Protein phosphatase 1B / PPM1B antibody

Principal name

Protein phosphatase 1B / PPM1B antibody

Alternative names for Protein phosphatase 1B / PPM1B antibody

PP2CB, Protein phosphatase 2C isoform beta, PP2C-beta

SwissProt ID

O62830 (Bovin), O75688 (Human), P35815 (Rat), P36993 (Mouse)

Gene ID

5495 (PPM1B), 100043456 (Gm10681)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of Protein phosphatase 1B / PPM1B antibody

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Parvari R, et al., (2005) Genomics 86:195-211.
Marley AE, et al., (1998) FEBS Lett 431(1):121-124.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Protein phosphatase 1B / PPM1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Mouse IgG1 OTI3E10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1B monoclonal antibody. Mouse IgG1 OTI3F2 Purified Can, Hu, Mky, Rt F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

Anti-PPM1B mouse monoclonal antibody (TA502866) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1B(RC212918 ). Mouse IgG2a OTI3C9 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ARRB2 and PPM1B HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 and anti-PPM1B mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 1A3-2A4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Protein phosphatase 1B / PPM1B antibody

WB Suggested Anti-PPM1B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Protein phosphatase 1B / PPM1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Mouse IgG2b OTI2H5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Mouse IgG1 OTI3c2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Mouse IgG2a OTI3A10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PPM1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502900) Mouse IgG2a OTI3E8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Mouse IgG1 OTI2D9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Mouse IgG2a OTI3G9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Protein phosphatase 1B / PPM1B (1-192) antibody

Flow cytometry analysis of PPM1B in A549 cell line, staining at 2 - 5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Mouse IgG2b k1B1 Purified Hu E, F, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Protein phosphatase 1B / PPM1B (1-192) antibody

Flow cytometry analysis of PPM1B in A549 cell line, staining at 2 - 5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate. Mouse IgG2b k1B1 Purified Hu E, F, WB
50 µl / €230.00
  Acris Antibodies GmbH

Protein phosphatase 1B / PPM1B antibody

Western Blot analysis of PPM1B expression in transfected 293T cell line (H00005495-T01) by PPM1B MaxPab polyclonal antibody.

Lane 1: PPM1B transfected lysate(52.69 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Protein phosphatase 1B / PPM1B antibody

Western Blot analysis of PPM1B expression in transfected 293T cell line (H00005495-T01) by PPM1B MaxPab polyclonal antibody.

Lane 1: PPM1B transfected lysate(52.69 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Protein phosphatase 1B / PPM1B antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PPM1B and CHUK. HeLa cells were stained with anti-PPM1B rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Protein phosphatase 1B / PPM1B antibody

Cell lysates of MCF-7 ( 30 µg ) were resolved by SDS-PAGE and probed with PPM1B monoclonal antibody, clone k1B1 ( Cat # MAB2036 ) ( 1:2,000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b k1B1 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Protein phosphatase 1B / PPM1B antibody

Human Jurkat; WB Suggested Anti-PPM1B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; PPM1B antibody - N-terminal region (ARP57767_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Protein phosphatase 1B / PPM1B antibody

Western Blot and Immunoprecipitation: PPM1B Antibody [NB110-38886] - Detection of Human and Mouse PPM1B on HeLa whole cell lysate using NB110-38886. PPM1B was also immunoprecipitated by rabbit anti-PPM1B antibody NB110-38885. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Protein phosphatase 1B / PPM1B antibody

Protein phosphatase 1B / PPM1B Mouse IgG2b k1B1 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

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