Protein A antibody

Principal name

Protein A antibody

Available reactivities

Bac (Bacteria), All Species, S. aureus (Staphylococcus aureus), Hu (Human), Ms (Mouse)

Available hosts

Chicken, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of Protein A antibody

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. 
Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. 
Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).

General readings

General / background references:Blakeslee D & Baines MG Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG. J Immunol Methods 13:305-20 (1976). PubMed PMID: 13125

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Protein A antibody

Protein A Rabbit IgG Purified ID, WB
1 ml / €450.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat Serum S. aureus E, IP, WB
2 ml / €450.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat IgG Agarose
20 mg / €3,300.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat IgG Biotin C, E, WB
10 mg / €2,500.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat IgG Texas Red C, E, F, ICC/IF
20 mg / €3,300.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Protein A

Protein A S. aureus Purified > 95 % : This product is chromatographically pure Protein A and shows predominantly a single band by SDS-PAGE. Greater than 95% of the A280 material binds to Human IgG.
5 mg / €210.00
  Acris Antibodies GmbH

Protein A (AP conjugated)

Protein A S. aureus AP This product was prepared from chromatographically pure Protein A.
1 mg / €290.00
  Acris Antibodies GmbH

Protein A (Biotin conjugated)

Protein A Biotin
0.5 mg / €160.00
  Acris Antibodies GmbH

Protein A (FITC conjugated)

Protein A S. aureus FITC This product was prepared from chromatographically pure Protein A.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A.
5 mg / €280.00
  Acris Antibodies GmbH

Protein A (Peroxidase conjugated)

Dot Blot of Peroxidase Conjugated Protein A. Lane 1: Human IgG. Lane 2: Golden Syrian Hamster IgG. Lane 3: Sheep IgG. Load: 3-fold serial dilution starting at 200 ng. Primary Antibody: None. Secondary Antibody: Peroxidase Conjugated Protein A at 1/1000 for60 min at RT. Block: 1% BSA-TTBS 30 min at RT. S. aureus HRP This product was prepared from chromatographically pure Protein A.
2 mg / €290.00
  Acris Antibodies GmbH

Protein A (Sepharose)

Protein A S. aureus Sepharose
2 ml / €310.00
  Acris Antibodies GmbH

Protein A (Sepharose)

Protein A S. aureus Sepharose
5 ml / €450.00
  Acris Antibodies GmbH

Protein A (Sepharose)

Protein A S. aureus Sepharose
25 ml / €1,120.00
  Acris Antibodies GmbH

13 Item(s)

per page
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