Prohibitin-2 antibody

Principal name

Prohibitin-2 antibody

Alternative names for Prohibitin-2 antibody

PHB2, BAP, REA, BAP37, D-prohibitin, B-cell receptor-associated protein BAP37, Repressor of estrogen receptor activity

SwissProt ID

O35129 (Mouse), Q2HJ97 (Bovin), Q5XIH7 (Rat), Q5ZMN3 (Chick), Q99623 (Human)

Gene ID

11331 (PHB2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog, Dros (Drosophila)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C)

Background of Prohibitin-2 antibody

PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

General readings

Kategaya,L.S., et al. (2012) PLoS ONE 7 (2), E31987Emerson,V., et al. (2010) J. Virol. 84 (3), 1355-1365

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Prohibitin-2 (C-term) antibody

MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Prohibitin-2 (C-term) antibody

MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €190.00
  Acris Antibodies GmbH

Prohibitin-2 antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308685 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prohibitin-2 antibody

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463] - Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Prohibitin 2(NBP1-33463) antibody at 1:500 dilution. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Prohibitin-2 (N-term) antibody

Western blot analysis of PHB2 (arrow) using rabbit polyclonal PHB2 Antibody (Human C-term) (Cat.#TA300151). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Prohibitin-2 (88-299) antibody

Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Prohibitin-2 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (AP13987PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Prohibitin-2 antibody

Western blot analysis of PHB2 (arrow) using PHB2 Antibody (AP13988PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Prohibitin-2 pTyr128 antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Prohibitin-2 antibody

Prohibitin-2 Mouse IgG2a 2D3 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Prohibitin-2 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 polyclonal antibody ( Cat # PAB2840 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Prohibitin-2 antibody

Prohibitin-2 Rabbit Hu, Ms C, E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Prohibitin-2 antibody

Human MCF-7; WB Suggested Anti-PHB2 Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; PHB2 antibody - N-terminal region (ARP48315_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Prohibitin-2 antibody

Human HepG2; WB Suggested Anti-PHB2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. PHB2 is supported by BioGPS gene expression data to be expressed in HepG2.; PHB2 antibody - C-terminal region (ARP48316_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Prohibitin-2 antibody

Prohibitin-2 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

Prohibitin-2 antibody

Immunoprecipitation: REA Antibody [NB100-1807] - Detection of Human REA by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-REA antibodies NB100-1807, another REA Ab, NB100-1808, and NB100-1809 each used for IP at 3 mcg/mg lysate. Immunoprecipitated REA was blotted using BL1706 (upper blot) or BL1707 (lower blot).  Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

Prohibitin-2 antibody

Western Blot and Immunoprecipitation: REA Antibody [NB100-1809] - Detection of Human and Mouse REA on HeLa whole cell lysate using NB100-1809. REA was also immunoprecipitated using rabbit anti-REA antibodies NB100-1807 and NB100-1808. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Prohibitin-2 antibody

MCF-7 whole cell extract was  resolved by electrophoresis,  transferred to nitrocellulose and  probed with rabbit anti-BAP37  antibody. Proteins were visualized  using a donkey anti-rabbit secondary  conjugated to HRP and a  chemiluminescence detection  system. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Prohibitin-2 (N-term HIS tag, transcript variant 1)

Prohibitin-2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Prohibitin-2 (1-299, His-tag)

Prohibitin-2 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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