PRMT1 antibody

Principal name

PRMT1 antibody

Alternative names for PRMT1 antibody

HMT2, HRMT1L2, IR1B4, Protein arginine N-methyltransferase 1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4

SwissProt ID

Q63009 (Rat), Q6PFN7, Q99873 (Human), Q9JIF0 (Mouse), Q9NZ06 (Human), Q9URX7 (Schpo)

Gene ID

3276, 15469 (Prmt1), 321974 (prmt1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Hst (Hamster), Mky (Monkey), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PRMT1 antibody

Arginine methylation is an irreversible post translational modification that has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

General readings

General / background references:Cata J et al. Sam68 RNA binding protein is an in vivo substrate for protein arginine N-methyltransferase 1. Mol Biol Cell 14:274-87 (2003). PubMed PMID: 12529443
Frankel A et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J Biol Chem 277:3537-43 (2002). PubMed PMID: 11724789
Rho J et al. Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family. J Biol Chem 276:11393-401 (2001). PubMed PMID: 11152681
Frankel A & Clarke S PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain. J Biol Chem 275:32974-82 (2000). PubMed PMID: 10931850

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRMT1 antibody

WB Suggested Anti-PRMT1 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT1 antibody

Western blot detection of PRMT1 in Hela,A549 and SW480 cell lysates using PRMT1 mouse mAb (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa. Mouse IgG2b 4E9-H9-H12 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRMT1 (C-term) antibody

Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRMT1 (C-term) antibody

Figure 2. Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PRMT1 antibody (C-term) (Cat. #AP11003PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRMT1 (C-term) antibody

Human Lung, Respiratory Epithelium (formalin-fixed, paraffin-embedded) stained with PRMT1 antibody AP22791PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PRMT1 (C-term) antibody

Human Lung, Respiratory Epithelium (formalin-fixed, paraffin-embedded) stained with PRMT1 antibody AP22792PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Ig Fraction Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PRMT1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRMT1 antibody

PRMT1 Rabbit Hu, Ms WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

PRMT1 antibody

Human Kidney; Rabbit Anti-PRMT1 Antibody. Catalog Number: ARP40198. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PRMT1 antibody - middle region (ARP40198_T100) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

PRMT1 antibody

Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - See Notes, IHC Caption A Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PRMT1 antibody

PRMT1 Mouse IgG1 PRMT1-171 Purified Bov, Can, Chk, Hu, Mky, Ms, Rt E, IP, WB
50 µl / €340.00
  Novus Biologicals Inc.

PRMT1 antibody

Immunohistochemistry: PRMT1 Antibody (EPR3292) [NBP1-40673] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PRMT1 Rabbit IgG EPR3292 Hu, Ms P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

PRMT1 antibody

PRMT1 Rabbit Purified Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRMT1 (transcript variant 1)

PRMT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PRMT1 (Isoform 2) (1-353, His-MBP-tag)

Mouse PRMT1 isoform 2: 15% SDS-PAGE (3 µg) Mouse Purified ≥ 90 by SDS-PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

PRMT1 (Isoform 2) (1-353, His-MBP-tag)

Mouse PRMT1 isoform 2: 15% SDS-PAGE (3 µg) Mouse Purified ≥ 90 by SDS-PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

PRMT1 (Isoform 3) (1-353, His-MBP-tagged)

Human PRMT1 isoform 3: 15 %SDS-PAGE (3 µg) Human Purified ≥ 90 by SDS PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

PRMT1 (Isoform 3) (1-353, His-MBP-tagged)

Human PRMT1 isoform 3: 15 %SDS-PAGE (3 µg) Human Purified ≥ 90 by SDS PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

PRMT1

PRMT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PRMT1

PRMT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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