Protein phosphatase 1F / PPM1F antibody

Principal name

Protein phosphatase 1F / PPM1F antibody

Alternative names for Protein phosphatase 1F / PPM1F antibody

KIAA0015, POPX2, Partner of PIX 2, Protein fem-2 homolog, CaM-kinase phosphatase, CaMKPase, Ca(2+)/calmodulin-dependent protein kinase phosphatase

SwissProt ID

P49593 (Human), Q8CGA0 (Mouse), Q9WVR7 (Rat)

Gene ID

9647 (PPM1F)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of Protein phosphatase 1F / PPM1F antibody

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008].

General readings

Tan KM, Chan SL, et al. (2001). J Biol Chem. 276(47):44193-202. Harvey BP, Banga SS, et al. (2004). J Biol Chem. 279(23):24889-98.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Protein phosphatase 1F / PPM1F (N-term) antibody

Western blot analysis of anti-PPM1F Pab (Cat. #TA302150) in mouse  liver tissue lysate (35ug/lane). PPM1F(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Protein phosphatase 1F / PPM1F antibody

The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT6D11 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Protein phosphatase 1F / PPM1F antibody

The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT6D11 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Protein phosphatase 1F / PPM1F (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Protein phosphatase 1F / PPM1F antibody

PPM1F polyclonal antibody (A01), Lot # 051024JC01 Western Blot analysis of PPM1F expression in K-562 ( Cat # L009V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Immunofluorescence of purified MaxPab antibody to PPM1F on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Immunofluorescence of purified MaxPab antibody to PPM1F on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Western Blot analysis of PPM1F expression in transfected 293T cell line (H00009647-T02) by PPM1F MaxPab polyclonal antibody.

Lane 1: PPM1F transfected lysate(49.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Western Blot analysis of PPM1F expression in transfected 293T cell line (H00009647-T02) by PPM1F MaxPab polyclonal antibody.

Lane 1: PPM1F transfected lysate(49.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

PPM1F monoclonal antibody (M01), clone 2A9 Western Blot analysis of PPM1F expression in Jurkat ( Cat # L017V1 ). Mouse IgG2a 2A9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Protein phosphatase 1F / PPM1F Mouse IgG2a 1G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PPM1F polyclonal antibody ( Cat # PAB2155 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PPM1F polyclonal antibody ( Cat # PAB2156 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F antibody

Immunohistochemistry-Paraffin: Protein phosphatase 1F Antibody [NBP1-88206] - Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Protein phosphatase 1F / PPM1F antibody

Immunohistochemistry-Paraffin: Protein phosphatase 1F Antibody [NBP1-88207] - Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in glomeruli. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Protein phosphatase 1F / PPM1F

Protein phosphatase 1F / PPM1F Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Protein phosphatase 1F / PPM1F (1-454, His-tag)

Protein phosphatase 1F / PPM1F Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Protein phosphatase 1F / PPM1F (1-454, His-tag)

Protein phosphatase 1F / PPM1F Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Protein phosphatase 1F / PPM1F

Protein phosphatase 1F / PPM1F Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Protein phosphatase 1F / PPM1F

Protein phosphatase 1F / PPM1F Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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