Protein A antibody

Principal name

Protein A antibody

Available reactivities

Bac (Bacteria), All Species, S. aureus (Staphylococcus aureus), Hu (Human), Ms (Mouse)

Available hosts

Chicken, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of Protein A antibody

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. 
Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. 
Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).

General readings

General / background references:Blakeslee D & Baines MG Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG. J Immunol Methods 13:305-20 (1976). PubMed PMID: 13125

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Protein A antibody

Protein A Rabbit IgG Purified ID, WB
1 ml / €450.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat Serum S. aureus E, IP, WB
2 ml / €440.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat IgG Agarose
20 mg / €3,300.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat IgG Biotin C, E, WB
10 mg / €2,500.00
  Acris Antibodies GmbH

Protein A antibody

Protein A Goat IgG Texas Red C, E, F, ICC/IF
20 mg / €3,300.00
  Acris Antibodies GmbH

Protein A antibody

Western blot analysis using 10 ng recombinant Protein A. Rabbit IgG Hu, Ms WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Protein A antibody

Protein A Goat IgG Hu C, E, ID, IP, WB
1 mg / €390.00
  Abnova Taiwan Corp.

Protein A antibody

Western Blot: Protein A Antibody [FITC] [NB120-7244] Goat anti Protein A antibody (was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of ~1ug and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti Protein A antibody (1:5K in blocking buffer, ON 4 C). Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat 1:10K 1.5 hr RT in blocking buffer and imaged on the BioRad VersaDoc imaging system. Goat IgG FITC Bac E, ICC/IF, WB
5 mg / €1,090.00
  Novus Biologicals Inc.

Protein A antibody

Western Blot: Protein A Antibody [Biotin] [NB120-7246] Goat anti Protein A antibody (was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of ~1ug and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti Protein A antibody (1:5K in blocking buffer, ON 4 C). Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat 1:10K 1.5 hr RT in blocking buffer and imaged on the BioRad VersaDoc imaging system. Goat IgG Biotin Bac E, P, WB
5 mg / €1,260.00
  Novus Biologicals Inc.

Protein A antibody

Western Blot: Protein A Antibody [HRP] [NB600-1223] Goat anti Protein A antibody (was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of ~1ug and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti Protein A antibody (1:5K in blocking buffer, ON 4 C). Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat 1:10K 1.5 hr RT in blocking buffer and imaged on the BioRad VersaDoc imaging system. Goat IgG HRP All Species E, P, WB
2 mg / €510.00
  Novus Biologicals Inc.

Protein A antibody

Protein A Rabbit IgG Aff - Purified WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Protein A

Protein A S. aureus Purified > 95 % : This product is chromatographically pure Protein A and shows predominantly a single band by SDS-PAGE. Greater than 95% of the A280 material binds to Human IgG.
5 mg / €210.00
  Acris Antibodies GmbH

Protein A (AP conjugated)

Protein A S. aureus AP This product was prepared from chromatographically pure Protein A.
1 mg / €280.00
  Acris Antibodies GmbH

Protein A (Biotin conjugated)

Protein A Biotin
0.5 mg / €150.00
  Acris Antibodies GmbH

Protein A (FITC conjugated)

Protein A S. aureus FITC This product was prepared from chromatographically pure Protein A.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A.
5 mg / €270.00
  Acris Antibodies GmbH

Protein A (Peroxidase conjugated)

Dot Blot of Peroxidase Conjugated Protein A. Lane 1: Human IgG. Lane 2: Golden Syrian Hamster IgG. Lane 3: Sheep IgG. Load: 3-fold serial dilution starting at 200 ng. Primary Antibody: None. Secondary Antibody: Peroxidase Conjugated Protein A at 1/1000 for60 min at RT. Block: 1% BSA-TTBS 30 min at RT. S. aureus HRP This product was prepared from chromatographically pure Protein A.
2 mg / €280.00
  Acris Antibodies GmbH

Protein A (Sepharose)

Protein A S. aureus Sepharose
2 ml / €310.00
  Acris Antibodies GmbH

Protein A (Sepharose)

Protein A S. aureus Sepharose
5 ml / €430.00
  Acris Antibodies GmbH

Protein A (Sepharose)

Protein A S. aureus Sepharose
25 ml / €1,110.00
  Acris Antibodies GmbH

Kits

Catalog No. React. Range Assaytime  

Protein A Agarose Immunoprecipitation Kit

Protein A
20 reaction / €540.00
  Abnova Taiwan Corp.

20 Item(s)

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