Prominin-2 / PROM2 antibody

Principal name

Prominin-2 / PROM2 antibody

Alternative names for Prominin-2 / PROM2 antibody

Prominin 2, PROM-2, PROML2

SwissProt ID

Q3UUY6 (Mouse), Q8CJ52 (Rat), Q8N271 (Human)

Gene ID

150696 (PROM2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), Bov (Bovine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of Prominin-2 / PROM2 antibody

A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. Several criteria were applied to identify and characterize these new and novel genes. First, a biological signal sequence trap in yeast cells was utilized to identify cDNA clones encoding putative secreted proteins, second strategy utilized various algorithms that recognize features such as the hydrophobic properties of signal sequences to identify putative proteins encoded by expressed sequence tags (ESTs) from human cDNA libraries and finally, ESTs for protein sequence similarity to a set of known receptors and their ligands with the BLAST algorithm. Based on these criteria isolation of full length cDNA clones for each of these genes resulted in identification of more than 1000 novel proteins. One of these new genes is Prominin2. Prominin 2 is a 112 kDa glycoporotein structurally related to Prominin 1 (CD133) although amino acid similarity is not more than 30% but their genomic organization is strikingly similar (1). Like Prominin1, the prominin 2 exhibit similar membrane topology with 5 trans-membrane domains and two large glycosylated extracellular domains. Similar to Prominin1 localization, the Prominin 2 is also associated with membrane protrusions of the epithelial cells from adult kidney, and all along the digestive track and other epithelial tissues. One striking difference between Prominin1 and Prominin 2 expression lies in its conspicuous absence in eye tissue (2).
Prominin 2, along with other genes (AP1M2, MAL2, PRSS8 and FLJ20171) expression is differentially expressed in chromophobe renal cell carcinoma compared to benign oncocytoma consistently and this marker can be used to differentiate RCC form benign oncocytoma (3). Prominin 2 is an androgen-responsive genes proapoptotic membrane protein that is expressed in human prostate and human prostate cancer cell lines with highest levels in less aggressive LNCaP cell and low expression in highly aggressive PC3 and DU145 cells suggesting a role of Prominin 2 in down-regulation of aggressiveness of prostate cancer cells (4).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Prominin-2 / PROM2 antibody

Anti-PROM2 mouse monoclonal antibody (TA500310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PROM2(RC211605). Mouse IgG2b OTI5E5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prominin-2 / PROM2 antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-PROM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500331, Dilution 1:50) Mouse IgG1 OTI6B7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prominin-2 / PROM2 antibody

Immunofluorescent staining of Cos7 cells using anti-PROM2 mouse monoclonal antibody (TA500349). Mouse IgG2b OTI3D12 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Prominin-2 / PROM2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PROM2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500351, Dilution 1:50) Mouse IgG1 OTI14D1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prominin-2 / PROM2 antibody

Anti-PROM2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

Prominin-2 / PROM2 (N-term) antibody

Anti-PROM2 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

Prominin-2 / PROM2 antibody

Anti-PROM2 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

Prominin-2 / PROM2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2. Mouse IgG1 OTI5G8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prominin-2 / PROM2 antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-PROM2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500350, Dilution 1:50) Mouse IgG1 OTI13A9 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prominin-2 / PROM2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2. Mouse IgG2a OTI15F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Prominin-2 / PROM2 control peptide

Prominin-2 / PROM2
0.1 mg / €250.00
  Acris Antibodies GmbH

Prominin-2 / PROM2 (315-338) antibody

Prominin-2 / PROM2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Prominin-2 / PROM2 antibody

Fluorescence microscopy analysis of expression of PROM2 (red signal), a K-Ras dependency signature gene, in K-Ras-dependent (H358 and YAPC) and KRas- independent (PA-TU-8988T and A549) cell lines. Green signal corresponds to Factin, as revealed by phalloidin staining. Scale bar = 15μM. Cancer Cell. 2009 June 2; 15(6): 489–500. doi: 10.1016/j.ccr.2009.03.022. Mouse IgG2b Purified Hu F, ICC/IF
0.1 mg / €460.00
  Neuromics Antibodies

Lysates

Catalog No.    

PROM2 overexpression lysate

PROM2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

PROM2 overexpression lysate

PROM2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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