PRMT5 antibody

Principal name

PRMT5 antibody

Alternative names for PRMT5 antibody

HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, Jak-binding protein 1, Protein Arginine N-Methyltransferase 5, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5

SwissProt ID

A7YW45 (Bovin), O14744 (Human), Q8CIG8 (Mouse)

Gene ID

10419 (PRMT5), 27374 (Prmt5)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of PRMT5 antibody

Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

General readings

Zhao Q, Rank G, Tan YT, Li H, Moritz RL, Simpson RJ, Cerruti L, Curtis DJ, Patel DJ, Allis CD, Cunningham JM, Jane SM.
PRMT5-mediated methylation of histone H4R3 recruits DNMT3A, coupling histone and DNA methylation in gene silencing.
Nature structural & molecular biology 2009 Mar 16 (3): 304-11.
PMID: 19234465

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRMT5 antibody

  PRMT5 mouse monoclonal antibody, clone OTI4G7 (formerly 4G7)  
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807462) Mouse IgG1 OTI4G7 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRMT5 antibody

  PRMT5 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)  
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807463) Mouse IgG2b OTI2C12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRMT5 antibody

  PRMT5 mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807621)(1:150) Mouse IgG2a OTI2H3 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRMT5 antibody

  Goat Anti-PRMT5 Antibody  
TA305754 staining (2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

  Rabbit polyclonal anti-PRMT-5 antibody  
Western blot analysis of PRMT-5 with lysate from rat kidney (Lane 1), 3T3 cells (Lane 2) and Jurkat cells (Lane 3 & 4). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

  Anti-PRMT5 Rabbit Polyclonal Antibody  
Predicted band size: 71 kDa . Positive control: K562, Hela and 293T cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

  PRMT5 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PRMT5 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

  Rabbit Polyclonal Anti-PRMT5 Antibody  
WB Suggested Anti-PRMT5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate.PRMT5 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

AP08809PU-N staining of PRMT5 in Testis tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded). Rabbit IgG Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
50 µl / €530.00
  Acris Antibodies GmbH

PRMT5 (C-term) antibody

Western blot analysis of anti-PRMT5 Antibody (C-term) (Cat.#AP11010PU-N) in HL60 cell line lysates (35ug/lane).PRMT5(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRMT5 (C-term) antibody

Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PRMT5 - human gene knockout kit via CRISPR

PRMT5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PRMT5 - human gene knockout kit via CRISPR

PRMT5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRMT5 - human gene knockout kit via CRISPR

PRMT5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRMT5 - human gene knockout kit via CRISPR

PRMT5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prmt5 - mouse gene knockout kit via CRISPR

Prmt5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Prmt5 - mouse gene knockout kit via CRISPR

Prmt5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prmt5 - mouse gene knockout kit via CRISPR

Prmt5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prmt5 - mouse gene knockout kit via CRISPR

Prmt5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRMT5 (transcript variant 1)

  Recombinant protein of human protein arginine methyltransferase 5 (PRMT5), transcript variant 1  
PRMT5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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