PRKCH antibody

Principal name

PRKCH antibody

Alternative names for PRKCH antibody

PKC-eta, PKC eta, PKCL, PRKCL, PKC-L, Protein kinase C eta type

SwissProt ID

P23298 (Mouse), P24723 (Human), Q64617 (Rat)

Gene ID

5583 (PRKCH), 81749 (Prkch), 18755

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PRKCH antibody

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

General / background references:Brandlin I et al. Protein kinase C (PKC)eta-mediated PKC mu activation modulates ERK and JNK signal pathways. J Biol Chem 277:6490-6 (2002). PubMed PMID: 11741879
Thabard W et al. Protein kinase C delta and eta isoenzymes control the shedding of the interleukin 6 receptor alpha in myeloma cells. Biochem J 358:193-200 (2001). PubMed PMID: 11485567
Kashiwagi M et al. PKCeta associates with cyclin E/cdk2/p21 complex, phosphorylates p21 and inhibits cdk2 kinase in keratinocytes. Oncogene 19:6334-41 (2000). PubMed PMID: 11175348
Waldron RT et al. The pleckstrin homology domain of protein kinase D interacts preferentially with the eta isoform of protein kinase C. J Biol Chem 274:9224-30 (1999). PubMed PMID: 10092595

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRKCH antibody

Western blot analysis of Jurkat cell lysates with anti-PRKCH antibody (1:200). Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRKCH antibody

WB Suggested Anti-Prkch Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKCH antibody

WB Suggested Anti-PRKCH Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole CellPRKCH is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRKCH (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRKCH antibody

PRKCH polyclonal antibody (A02), Lot # 051128JC01 Western Blot analysis of PRKCH expression in A-431 ( Cat # L015V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRKCH antibody

Western Blot analysis of PRKCH expression in transfected 293T cell line (H00005583-T02) by PRKCH MaxPab polyclonal antibody.

Lane 1: PRKCH transfected lysate(75.13 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PRKCH antibody

PRKCH Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PRKCH antibody

PRKCH Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PRKCH antibody

PRKCH MaxPab rabbit polyclonal antibody. Western Blot analysis of PRKCH expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PRKCH antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCH polyclonal antibody ( Cat # PAB4538 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma . Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PRKCH antibody

PRKCH Rabbit Rt C, E, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

PRKCH (N-term) antibody

Human Jurkat; WB Suggested Anti-PRKCH Antibody. . Titration: 1.0 ug/ml. . Positive Control: Jurkat Whole Cell. PRKCH is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; PRKCH antibody - N-terminal region (ARP56703_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

PRKCH (C-term) antibody

Mouse Brain; WB Suggested Anti-Prkch Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Brain; Prkch antibody - C-terminal region (ARP56704_P050) in Mouse Brain cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

PRKCH antibody

PRKCH Rabbit Aff - Purified Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

PRKCH antibody

Immunohistochemistry-Paraffin: PKC eta Antibody [NBP1-80899] - Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neurons of gyrus dentatus. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

PRKCH antibody

Peptide Competition and Phosphatase Treatment  Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-9) or treated with lambda (l) phosphatase (10), blocked with a 5% BSA-TBST buffer overnight at 4oC, and incubated with 0.35 mg/mL PKCh [pT655] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 10), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-9). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP-conjugate and bands were detected using the Pierce SuperSignal(TM) method.  The data show that the peptide corresponding to PKCh [pT655] blocks the antibody signal. The antibody signal was not blocked by the peptides corresponding to PKC isoforms a [pT638], bI [pT642], bI [pT641], g [pT655] and z [pT560], thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRKCH

PRKCH Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PRKCH

PRKCH Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

PRKCH

PRKCH Human
2 µg / €300.00
  Abnova Taiwan Corp.

PRKCH

PRKCH Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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