PRMT3 antibody

Principal name

PRMT3 antibody

Alternative names for PRMT3 antibody

HRMT1L3, Protein arginine N-methyltransferase 3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein

SwissProt ID

O13648 (Schpo), O60678 (Human), O70467 (Rat), Q0WVD6 (Arath), Q922H1 (Mouse)

Gene ID

10196 (PRMT3)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Paraffin Sections (P)

Background of PRMT3 antibody

Posttranslational modifications of proteins play an important role in the regulation of protein function, stability and localization. Such modifications occur on different amino acids and include phosphorylation, glycosylation, acetylation, or methylation. Arginine methylation is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. In type I PRMTs, the methylate arginine is found in different motifs such as the Arg-Gly-Gly-rich region (RGG motif) in many RNA binding proteins or the Arg-Xaa-Arg motif in poly(A)- binding protein II. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT3 is expressed in several tissues such as heart, small intestine, kidney, ovary, testis, thyroid, and cortex with a cytoplasmic localization within the cells. PRMT3 consists of two important domains in its N-terminal region (a C2H2 zinc-finger motif and a tyrosine-phosphorylation consensus sequence), which are probably important for regulation and catalytic activity.

General readings

General / background references:Frankel A et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J Biol Chem 277:3537-43 (2002). PubMed PMID: 11724789
Rho J et al. Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family. J Biol Chem 276:11393-401 (2001). PubMed PMID: 11152681
Frankel A & Clarke S PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain. J Biol Chem 275:32974-82 (2000). PubMed PMID: 10931850
Tang J et al. PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation. J Biol Chem 273:16935-45 (1998). PubMed PMID: 9642256

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRMT3 (468-421) antibody

(1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT3 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and cervical cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PRMT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRMT3 antibody

WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRMT3 antibody

WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRMT3 (N-term) antibody

Figure 2. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 antibody (N-term) (Cat. #AP11007PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRMT3 (C-term) antibody

Figure 3. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) (Cat.#AP11008PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRMT3 antibody

HRMT1L3 polyclonal antibody (A01), Lot # 061025JCS1 Western Blot analysis of HRMT1L3 expression in 293 ( Cat # L026V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRMT3 antibody

PRMT3 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

PRMT3 antibody

PRMT3 monoclonal antibody (M16), clone 3G4. Western Blot analysis of PRMT3 expression in HepG2(Cat # L019V1 ). Mouse IgG1 3G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PRMT3 antibody

Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PRMT3 polyclonal antibody ( Cat # PAB2171 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRMT3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRMT3 antibody

Human HepG2; WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; PRMT3 antibody - middle region (ARP32141_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PRMT3 antibody

Human Muscle; WB Suggested Anti-PRMT3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; PRMT3 antibody - middle region (ARP40183_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PRMT3 antibody

PRMT3 Mouse IgG3 PRMT3-367 Purified Hu, Ms, Rt E, ICC/IF, WB
50 µl / €360.00
  Novus Biologicals Inc.

PRMT3 antibody

Western Blot: PRMT3 Antibody [NBP1-55399] - This Anti-PRMT3 antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

PRMT3 antibody

Immunohistochemistry-Paraffin: PRMT3 Antibody [NBP1-83205] - Immunohistochemical staining of human cerebral cortex shows strong nuclear weak to moderate cytoplasmic positivity in neuronal cells and glial cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRMT3 (transcript variant 1)

PRMT3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PRMT3 (1-531, His-tag)

PRMT3 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

PRMT3 (1-531, His-tag)

PRMT3 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

PRMT3

PRMT3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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