Profilin-1 / PFN1 antibody

Principal name

Profilin-1 / PFN1 antibody

Alternative names for Profilin-1 / PFN1 antibody

Profilin I

SwissProt ID

P02584 (Bovin), P07737 (Human), P62962 (Mouse), P62963 (Rat)

Gene ID

5216 (PFN1), 64303 (Pfn1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Por (Porcine), Rt (Rat), Can (Canine), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of Profilin-1 / PFN1 antibody

The dymic polymerization and depolymerization of actin filaments, a process governed by exterl and interl sigling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dymics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP . Profilins bind to proteins involved in the regulation of actin dymics including palladin, dymin-1, VASP and N-WASP. In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development. Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth , membrane trafficking and endocytosis. The recently cloned profilin-3 is expressed in kidney and testes.

General readings

Kwiatkowski DJ, et al. (1988) J. Biol. Chem. 263(12): 5910-5.
Harbeck B., et al. (2000) J. Biol. Chem. 275(40): 30817-25.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Profilin-1 / PFN1 antibody

  anti-PFN1 (Profilin 1) mouse monoclonal antibody, clone OTI1D5 (formerly 1D5)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501211, Dilution 1:50) Mouse IgG2a OTI1D5 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Profilin-1 / PFN1 antibody

  anti-PFN1 (Profilin 1) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)  
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50) Mouse IgG1 OTI2D2 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Profilin-1 / PFN1 antibody

  Rabbit Polyclonal Profilin 1 Antibody  
Western Blot: Profilin Antibody - Western Blot on HeLa whole cell extracts. Rabbit Purified Bov, Hu, Ms, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

  Anti-PFN1 Rabbit Polyclonal Antibody  
Predicted band size: 15 kDa . Positive control: Jurkat and 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

  Anti-PFN1 Rabbit Polyclonal Antibody  
Predicted band size: 15 kDa . Positive control: Hela, HT-29 and A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: HT-29 cell lysate Lane3: A549 cell lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

  PFN1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PFN1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

  Rabbit Polyclonal Anti-PFN1 Antibody  
WB Suggested Anti-PFN1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 antibody

  Rabbit Polyclonal Anti-PFN1 Antibody  
Profilin-1 / PFN1 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

PFN1 - human gene knockout kit via CRISPR

PFN1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PFN1 - human gene knockout kit via CRISPR

PFN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PFN1 - human gene knockout kit via CRISPR

PFN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PFN1 - human gene knockout kit via CRISPR

PFN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Profilin-1 / PFN1

  Recombinant protein of human profilin 1 (PFN1)  
Profilin-1 / PFN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Profilin-1 / PFN1 (1-140)

Profilin-1 / PFN1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

Profilin-1 / PFN1 (1-140)

Profilin-1 / PFN1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

PFN1 overexpression lysate

PFN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PFN1 (mGFP-tagged) - Human profilin 1 (PFN1), 200 uL, >10^7 TU/mL

Lenti ORF particles, PFN1 (mGFP-tagged) - Human profilin 1 (PFN1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, PFN1 (Myc-DDK tagged) - Human profilin 1 (PFN1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PFN1 (Myc-DDK tagged) - Human profilin 1 (PFN1) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pfn1 (GFP-tagged ORF) - Rat profilin 1 (Pfn1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pfn1 (GFP-tagged ORF) - Rat profilin 1 (Pfn1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Pfn1 (Myc-DDK-tagged ORF) - Rat profilin 1 (Pfn1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pfn1 (Myc-DDK-tagged ORF) - Rat profilin 1 (Pfn1), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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