Prohibitin / PHB antibody

Principal name

Prohibitin / PHB antibody

Alternative names for Prohibitin / PHB antibody

PHB1

SwissProt ID

P35232 (Human), P67778 (Mouse), P67779 (Rat), P84173 (Chick), Q3T165 (Bovin), Q7T1D8

Gene ID

5245 (PHB), 18673 (Phb), 25344

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Prohibitin / PHB antibody

PHB(Prohibitin), also known as PHB1, is a protein that in humans is encoded by the PHB gene. White et al. (1991) mapped the PHB gene to chromosome 17 by analysis of human-mouse somatic cell hybrid cell lines using a genomic fragment of human prohibitin DNA isolated from a library using the rat prohibitin cDNA clone. By in situ hybridization, they localized the gene to 17q21. Sato et al. (1992) isolated the human homolog of the rat prohibitin gene and mapped it to 17q12-q21 by in situ hybridization. Proliferation of tumor cells depends on new blood vessel formation (angiogenesis) that accompanies malignant progression. Anticancer therapies using angiogenesis inhibitors or cytotoxic agents targeted to the vasculature of tumors have been evaluated in clinical trials. Although white fat is a nonmalignant tissue, it has the capability to quickly proliferate and expand. Furthermore, it is highly vascularized. Rupnick et al. (2002) showed that nonspecific angiogenesis inhibitors can prevent the development of obesity of mice.

General readings

Van Aken O. et al. (2007) Plant J. 52:850-864.Mishra S. et al. (2006) J Cell Mol Med. 10:353-363.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Prohibitin / PHB antibody

  PHB Rabbit Polyclonal Antibody  
Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 5mins), using C/EBP-a (phospho-Ser21) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining. Mouse IgG1 5H7 Ascites Hu, Mky, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Prohibitin / PHB antibody

Human Kidney, Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3F4-2B2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Prohibitin / PHB antibody

Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 5 ug/ml] Mouse IgG2a 3F4-2B2 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Prohibitin / PHB (C-term) antibody

  Rabbit Polyclonal Antibody against PHB (C-term)  
Western blot analysis of PHB1 (arrow) using rabbit polyclonal PHB1 Antibody (Human C-term) (Cat.#TA302260). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB (N-term) antibody

  Rabbit Polyclonal Antibody against PHB (N-term)  
PHB1 Antibody (Human N-term) (Cat. #TA302261) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

  Anti-PHB Rabbit Polyclonal Antibody  
Predicted band size: 30 kDa . Positive control: A431, HeLa and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A431 cell lysate Lane2: HeLa cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

  Rabbit Polyclonal anti-Prohibitin Antibody  
MCF7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-prohibitin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6031 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

  Mouse monoclonal anti-PHB antibody  
Western blot analysis of lysates from 293,MCF-7,HepG2,mouse NIH/3T3 cell line and rat liver tissue (from left to right),using USF1 Antibody (Center) Mouse IgG2b 1215CT487.109.106 Purified Hu, Ms, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

  Rabbit Polyclonal Anti-PHB Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-6: 293T cells, A549 cells, human hepatocellular carcinoma tissue, PC3 cells, hela cells, mouse heart tissue, Primary antibody: (PHB Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

  Rabbit Polyclonal anti-Prohibitin Antibody  
MCF7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-prohibitin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit Poly6031 Purified Hu WB
50 µl / €248.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

  Rabbit Polyclonal anti-Prohibitin Antibody  
Human Heart stained with anti-Prohibitin antibody. Rabbit Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB antibody

  Rabbit Polyclonal Prohibitin Antibody  
ELISA: Prohibitin Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Prohibitin / PHB (C-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Prohibitin / PHB (Center) antibody

Western blot analysis of anti-PHB Antibody (Center) (Cat.#AP12321PU-N) in Hela cell line lysates (35ug/lane). PHB (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Prohibitin / PHB antibody

Prohibitin / PHB Mouse IgG2a 4B12-2G12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Prohibitin / PHB antibody

Western blot analysis of PHB1 ( arrow ) using rabbit PHB polyclonal antibody ( Cat # PAB2547 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PHB1 gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Prohibitin / PHB antibody

Western blot analysis of PHB1 ( arrow ) using rabbit PHB polyclonal antibody ( Cat # PAB2548 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PHB1 gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Prohibitin / PHB antibody

Western blot analysis of PHB expression in Jurkat cell lysate. Rabbit Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Prohibitin / PHB antibody

Immunohistochemical staining of PHB on formalin fixed, paraffin embedded human Heart with PHB polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

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