PRKCE antibody

Principal name

PRKCE antibody

Alternative names for PRKCE antibody

PKC-epsilon, PKC epsilon, PKCE, Protein kinase C epsilon type

SwissProt ID

P09216 (Rat), P10830 (Rabit), P16054 (Mouse), Q02156 (Human)

Gene ID

5581 (PRKCE), 18754 (Prkce), 29340, 1055816 (Q8CRC1)

Available reactivities

Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Luminex (LMNX)

Background of PRKCE antibody

Activation of protein kise C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-termil hydrophobic site Ser660. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCµ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kises (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kise activity.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRKCE antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502395) Mouse IgG1 OTI1B7 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502415) Mouse IgG2b OTI4G8 Purified Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502417) Mouse IgG2b OTI1B4 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502437) Mouse IgG2b OTI2A12 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

Anti-PRKCE mouse monoclonal antibody (TA502438) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE(RC217702). Mouse IgG1 OTI2E9 Purified Can, Hu, Mky F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502439) Mouse IgG2b OTI4B3 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502540) Mouse IgG1 OTI3A12 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

PRKCE ELISA with 1B7 Capture (TA600071) and 1B4 Detection (TA700070) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI1B7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCE antibody

PRKCE ELISA with 1G5 Capture (TA600072) and 1B7 Detection (TA700071) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI1G5 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCE antibody

PRKCE ELISA with 3F10 Capture (TA600074) and 1B7 Detection (TA700071) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI3F10 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCE antibody

PRKCE ELISA with 4G8 Capture (TA600076) and 1B4 Detection (TA700070) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI4G8 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCE antibody

PRKCE ELISA with 4G8 Capture (TA600076) and 1B4 Detection (TA700070) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI1B4 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

PRKCE antibody

PRKCE ELISA with 1G5 Capture (TA600072) and 1B7 Detection (TA700071) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI1B7 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

PRKCE antibody

PRKCE ELISA with 4G8 Capture (TA600076) and 2A12 Detection (TA700072) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI2A12 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

PRKCE antibody

PRKCE ELISA with 1G5 Capture (TA600072) and 4G8 Detection (TA700073) Antibodies. Substrate used: Recombinant Human PRKCE (TP317702) Mouse IgG1 OTI4G8 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

PRKCE antibody

Anti-PKC-e (DB 010). Western blot of PKC-e in mouse brain crude lysate (50μg of protein loaded). Rabbit G13-X Aff - Purified Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €450.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCE antibody

Anti-PKC-e (DB 010). Western blot of PKC-e in mouse brain crude lysate (50μg of protein loaded). Rabbit G13-X Aff - Purified Hu, Ms, Rt C, E, IP, P, WB
50 µl / €300.00
  DB Biotech, spol. s r.o.

Sample sizes available

PRKCE antibody

Western blot analysis of extracts of various cell lines, using PRKCE antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRKCE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKCE (RC217702, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKCE. Mouse IgG1 OTI1G5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRKCE antibody

Anti-PRKCE mouse monoclonal antibody (TA502367) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE(RC217702). Mouse IgG1 OTI4D12 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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