Protein phosphatase 1A / PPM1A antibody
Principal name
Protein phosphatase 1A / PPM1A antibody
Alternative names for Protein phosphatase 1A / PPM1A antibody
PPPM1A, Protein phosphatase 2C isoform alpha, PP2C-alpha, Protein phosphatase IA
Ncbi ID
NP_066283.1, NP_066283, 9606, 5874, NP_808820, NM_177951, NP_808821, NP_032936.1, NM_017038, NM_021003, NM_177952
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Protein phosphatase 1A / PPM1A antibody
- Kit Component:
- - KN313708G1, Ppm1a gRNA vector 1 in pCas-Guide vector
- KN313708G2, Ppm1a gRNA vector 2 in pCas-Guide vector
- KN313708D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Das, A. K. et al. (1996) EMBO J. 15, 6798-6809.
Immunoblot analysis Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2C-alpha (1/250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PP2C-alpha(~43kDa)
Immunoperoxidase of monoclonal antibody to PPM1A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and PPM1A HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-PPM1A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunoblot analysis of mouse liver cytosol extract was resolved by electrophoresis , transferred to PVDF membrane and probed with PPM1A monoclonal antibody , clone p6c7 ( 1 : 250 ) ( Cat # MAB1050 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PPM1A ( ~ 43 kDa ) .
PPM1A monoclonal antibody, clone p6c7. Western blot analysis of PPM1 expression in human liver.
PPM1A monoclonal antibody, clone p6c7. Western blot analysis of PPM1 expression in Jurkat.
Western blotting of PP2C polyclonal antibody ( Cat # PAB10354 ) in total rat brain extract. Blocked with specific peptide (+). Note: PP2C polyclonal antibodyalso readily detects recombinant PP2C alpha and PP2C beta.
Human Heart; WB Suggested Anti-PPM1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; PPM1A antibody - middle region (ARP57765_P050) in Human Heart cells using Western Blot
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged PPM1A is 3 ng/ml as a capture antibody.
WB Suggested Anti-PPM1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA344616 | Protein phosphatase 1A / PPM1A antibody |
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| Rabbit Polyclonal Anti-PPM1A Antibody - middle region | |||||||
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Rabbit | IgG | Purified | Bov, Can, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€360.00
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| OriGene Technologies, Inc. | |||||||
