PROX-1 antibody

Principal name

PROX-1 antibody

Alternative names for PROX-1 antibody

PROX1, Prospero homeobox protein 1, Homeobox prospero-like protein PROX1

SwissProt ID

P48437 (Mouse), Q91018 (Chick), Q92786 (Human)

Gene ID

5629 (PROX1), 19130 (Prox1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Fi (Fish), Chk (Chicken)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of PROX-1 antibody

The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.

General readings

1. Joshua D. Webster, Margaret A. Miller, Dee DuSold, and José Ramos-Vara. Effects of Prolonged Formalin-fixation on Diagnostic Immunohistochemistry in Domestic Animals.
J. Histochem. Cytochem., Apr 2009; 10.1369/jhc.2009.953877.
2. Ohad Shaham, April N. Smith, Michael L. Robinson, Makoto M. Taketo, Richard A. Lang, and Ruth Ashery-Padan. Pax6 is essential for lens fiber cell differentiation. Development, Aug 2009; 136: 2567-2578.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PROX-1 (C-term) antibody

DP3501 PROX-1 Antibody Staining of Paraffin
Embedded Human Mucosa Sections. Rabbit Serum Hu, Ms, Rt, Ze C, P, WB
0.2 ml / €370.00
  Acris Antibodies GmbH

PROX-1 (C-term) antibody

Immunofluorescence with a Frozen Section of a Mouse embryo at day 13.5 with anti-CD31 Antibody [Green] and anti-Human Prox-1 Antibody [red] (Cat.-No DP3516). It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1.  
The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany. Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
0.2 mg / €350.00
  Acris Antibodies GmbH

PROX-1 (C-term) antibody

Cross reaction of anti-Human Prox-1 with Horse tissue sections: Equine mandibular lymph node,paraffin section, stained for Prox1 antibody (Cat#DP3516P) counterstained with toluidine blue. Sinusoids (S) as well as lymphatic vessels in the hilum of the lymph node (arrows) are lined by positively stained lymphatic endothelial cells. Blood vascular endothelium remains unstained (arrow heads). LC: Cluster of lymphocytes.  From: Staszyk,C., K. F. Duesterdieck, H. Gasseu. A. Bienert, Immunohistochemical identification of lymphatic vessels in the periodontium of equine cheek teeth. J. Vet. Dent. 22,227–232,2005 Rabbit Aff - Purified Hu, Ms, Rt C, E, ICC/IF, P, WB
50 µg / €280.00
  Acris Antibodies GmbH

PROX-1 antibody

  Rabbit Polyclonal Antibody against PROX1 (Center)  
Western blot analysis of anti-PROX1 Antibody (Center) (Cat.#TA302031) in HepG2 cell line lysates (35ug/lane). PROX1(arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PROX-1 antibody

  mouse Anti-Prox1 Antibody  
Immunohistochemical staining of rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College. Mouse IgG 5G10 Aff - Purified Chk, Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PROX-1 (C-term) antibody

  Rabbit polyclonal PROX1 Antibody (C-term)  
Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

PROX-1 antibody

  Rabbit Polyclonal Anti-PROX1 Antibody  
Gel: 6%SDS-PAGE,Lysate: 40 ug,Lane 1-2: Human fetal liver tissue, 231 cells,Primary antibody: (PROX1 Antibody) at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PROX-1 (N-term) antibody

The anti-PROX1 N-term (AP11899PU-N) is used in Western blot to detect PROX1 in mouse brain tissue lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PROX-1 (723-727) antibody

Western blot analysis of extracts from A431 cells using Prospero homeobox protein 1 Antibody AP26049PU-N and the same antibody preincubated with blocking peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PROX-1 (723-727) antibody

Western blot analysis of extracts from A431 cells using Prospero homeobox protein 1 Antibody AP26049PU-N and the same antibody preincubated with blocking peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

PROX-1 (C-term) antibody

PROX-1 Rabbit Purified Fi, Hu, Ms, Rt C, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PROX-1 (C-term) antibody

PROX-1 Rabbit Biotin Hu, Ms, Rt ICC/IF
50 µg / €350.00
  Acris Antibodies GmbH

PROX-1 (C-term) antibody

Figure 1. Immunofluorescence with a Frozen Section of a Mouse embryo at day 13.5 with anti-CD31 [green] and anti-Human Prox-1 [red] (Cat.-No DP3516S). It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1 (Fig.1). The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany. Rabbit Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PROX1 - human gene knockout kit via CRISPR

PROX1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PROX1 - human gene knockout kit via CRISPR

PROX1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PROX1 - human gene knockout kit via CRISPR

PROX1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PROX1 - human gene knockout kit via CRISPR

PROX1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prox1 - mouse gene knockout kit via CRISPR

Prox1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Prox1 - mouse gene knockout kit via CRISPR

Prox1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Prox1 - mouse gene knockout kit via CRISPR

Prox1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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