PRMT6 / HRMT1L6 antibody

Principal name

PRMT6 / HRMT1L6 antibody

Alternative names for PRMT6 / HRMT1L6 antibody

Protein arginine N-methyltransferase 6, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6

SwissProt ID

Q5E9L5 (Bovin), Q6NZB1 (Mouse), Q96LA8 (Human)

Gene ID

55170 (PRMT6), 99890 (Prmt6)

Available reactivities

Hu (Human), Eq (Equine), Mky (Monkey), Ms (Mouse), Prim (Primate), Bov (Bovine), Can (Canine), Rt (Rat), Por (Porcine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of PRMT6 / HRMT1L6 antibody

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT6 was identified by searching the human genome for protein arginine N-methyltransferase (PRMT) family members. PRMT6 cDNA codes for a protein of approximate molecular weight of 42 kDa consisting of a catalytic core sequence common to other PRMT enzymes. PRMT6 demonstrates type I PRMT activity, capable of forming both >NG-monomethylarginine and asymmetric >NG,NG-dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. A comparison of substrate specificity reveals that PRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition to methylating substrate proteins, PRMT6 displays automethylation activit. GFP-fusion protein expression in the transfected cell lines suggest that PRMT6 localizes in the nucleus.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRMT6 / HRMT1L6 antibody

  Goat Anti-PRMT6 Antibody  
(0.5ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT6 / HRMT1L6 antibody

  Rabbit polyclonal anti-PRMT6 antibody  
Western blot analysis of PRMT6 using Jurkat cell lysate (Lane1 & 2). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRMT6 / HRMT1L6 antibody

  Rabbit Polyclonal PRMT6 Antibody  
Western Blot: PRMT6 Antibody [TA336434] - Analysis of PRMT6 in HeLa lysate using PRMT6 antibody at 1:500 dilution. Rabbit Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

PRMT6 / HRMT1L6 antibody

  Rabbit Polyclonal Anti-PRMT6 Antibody  
WB Suggested Anti-PRMT6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PRMT6 / HRMT1L6 antibody

  Mouse Monoclonal PRMT6 Antibody  
Western blot detection of PRMT6 in Hel and 293T cell lysates using PRMT6 mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size: 42KDa. Mouse IgG1 2C3-F1-G1 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PRMT6 / HRMT1L6 antibody

Western blot analysis using PRMT6 mouse mAb against A431 (1), Hela (2), A549 (3) and HEK293 (4) cell lysate. Mouse IgG1 4G2 Ascites Hu, Mky E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

PRMT6 / HRMT1L6 (23-43) antibody

Formalin-Fixed, Paraffin-Embedded Human Breast tissue stained with PRMT6 Antibody Cat.-No AP07178PU-N at 1/100 dilution after heat-induced antigen retrieval. Rabbit IgG Purified Elephant, Hu, Mky, Ms P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PRMT6 / HRMT1L6 (N-term) antibody

Figure 2. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PRMT6 - human gene knockout kit via CRISPR

PRMT6 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PRMT6 - human gene knockout kit via CRISPR

PRMT6 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRMT6 - human gene knockout kit via CRISPR

PRMT6 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRMT6 - human gene knockout kit via CRISPR

PRMT6 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prmt6 - mouse gene knockout kit via CRISPR

Prmt6 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Prmt6 - mouse gene knockout kit via CRISPR

Prmt6 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prmt6 - mouse gene knockout kit via CRISPR

Prmt6 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prmt6 - mouse gene knockout kit via CRISPR

Prmt6 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PRMT6 / HRMT1L6

  Recombinant protein of human protein arginine methyltransferase 6 (PRMT6)  
PRMT6 / HRMT1L6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PRMT6 / HRMT1L6

  Recombinant protein of human protein arginine methyltransferase 6 (PRMT6).  
PRMT6 / HRMT1L6 Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PRMT6 overexpression lysate

PRMT6 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

PRMT6 overexpression lysate

PRMT6 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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