Prothymosin alpha antibody

Principal name

Prothymosin alpha antibody

Alternative names for Prothymosin alpha antibody

PTMA, TMSA, Thymosin alpha-1

SwissProt ID

P01252 (Bovin), P06302 (Rat), P06454 (Human), P26350 (Mouse)

Gene ID

5757 (PTMA)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Prothymosin alpha antibody

The thymus gland produces several hormones or hormone-like substances which are derived from a polypeptide precursor containing (in the rat) 113 amino acids and known as prothymosin alpha. A peptide containing 28 amino acid residues, named thymosin alpha 1, was originally isolated from calf thymosin fraction 5 and shown to restore various aspects of immune function in several in vitro and in vivo test systems. Thymosin alpha 1 was subsequently isolated from a similar fraction from human thymosin and reported to have the same amino acid sequence as bovine thymosin alpha 1.

General readings

Qi, X., et al. Biochemistry 49(9): 1923-1930 (2010)
Skopeliti, M., et al. Mol. Immunol. 46(5): 784-792 (2009)
Pawlikowski, M., et al. Folia Histochem. Cytobiol. 47(4): 559-562 (2009)
Zakharova, N.I., et al. Mol. Biol. (Mosk.) 42(4): 673-684 (2008)
Gudbjartsson, D.F., et al. Nat. Genet. 40(5): 609-615 (2008)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Prothymosin alpha antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human lymphoma and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lymphoma tissue using PTMA antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prothymosin alpha antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PTMA antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 8H12 was used at 1 µg/ml and the HRP conjugated goat anti-mouse IgG was used at 1:2,000 dilution. Mouse IgG2a 8H12 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PSMA was loaded at 200ng/lane. The anti-PTMA clone 9A10 was used at 1µg/ml and the HRP conjugated Goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG2a 9A10 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 6A3 was used at 1 µg/ml and the HRP conjugated goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG1 6A3 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 4G2 was used at 1 µg/ml and the HRP conjugated goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG1 4G2 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 6H7 was used at 1 µg/ml and the HRP conjugated goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG1 6H7 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 1F2 was used at 1 µg/ml and the HRP conjugated goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG1 1F2 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 4A7 was used at 1 µg/ml and the HRP conjugated goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG1 4A7 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 5A10 was used at 1 µg/ml and the HRP conjugated goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG2a 5A10 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

The GST tagged recombinant PTMA was loaded at 200 ng/lane. The anti-PTMA clone 4C3 was used at 1 µg/ml and the HRP conjugated goat anti-Mouse IgG was used at 1/2,000 dilution. Mouse IgG2a 4C3 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Prothymosin alpha (101-109) antibody

Figure 1. Immunofluorescence Image of Prothymosin alpha staining in Paraffin Sections of Rat Testis. The section was incubated with AP02197SU  at 1/1000 dilution followed by an appropriate secondary antibody coupled to Cy3. The nuclei were stained with DAPI. Original magnification: x40 Rabbit Serum Hu, Rt E, P
0.1 ml / €340.00
  Acris Antibodies GmbH

Prothymosin alpha (101-109) antibody

Figure 1. Immunofluorescence Image of Prothymosin alpha staining in Paraffin Sections of Rat Testis. The section was incubated with AP02197SU  at 1/1000 dilution followed by an appropriate secondary antibody coupled to Cy3. The nuclei were stained with DAPI. Original magnification: x40 Rabbit Serum Hu, Rt E, P
20 µl / €140.00
  Acris Antibodies GmbH

Prothymosin alpha (75-109) antibody

Figure 2. Immunohistochemistry of Prothymosin alpha staining in Paraffin sections of Rat testis. The sections were incubated with AP02198SU (1/1000)and detected using ABC method. The sections were counterstained with hematoxylin. 
Image A: AP02198SU stains Lydig cells (arrowhead), pachytene primary spermatozoides (arrow), and newly formed spermatids (asterisks). 
Image B: Control section without primary antibody. 
Scale Bar = 20 µm. 
Ferrara D et al. (2010) J. Cell. Physiol. 224(2):362-8. Rabbit Serum Hu, Rt E, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

Prothymosin alpha (75-109) antibody

Figure 2. Immunohistochemistry of Prothymosin alpha staining in Paraffin sections of Rat testis. The sections were incubated with AP02198SU (1/1000)and detected using ABC method. The sections were counterstained with hematoxylin. 
Image A: AP02198SU stains Lydig cells (arrowhead), pachytene primary spermatozoides (arrow), and newly formed spermatids (asterisks). 
Image B: Control section without primary antibody. 
Scale Bar = 20 µm. 
Ferrara D et al. (2010) J. Cell. Physiol. 224(2):362-8. Rabbit Serum Hu, Rt E, P, WB
20 µl / €170.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

Figure 1. Immunofluorescence of Thymosin alpha-1 staining of Paraffin Section of Human juvenile thymus. 
A: The section was incubated with AP02227SU (1/100) followed by an appropriate secondary antibody coupled to Cy3. The nuclei were stained with DAPI. Original magnification: x20. 
B: Control Section without primary antibody. Original magnification: x10. Rabbit Serum Hu, Ms C, E, P
0.1 ml / €440.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

Figure 1. Immunofluorescence of Thymosin alpha-1 staining of Paraffin Section of Human juvenile thymus. 
A: The section was incubated with AP02227SU (1/100) followed by an appropriate secondary antibody coupled to Cy3. The nuclei were stained with DAPI. Original magnification: x20. 
B: Control Section without primary antibody. Original magnification: x10. Rabbit Serum Hu, Ms C, E, P
20 µl / €170.00
  Acris Antibodies GmbH

Prothymosin alpha (N-term) antibody

Immunohistochemistry analysis in human uterus tissue (Formalin-fixed, Paraffin-embedded) using Prothymosin alpha antibody Cat.-No. AP53504PU-N (N-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of PTMA antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Prothymosin alpha antibody

Detection limit for recombinant GST tagged PTMA is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 1G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Prothymosin alpha antibody

Prothymosin alpha Mouse IgG2a 3E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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