PRL3 / PTP4A3 antibody

Principal name

PRL3 / PTP4A3 antibody

Alternative names for PRL3 / PTP4A3 antibody

PRL-3, PRL-R, Protein tyrosine phosphatase type IVA 3, Protein-tyrosine phosphatase 4a3, Protein-tyrosine phosphatase of regenerating liver 3

SwissProt ID

A2VDT1 (Bovin), O75365 (Human), Q9D658 (Mouse)

Gene ID

11156 (PTP4A3), 100131062 (LOC100131062)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PRL3 / PTP4A3 antibody

PTP4A3 belongs to a small class of prenylated protein tyrosine phosphatases (PTPs). PTPs are cell sigling molecules that play regulatory roles in a variety of cellular processes. This class of PTPs contains a PTP domain and a characteristic C-termil prenylation motif. Studies of this class of PTPs in mice demonstrated that they were prenylated proteins in vivo, which suggested their association with cell plasma membrane. Overexpression of this gene in mammalian cells was reported to inhibit angiotensin-II induced cell calcium mobilization and promote cell growth.The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs). PTPs are cell sigling molecules that play regulatory roles in a variety of cellular processes. This class of PTPs contain a PTP domain and a characteristic C-termil prenylation motif. Studies of this class of PTPs in mice demonstrated that they were prenylated proteins in vivo, which suggested their association with cell plasma membrane. Overexpression of this gene in mammalian cells was reported to inhibit angiotensin-II induced cell calcium mobilization and promote cell growth. Two altertively spliced variants exist.

General readings

Kato, H. et al., Clin. Cancer Res. 10 (21): 7318-7328 (2004)
Wu, X. et al., Am. J. Pathol. 164 (6): 2039-2054 (2004)
Kim, K.A. et al., FEBS Lett. 565 (1-3): 181-187 (2004)
Kozlov, G. et al., J. Biol. Chem. 279 (12): 11882-11889 (2004)
Bardelli, A. et al., Clin. Cancer Res. 9 (15): 5607-5615 (2003)
Saha, S. et al., Science 294 (5545): 1343-1346 (2001)
Matter, W.F. et al., Biochem. Biophys. Res. Commun. 283 (5): 1061-1068 (2001)
Zeng, Q. et al., J. Biol. Chem. 275 (28): 21444-21452 (2000)
Zeng, Q. et al., Biochem. Biophys. Res. Commun. 244 (2): 421-427 (1998)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRL3 / PTP4A3 antibody

Predicted band size: 20 kDa . Positive control: LX-2 cells transfected with PRL-3 lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1: LX-2 cells transfected with PcDNA3.1 lysate Lane2,3,4: LX-2 cells transfected with 1.g; 2.g; 3.g PcDNA3.1-PRL-3 lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRL3 / PTP4A3 antibody

WB Suggested Anti-PTP4A3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate PTP4A3 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRL3 / PTP4A3 antibody

Figure 1. Western blot using anti-PRL3 (Cat. #AM11084PU-N) to detect PRL3 in 293 cells transfected with PRL3. Mouse IgG1 111AT714 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRL3 / PTP4A3 antibody

Figure 1. Western blot analysis of extract from LX-2 cells transfected with PRL-3, using PRL-3 antibody (#AP09464PU). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PRL3 / PTP4A3 antibody

Figure 1. Western blot analysis of extract from LX-2 cells transfected with PRL-3, using PRL-3 antibody (#AP09464PU). Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

PRL3 / PTP4A3 antibody

Western blot analysis of PRL3 (arrow) in mouse heart tissue lysates (35ug/lane) using PRL3 / PTP4A3 antibody Cat.-No. AP15252PU-N. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRL3 / PTP4A3 (C-term) antibody

Western blot analysis of PRL3/ PTP4A3 (arrow) in MCF-7 and 293 cell line lysates (35ug/lane) using PRL3 / PTP4A3 antibody Cat.-No. AP15253PU-N (C-term). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRL3 / PTP4A3 antibody

PRL3 / PTP4A3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRL3 / PTP4A3 antibody

PRL3 / PTP4A3 Rabbit Hu, Ms E
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRL3 / PTP4A3 antibody

PRL3 / PTP4A3 Rabbit Hu E
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRL3 / PTP4A3 antibody

PTP4A3 polyclonal antibody ( Cat # PAB6104 ) staining ( 2 µg/ml ) of human pancreas lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PRL3 / PTP4A3 (C-term) antibody

PRL3 / PTP4A3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PRL3 / PTP4A3 antibody

Human 293T; WB Suggested Anti-PTP4A3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. PTP4A3 is supported by BioGPS gene expression data to be expressed in HEK293T.; PTP4A3 antibody - middle region (ARP51193_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PRL3 / PTP4A3 antibody

Western Blot: PRL3 / PTP4A3 Antibody[NB100-877]-(0.03ug/ml) of Human Pancreas lysate (RIPA buffer, 30ug total protein per lane). Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRL3 / PTP4A3 (transcript variant 1)

PRL3 / PTP4A3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PRL3 / PTP4A3 (transcript variant 2)

PRL3 / PTP4A3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PRL3 / PTP4A3 (1-173, His-tag)

Recombinant human PRL-3, 1-173 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

PRL3 / PTP4A3 (1-173, His-tag)

Recombinant human PRL-3, 1-173 aa, His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

PRL3 / PTP4A3

PRL3 / PTP4A3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PRL3 / PTP4A3

PRL3 / PTP4A3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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