Prolactin / PRL antibody

Principal name

Prolactin / PRL antibody

SwissProt ID

P01236 (Human), P01237 (Rat), P01238 (Pig), P01239 (Bovin), P01240 (Sheep), P06879 (Mouse), P12420 (Horse), P14676 (Chick), P46403 (Felca), Q28632 (Rabit)

Gene ID

5617 (PRL), 24683 (Prl), 19109, 443317

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Sh (Sheep), Sq (Squirrel), Can (Canine), Fe (Feline), Mky (Monkey), Ye (Yeast)

Available hosts

Mouse, Rabbit, Human, Rat

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Western blot / Immunoblot (WB), ELISA (capture) (E(capture)), Radioimmunoassay (R), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Flow Cytometry (F), Immunoprecipitation (IP), Luminex (LMNX)

Background of Prolactin / PRL antibody

The peptide hormones of the anterior pituitary are essential for growth, reproduction and intermediary metabolism. Their synthesis and secretion is controlled by hormones of hypothalamic origin, diseases and several pharmacological agents. In vertebrates 10 anterior pituitary hormones have been identified and are classified in to three classes based on their structural features. The growth hormone (GH), prolactin (Prl) and placental lactogen (PL) are members of somototropic hormone family. The glycoprotein hormone family comprise of Thyroid stimulating hormone (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH). The corticotrophin (ACTH), two melanocyte stimulating hormones (MSH) and the two lipotropins (LPH) are the members of hormones derived from POMC gene. The human growth hormone (hGH) is coded by one gene of a five gene cluster located on chromosome 17, the other genes code for two other variants of lactogen or a growth hormone variant expressed in placenta. The hGH is a single polypeptide chain wit 191 aminoa acids (22 kDa). The hGH has 2 disulfide bonds and it is not glycosylated. There exist a splice variant of hGH where amino acids 32-46 is not translated and this splice variant accounts up to 5-10% of the total hGH. Placenta also secrete a 22 kDa hGH that can be distinguished from the pituitary form but its significance is not clear. The amount of GH secreted in 24 hours is high in children reaches maximal levels at the time of adolescence then tapers off during adult hood. The secretion of GH is in pulses and occurs at irregular intervals. The amplitude of GH concentration is maximum at night shortly after onset of REM sleep. Somatotrophs in anterior pituitary have an intracellular Ca+2 oscillations that are believed to to be responsible for pulsatile secretion of GH (1). The release of GH is under direct control of GH release hormone (GHRH) which stimulates GH. Both GH and GHRH bind to their cognate GPCR leading to an increase (GHRH) or decrease (somatotropin) in cAMP and calcium levels. Several pharmacological agents can increase GH release via acting on hypothalamus.
The physiological effects of GH is via its interaction with cell surface receptor which are widely distributed throughout body. The GH receptor is a 620 amino acid single trans-membrane protein with large extracellular N-terminal domain that interacts with GH and a about 250 amino acid cytoplasmic end of the protein. The large extracellular domain of GH receptor is cleaved to acts as GH binding protein of about 60kDa. The GH has direct effect on lipid and carbohydrate metabolism, its anabolic growth promoting effects are mediated by somatomedins or insulin like growth factors (IGF-1 and IGF-2) which share significant homology (2). GH administration increase the production of IGF in liver, triglyceride hydrolysis and hepatic glucose output. IGF-1 also exerts indirect growth promoting effects of GH by increase chondrogenesis, skeletal and soft tissue growth.

General readings

Ben-jonathan N., et al. (1996) Endocr Rev. 17(6):639-69.Cooke NE., et al. (1981) J. Biol. Chem. 256, 4007-4016.Goffin V., et al. (1999) Mol Cell Endocrinol. 151, 79-87.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Prolactin / PRL antibody

Anti-PRL mouse monoclonal antibody (TA500718) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL(RC205627). Mouse IgG2b OTI9D12 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prolactin / PRL antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500719) Mouse IgG2a OTI6B1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Prolactin / PRL antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500720) Mouse IgG1 OTI11B11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prolactin / PRL antibody

Human pituitary stained with anti-prolactin antibody Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

Prolactin / PRL antibody

Human pituitary stained with anti-prolactin antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

Prolactin / PRL antibody

Human pituitary stained with anti-prolactin antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

Prolactin / PRL antibody

Predicted cell location: Cytoplasm . Positive control: Human tonsil tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using PRL antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prolactin / PRL antibody

Predicted cell location: Cytoplasm . Positive control: Human gastric cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using PRL antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prolactin / PRL antibody

Western blot analysis of extracts of various cell lines , using PRL antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

Prolactin / PRL antibody

Immunohistochemistry staining of Paraffin Pituitary tissue by Prolactin antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Rabbit Purified Hu P
1 ml / €650.00
  OriGene Technologies, Inc.

Prolactin / PRL antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Mouse IgG2a OTI2B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prolactin / PRL antibody

Anti-PRL mouse monoclonal antibody (TA500817) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL(RC205627). Mouse IgG2a OTI2E4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prolactin / PRL antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Mouse IgG1 OTI2C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prolactin / PRL antibody

ELISA: Prolactin Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Prolactin / PRL antibody

PRL Elisa with 2B7 Capture (TA600145) and 6B1 Detection (TA700145) Antibodies. Substrate used: Recombinant Human PRL (TP305627) Mouse IgG1 OTI2B7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Prolactin / PRL antibody

PRL Elisa with 2B7 Capture (TA600145) and 6B1 Detection (TA700145) Antibodies. Substrate used: Recombinant Human PRL (TP305627) Mouse IgG1 OTI6B1 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Prolactin / PRL antibody

Prolactin / PRL Mouse IgG1 9F3 Purified Hu E
1 mg / €370.00
  Acris Antibodies GmbH

Prolactin / PRL antibody

Prolactin / PRL Mouse IgG2a 090-11710 Purified Hu E
1 mg / €330.00
  Acris Antibodies GmbH

Prolactin / PRL antibody

Prolactin / PRL Mouse IgG1 090-18111 Purified Hu E
1 mg / €330.00
  Acris Antibodies GmbH

Prolactin / PRL antibody

Prolactin / PRL Mouse IgG1 PRL-1 Aff - Purified Hu E, E(capture)
0.5 mg / €430.00
  Acris Antibodies GmbH

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