as all of the cPKC members, possess phorbol esterbinding activities and kinase activities.

H00005580-M01 - PRKCD
Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.
H00005580-M02 - PRKCD
Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.
H00005580-M02 - PRKCD
Western blot analysis of PRKCD over-expressed 293 cell line, cotransfected with PRKCD Validated Chimera RNAi ( Cat # H00005580-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCD monoclonal antibody (M02) clone 6A2 (Cat # H00005580-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00005580-M02 - PRKCD
Western Blot analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M02), clone 6A2.Lane 1: PRKCD transfected lysate(77.5 KDa).Lane 2: Non-transfected lysate.
H00005580-M03 - PRKCD
Detection limit for recombinant GST tagged PRKCD is approximately 0.03ng/ml as a capture antibody.
H00005580-M03 - PRKCD
PRKCD monoclonal antibody (M03), clone 8H6 Western Blot analysis of PRKCD expression in K-562 ( Cat # L009V1 ).
H00005580-M06 - PRKCD
Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.
H00005580-M06 - PRKCD
Immunofluorescence of monoclonal antibody to PRKCD on A-431 cell. [antibody concentration 10 ug/ml]
H00005580-M06 - PRKCD
PRKCD monoclonal antibody (M06), clone 2E12 Western Blot analysis of PRKCD expression in A-431 ( Cat # L015V1 ).
H00005580-M07 - PRKCD
Detection limit for recombinant GST tagged PRKCD is approximately 1ng/ml as a capture antibody.
H00005580-M07 - PRKCD
Western Blot analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M07), clone 8E12.Lane 1: PRKCD transfected lysate(77.5 KDa).Lane 2: Non-transfected lysate.
AP02533PU-S - PRKCD
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Ser645 PKC-delta antibody Cat.-No. AP02533PU (Left) or the same antibody preincubated with blocking peptide (Right).
AP02533PU-S - PRKCD
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using Phospho-Ser645 PKC-delta antibody Cat.-No. AP02533PU
AP02781PU-S - PRKCD
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC-delta antibody AP02781PU-N
AP02781PU-S - PRKCD
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using PKC-delta antibody AP02781PU-N (Red).
AP02781PU-S - PRKCD
Figure 3. Western blot analysis of extracts from PC12 cells using PKC-delta antibody AP02781PU-N
H00005580-B01 - PRKCD
Western Blot analysis of PRKCD expression in transfected 293T cell line (H00005580-T01) by PRKCD MaxPab polyclonal antibody.Lane 1: PRKCD transfected lysate(74.36 KDa).Lane 2: Non-transfected lysate.
AP06564PU-N - PRKCD
Western blot (WB) analysis of PKC δ antibody (Cat.-No.: AP06564PU-N)in extracts from MCF7 cells.
AP06564PU-N - PRKCD
Immunohistochemistry (IHC) analyzes of PKC δ antibody (Cat.-No.: AP06564PU-N) in paraffin-embedded human brast carcinoma tissue.
PAB4536 - PRKCD
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCD polyclonal antibody ( Cat # PAB4536 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
PAB4536 - PRKCD
Western blot analysis of PRKCD ( arrow ) using rabbit PRKCD polyclonal antibody ( Cat # PAB4536 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PRKCD gene ( Lane 2 ) ( Origene Technologies ) .
PAB4536 - PRKCD
Western blot analysis of PRKCD polyclonal antibody ( Cat # PAB4536 ) in placenta lysate . PRKCD delta ( Arrow ) was detected using purified PRKCD polyclonal antibody ( Cat # PAB4536 ) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
PAB5520 - PRKCD
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PRKCD polyclonal antibody ( Cat # PAB5520 ) .
PAB5520 - PRKCD
Western blot analysis of extract from MCF-7 cells using PRKCD polyclonal antibody ( Cat # PAB5520 ) .
H00005580-D01P - PRKCD
Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCD and FYN. HeLa cells were stained with anti-PRKCD rabbit purified polyclonal 1:1200 and anti-FYN mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00005580-D01P - PRKCD
Western Blot analysis of PRKCD expression in transfected 293T cell line (H00005580-T01) by PRKCD MaxPab polyclonal antibody.Lane 1: PRKCD transfected lysate(77.50 KDa).Lane 2: Non-transfected lysate.
AP20759PU-N - PRKCD
Immunohistochemistry (IHC) analyzes of PKC delta antibody (Cat.-No.: AP20759PU-N) in paraffin-embedded human breast carcinoma tissue.
AP20759PU-N - PRKCD
Western blot (WB) analyzes of PKC δ antibody (Cat.-No.: AP20759PU-N) in extracts from Jurkat cells.
A0717-1 - PRKCD
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC delta (Phospho-Thr505) Antibody. The picture on the right is treated with the synthesized peptide.
A0717-1 - PRKCD
Western blot analysis of extracts from NIH-3T3 cells treated with UV 15', using PKC delta (Phospho-Thr505) Antibody. The lane on the right is treated with the synthesized peptide.
A0718-1 - PRKCD
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC delta (Phospho-Tyr313) Antibody. The picture on the right is treated with the synthesized peptide.
A0718-1 - PRKCD
Western blot analysis of extracts from HepG2 cells, COLO205 cells and HuvEc cells, using PKC delta (Phospho-Tyr313) Antibody. The lane on the right is treated with the synthesized peptide.
A0801-1 - PRKCD
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is treated with the synthesized peptide.
A0801-1 - PRKCD
Immunofluorescence analysis of NIH-3T3 cells, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is treated with the synthesized peptide.
A0801-1 - PRKCD
Western blot analysis of extracts from Jurkat cells treated with starved 24h, using PKC delta (Phospho-Tyr52) Antibody. The lane on the right is treated with the synthesized peptide.
A7196-1 - PRKCD
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC delta (Phospho-Ser645) Antibody. The picture on the right is treated with the synthesized peptide.
A7196-1 - PRKCD
Western blot analysis of extracts from COS7 cells and A549 cells, using PKC delta (Phospho-Ser645) Antibody. The lane on the right is treated with the synthesized peptide.
A8171-1 - PRKCD
Western blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30', using PKCD (Phospho-Tyr64) Antibody. The lane on the right is treated with the synthesized peptide.
B0717-1 - PRKCD
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PKC delta (Ab-505) Antibody. The picture on the right is treated with the synthesized peptide.
B0717-1 - PRKCD
Western blot analysis of extracts from NIH-3T3 cells, treated with UV 15', using PKC delta (Ab-505) Antibody. The lane on the right is treated with the synthesized peptide.
B0801-1 - PRKCD
Immunofluorescence analysis of HeLa cells, using PKC delta (Ab-52) Antibody. The picture on the right is treated with the synthesized peptide.
B7196-1 - PRKCD
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKC delta (Ab-645) Antibody. The picture on the right is treated with the synthesized peptide.
B7196-1 - PRKCD
Western blot analysis of extracts from MCF7 cells, using PKC delta (Ab-645) Antibody. The lane on the right is treated with the synthesized peptide.
B8171-1 - PRKCD
Western blot analysis of extracts from NIH-3T3 cells, treated with EGF 200ng/ml 30', using PKCD (Ab-64) Antibody. The lane on the right is treated with the synthesized peptide.
AP55877PU-S - PRKCD
Western blot analysis of extracts from HepG2 cells treated with PMA using Phospho-PKCD (Tyr64) antibody AP55877PU-N.The lane on the right is treated with the antigen-specific peptide.
CPA1934-100ul - PRKCD
Immunohistochemical analysis of PKC delta (pT507) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA1934-100ul - PRKCD
Western blot analysis of PKC delta (pT507) expression in HeLa H2O2-treated (A); A549 H2O2-treated (B); Raw264.7 H2O2-treated (C); PC12 H2O2-treated (D) whole cell lysates.
CPA1935-100ul - PRKCD
Western blot analysis of PKC delta (pY52) expression in MCF7 serum starvation-treated (A); HeLa PMA-treated (B); Jurkat (C); Raw264.7 serum starvation-treated (D); H9C2 serum starvation-treated (E) whole cell lysates.
CPA3295-100ul - PRKCD
Immunohistochemical analysis of PKC delta (pY313) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3295-100ul - PRKCD
Western blot analysis of PKC delta (pY313) expression in HeLa (A); MCF7 Caylculin A-treated (B) whole cell lysates.
TP308127 - PRKCD
TA312515 - PRKCD
Western blot analysis of extracts from MCF7 cells, using PKC delta (Ab-645) Antibody.The lane on the right is treated with the synthesized peptide.
TA312515 - PRKCD
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKC delta (Ab-645) Antibody.The picture on the right is treated with the synthesized peptide.
TA312516 - PRKCD
Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using PKCD (Ab-64) antibody.The lane on the right is treated with the synthesized peptide.
TA312518 - PRKCD
Immunofluorescence analysis of HeLa cells, using PKC d antibody.The picture on the right is treated with the synthesized peptide.
TA324893 - PRKCD
Western blot analysis of PKC delta (arrow) using rabbit polyclonal PKC delta Antibody (N-term) (Cat. #TA324893). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2) (Origene Technologies).
TA324893 - PRKCD
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
TA327882 - PRKCD
Western blot analysis of PKC delta expression in MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA327883 - PRKCD
Western blot analysis of PKC delta expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA327884 - PRKCD
Western blot analysis of PKCD expression in EGF treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA349581 - PRKCD
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (PRKCD Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
TA349581 - PRKCD
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (PRKCD Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA349581 - PRKCD
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (PRKCD Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA312521 - PRKCD
Western blot analysis of extracts from COS7 cells and A549 cells, using PKCd (phospho-Ser645) antibody.The lane on the right is treated with the synthesized peptide.
TA312521 - PRKCD
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PKCd (phospho-Ser645) antibody.The picture on the right is treated with the synthesized peptide.
TA312520 - PRKCD
Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using PKCD (Phospho-Tyr64) antibody.The lane on the right is treated with the synthesized peptide.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRKCD antibody
  Rabbit polyclonal PKCd (Ab-645) antibody  
Western blot analysis of extracts from MCF7 cells, using PKC delta (Ab-645) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

PRKCD antibody
  Rabbit polyclonal PKCD (Ab-64) antibody  
Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using PKCD (Ab-64) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCD antibody
  Rabbit polyclonal anti-PKC d antibody  
Immunofluorescence analysis of HeLa cells, using PKC d antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCD antibody
  Rabbit polyclonal PKCD (Tyr64) antibody(Phospho-specific)  
Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using PKCD (Phospho-Tyr64) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCD antibody
  Rabbit polyclonal PKCd (Ser645) antibody(Phospho-specific)  
Western blot analysis of extracts from COS7 cells and A549 cells, using PKCd (phospho-Ser645) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

PRKCD (N-term) antibody
  Rabbit polyclonal PKC delta Antibody (N-term)  
Western blot analysis of PKC delta (arrow) using rabbit polyclonal PKC delta Antibody (N-term) (Cat. #TA324893). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

PRKCD antibody
  Rabbit Polyclonal PKC delta Antibody  
Western blot analysis of PKC delta expression in MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCD antibody
  Rabbit Polyclonal PKC delta Antibody  
Western blot analysis of PKC delta expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCD antibody
  Rabbit Polyclonal PKCD Antibody  
Western blot analysis of PKCD expression in EGF treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCD antibody
  Rabbit Polyclonal Anti-PRKCD Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (PRKCD Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

PRKCD antibody
PRKCD Rabbit K15-K Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 ml / €460.00
  DB Biotech, spol. s r.o.
 

Sample sizes available

PRKCD antibody
PRKCD Rabbit K15-K Aff - Purified Hu, Ms, Rt E, IP, WB
50 µl / €310.00
  DB Biotech, spol. s r.o.
 

Sample sizes available

PRKCD antibody
PRKCD Rabbit IgG R20-F FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.
 

Sample sizes available

PRKCD pSer645 antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Ser645 PKC-delta antibody Cat.-No. AP02533PU (Left) or the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH
+1 additional image

PRKCD pSer645 antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Ser645 PKC-delta antibody Cat.-No. AP02533PU (Left) or the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH
+1 additional image

PRKCD antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC-delta antibody AP02781PU-N Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH
+2 additional images

PRKCD antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKC-delta antibody AP02781PU-N Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH
+2 additional images

PRKCD antibody
Western blot (WB) analysis of PKC δ antibody (Cat.-No.: AP06564PU-N)in extracts from MCF7 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

PRKCD antibody
Immunohistochemistry (IHC) analyzes of PKC delta antibody (Cat.-No.: AP20759PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

PRKCD pTyr64 antibody
Western blot analysis of extracts from HepG2 cells treated with PMA using Phospho-PKCD (Tyr64) antibody AP55877PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

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