PRMT5 antibody

Principal name

PRMT5 antibody

Alternative names for PRMT5 antibody

HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, Jak-binding protein 1, Protein Arginine N-Methyltransferase 5, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5

SwissProt ID

A7YW45 (Bovin), O14744 (Human), Q8CIG8 (Mouse)

Gene ID

10419 (PRMT5)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Hst (Hamster), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of PRMT5 antibody

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.

General readings

General / background references:Frankel A et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J Biol Chem 277:3537-43 (2002). PubMed PMID: 11724789
Rho J et al. Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family. J Biol Chem 276:11393-401 (2001). PubMed PMID: 11152681
Frankel A & Clarke S PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain. J Biol Chem 275:32974-82 (2000). PubMed PMID: 10931850

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRMT5 antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807462) Mouse IgG1 OTI4G7 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRMT5 antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807463) Mouse IgG2b OTI2C12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRMT5 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PRMT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807621)(1:150) Mouse IgG2a OTI2H3 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PRMT5 antibody

TA305754 staining (2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

Western blot analysis of PRMT-5 with lysate from rat kidney (Lane 1), 3T3 cells (Lane 2) and Jurkat cells (Lane 3 & 4). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

Predicted band size: 71 kDa . Positive control: K562, Hela and 293T cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

Predicted band size: 71 kDa . Positive control: K562, Hela cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

Western blot analysis of extracts of various cell lines, using PRMT5 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

WB Suggested Anti-PRMT5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate.PRMT5 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT5 antibody

AP08809PU-N staining of PRMT5 in Testis tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded). Rabbit IgG Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PRMT5 (C-term) antibody

Western blot analysis of anti-PRMT5 Antibody (C-term) (Cat.#AP11010PU-N) in HL60 cell line lysates (35ug/lane).PRMT5(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRMT5 (C-term) antibody

Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PRMT5 antibody

Western Blot analysis of PRMT5 expression in transfected 293T cell line (H00010419-T01) by PRMT5 MaxPab polyclonal antibody.

Lane 1: SKB1 transfected lysate(70.07 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PRMT5 antibody

PRMT5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PRMT5 antibody

Western Blot analysis of PRMT5 expression in transfected 293T cell line (H00010419-T02) by PRMT5 MaxPab polyclonal antibody.

Lane 1: PRMT5 transfected lysate(72.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PRMT5 antibody

PRMT5 Mouse IgG2b 4F2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PRMT5 antibody

Western blot analysis of PRMT5 in cell lysates from HeLa cells. Using PRMT5 polyclonal antibody ( Cat # PAB0221 ) at 1 : 500. 20 ug of HeLa cell lysate was loaded per well of a mini gel. Rabbit Hu WB
0.2 ml / €400.00
  Abnova Taiwan Corp.

PRMT5 antibody

The PRMT5 polyclonal antibody ( Cat # PAB2175 ) is used in Western blot to detect PRMT5 in HL-60 cell lysate. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PRMT5 antibody

Human Kidney; Rabbit Anti-PRMT5 Antibody. Catalog Number: ARP40185. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PRMT5 antibody - N-terminal region (ARP40185_T100) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

PRMT5 antibody

Western Blot: PRMT5 Antibody [NB110-40570] - Detection of Human PRMT5 by Western Blot of  Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells.  Antibodies:  Affinity purified  rabbit anti-PRMT5 antibody NB110-40570  used for IP at 3 mcg/mg lysate. PRMT5 was also  immunoprecipitated by other rabbit anti-PRMT5 antibodies and NB110-40571, which recognize  downstream epitopes.  Detection: Chemiluminescence with an exposure time of 10 sec. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

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