Profilin-1 / PFN1 antibody

Principal name

Profilin-1 / PFN1 antibody

Alternative names for Profilin-1 / PFN1 antibody

Profilin I

SwissProt ID

P02584 (Bovin), P07737 (Human), P62962 (Mouse), P62963 (Rat)

Gene ID

5216 (PFN1)

Available reactivities

Hu (Human), Marsupials, Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)

Background of Profilin-1 / PFN1 antibody

The dymic polymerization and depolymerization of actin filaments, a process governed by exterl and interl sigling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dymics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP . Profilins bind to proteins involved in the regulation of actin dymics including palladin, dymin-1, VASP and N-WASP. In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development. Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth , membrane trafficking and endocytosis. The recently cloned profilin-3 is expressed in kidney and testes.

General readings

Kwiatkowski DJ, et al. (1988) J. Biol. Chem. 263(12): 5910-5.
Harbeck B., et al. (2000) J. Biol. Chem. 275(40): 30817-25.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Profilin-1 / PFN1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501211, Dilution 1:50) Mouse IgG2a OTI1D5 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Profilin-1 / PFN1 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50) Mouse IgG1 OTI2D2 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Profilin-1 / PFN1 antibody

Western Blot: Profilin Antibody - Western Blot on HeLa whole cell extracts. Rabbit Purified Bov, Hu, Ms, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

Predicted band size: 15 kDa . Positive control: Jurkat and 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

Predicted band size: 15 kDa . Positive control: Hela, HT-29 and A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: HT-29 cell lysate Lane3: A549 cell lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

Western blot analysis of extracts of various cell lines, using PFN1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

PFN1 polyclonal antibody (A01), Lot # Abnova060427QCS1. Western Blot analysis of PFN1 expression in Raw 264.7. Mouse Serum Hu, Ms E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Profilin-1 / PFN1 antibody

PFN1 polyclonal antibody (A02), Lot # 051129JC01 Western Blot analysis of PFN1 expression in Jurkat ( Cat # L017V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Profilin-1 / PFN1 antibody

Human Liver; WB Suggested Anti-PFN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; PFN1 antibody - N-terminal region (ARP48269_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Profilin-1 / PFN1

Profilin-1 / PFN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 (1-140)

Profilin-1 / PFN1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

Profilin-1 / PFN1 (1-140)

Profilin-1 / PFN1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

Profilin-1 / PFN1

Profilin-1 / PFN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Profilin-1 / PFN1

Profilin-1 / PFN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Profilin-1 / PFN1

Profilin-1 / PFN1 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

Profilin-1 / PFN1

Profilin-1 / PFN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Profilin-1 / PFN1

Profilin-1 / PFN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PFN1 293T Cell Transient Overexpression Lysate(Denatured)

PFN1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

PFN1 overexpression lysate

PFN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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