Protein ajuba / JUB antibody

Principal name

Protein ajuba / JUB antibody

SwissProt ID

Q5U2Z2 (Rat), Q91XC0 (Mouse), Q96IF1 (Human)

Gene ID

84962 (AJUBA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Can (Canine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunocytochemistry (ICC)

Background of Protein ajuba / JUB antibody

Human Ajuba (also called JUB protein and ajuba homolog isoform 1) is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

General readings

Hirota T et al. Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells. Cell 114:585-98 (2003). PubMed PMID: 13678582

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Protein ajuba / JUB (224-239) antibody

WB using Anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head).  Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 µg of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody.  Detection occurred using a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L]  for 45 min at RT. Rabbit IgG Aff - Purified Can, Chimp, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Protein ajuba / JUB antibody

Western Blot analysis of JUB expression in transfected 293T cell line (H00084962-T01) by JUB MaxPab polyclonal antibody.

Lane 1: JUB transfected lysate(59.18 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Protein ajuba / JUB antibody

Western Blot analysis of JUB expression in transfected 293T cell line (H00084962-T01) by JUB MaxPab polyclonal antibody.

Lane 1: JUB transfected lysate(59.18 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Protein ajuba / JUB antibody

Immunofluorescence analysis of HeLa cells, using JUB polyclonal antibody ( Cat # PAB8477 ) . Rabbit Purified Hu ICC/IF, IP
25 µg / €660.00
  Abnova Taiwan Corp.

Protein ajuba / JUB antibody

Western blot using JUB polyclonal antibody ( Cat # PAB9930 ) shows detection of JUB-RFP fusion protein in cell lysates ( arrow-head ).
Lanes correspond to 1 ) vector only transfection, 2 ) human JUB-RFP, 3 ) mouse JUB-RFP, and 4 ) mock transfection.
Approximately 50 µg of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1 : 1,700 dilution of JUB polyclonal antibody.
Detection occurred using a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nmchannel, green ).
Molecular weight estimation wasmade by comparison to prestained MW markers ( indicated at left, 700 nm channel, red ).
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc. Chimp, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

Protein ajuba / JUB antibody

Protein ajuba / JUB Rabbit Aff - Purified Hu ICC, IP
25 µg / €630.00
  Funakoshi Co., Ltd.

Protein ajuba / JUB antibody

Ajuba Antibody [21G4] Mouse IgG1 21G4 Purified Hu IP, WB
0.1 ml / €400.00
  Immuquest

Protein ajuba / JUB antibody

Western Blot: Ajuba Antibody [NB600-468] - Affinity Purified Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates. Lanes correspond to 1) vector only transfection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Rabbit Aff - Purified Hu E, IP, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Protein ajuba / JUB (transcript variant 1)

Protein ajuba / JUB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Protein ajuba / JUB

Protein ajuba / JUB Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Protein ajuba / JUB

Protein ajuba / JUB Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

AJUBA overexpression lysate

AJUBA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

AJUBA overexpression lysate

AJUBA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

JUB 293T Cell Transient Overexpression Lysate(Denatured)

JUB 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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