Pros54 / RPN10 antibody

Principal name

Pros54 / RPN10 antibody

Alternative names for Pros54 / RPN10 antibody

PROS-54, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, 54 kDa subunit of mu particle, Multiubiquitin chain-binding protein

SwissProt ID

P55035 (Drome)

Gene ID

40388 (Pros54)

Ncbi ID

NP_524204, 7227

Available reactivities

Dros (Drosophila)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Pros54 / RPN10 antibody

Pros54 / RPN10 Rabbit Purified Dros WB
0.1 ml / €640.00
  Abnova Taiwan Corp.

Pros54 / RPN10 antibody

3 µg of total protein from  Arabidopis thaliana leaves  extracted with CelLytic P (Sigma)  were separated on 10  % SDS-PAGE and blotted overnight at 30V to a nitrocellulose membrane. Blots were blocked with 1% western blocking reagent (Roche) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody (DS5a) at a dilution of 1:2000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, and then washed twice for 10 min with TBS-T at RT with agitation. Subsequently, the blot was washed twice with 0,5% western blocking reagent for 10 min each. Blot was incubated in secondary antibody (Goat anti-rabbit IgG (H&L) HRP conjugated, AS09 602 from Agrisera) diluted to 1:50 000 in 0,5% western blocking reagent for 1h at RT with agitation. The blot was washed 4 times with TBS-T buffer. Detection of HRP was performed with ECL according to the manufacturer's instructions (GE healthcare). Exposure time was 2 seconds. Position of protein on gel corresponds to expected size - 40 kDa.Courtesy Dr. Jozefus Schippers, Max Planck Institute, Germany. Rabbit Serum Arabidopsis, Dros WB
50 µl / €300.00
  AgriSera AB

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