Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Rb (Rabbit), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Por (Porcine)
Available hosts
Mouse, Rabbit, Sheep
Available applications
Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Protein phosphatase 1A / PPM1A antibody
Kit Component:
- KN313708G1, Ppm1a gRNA vector 1 in pCas-Guide vector - KN313708G2, Ppm1a gRNA vector 2 in pCas-Guide vector - KN313708D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette - GE100003, scramble sequence in pCas-Guide vector
Immunoblot analysis Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2C-alpha (1/250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PP2C-alpha(~43kDa)
Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and PPM1A HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-PPM1A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunoblot analysis of mouse liver cytosol extract was resolved by electrophoresis , transferred to PVDF membrane and probed with PPM1A monoclonal antibody , clone p6c7 ( 1 : 250 ) ( Cat # MAB1050 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PPM1A ( ~ 43 kDa ) .
Western blotting of PP2C polyclonal antibody ( Cat # PAB10354 ) in total rat brain extract. Blocked with specific peptide (+). Note: PP2C polyclonal antibodyalso readily detects recombinant PP2C alpha and PP2C beta.
Human Heart; WB Suggested Anti-PPM1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; PPM1A antibody - middle region (ARP57765_P050) in Human Heart cells using Western Blot
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OriGene Technologies, Inc. (North America)
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Acris Antibodies GmbH has now officially been renamed to OriGene Technologies GmbH.
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