PRPF19 / NMP200 antibody

Principal name

PRPF19 / NMP200 antibody

Alternative names for PRPF19 / NMP200 antibody

Pre-mRNA-processing factor 19,PRP19/PSO4 homolog, Nuclear matrix protein 200, Senescence evasion factor, PRP19, SNEV

SwissProt ID

Q08E38 (Bovin), Q5ZMA2 (Chick), Q99KP6 (Mouse), Q9JMJ4 (Rat), Q9UMS4 (Human)

Gene ID

27339 (PRPF19)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Dros (Drosophila)

Available hosts

Mouse, Rabbit, Guinea Pig

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Dot blot (Dot), Immunoprecipitation (IP)

Background of PRPF19 / NMP200 antibody

The human nuclear matrix proteins (hNMP) have reassembling and potential filament forming capabilities. The hNMP200 has a 56kDa nuclear protein that is also found in metazoans, plants, yeast. This subunit is homologous to saccharomycyes cerevisiae protein prp19 which play an essential role in pre-mRNA processing. The human homolog of rpr19 is involved in double DNA strand break repair. A number of proteins involved in adapter or regulatory modules in signal transduction, pre mRNA processing and cytoskeletal assembly typically contains a GH dipeptide 11-24 residues from its N-terminus and WD dipeptide at its C-terminus and is 40 amino acids long hence is called WD40 motif. A number of eukaryotic proteins contain WD40 domain that perform variety of funcvtions including signal transduction, pre mRNA processing and cytoskeletal assembly. The WD40 domains typically contain GH dipeptide 11-24 residues from its N-terminus and the WD didpeptide at its C-terminus an is generally 40 amino acids long, between GH and WD lies a conserved core that serves as a propeller like plateform to which protein can bind stably or reversibly. The core has propeller like structures with several blades where each blade is composed of 4 stranded anti-parallel beta sheet. The residues on the top and bottom surface of the propeller are proposed to coordinatre interactions with other proteins and /or small ligands. This protein motif is found in Prp19-like proteins, the regions represented by this family cover the sequences implicated in self-interaction and a coiled –coiled motif. Prp19-like proteins form an oligomer that is necessary for spliceosome assembly.
The formation of DNA interstrand cross-links (ICLs) are the most formidable lesion encountered by the cellular DNA repair machinery, and the elucidation of the process by which they are removed in eukaryotic cells was a daunting task for cell machinery to overcome. In particular, the early stages of adduct recognition and uncoupling of the cross-link have remained elusive. The ICL repair activity is contributed partly by mismatch repair factor MutSbeta, the nucleotide excision repair heterodimer Ercc1-Xpf and the replication protein RPA and PCNA are involved in the early stages of ICL repair mechanism. Two additional factors previously characterized are pre-mRNA splicing complex composed of PSO4/Prp19f, Cdc5L, Prlg1, and Spf27, also known as PSO4 complex and WRN, the protein deficient in Werners syndrome. Prp19f is located on chromosome 1 at location 1q43.
The Prp19 is a 400 amino acids protein in rat and the human homolog is 504 aa long due to extended N-terminal of the protein. A 346 amino acid core protein is homologous in both rat and human Prp19f. the Prp19f undergoes phosphorylation at serine-265 (serine 373 human), the kinase and implication of this phosphorylation is not yet fully understood.

General readings

Voglauer,R., (2006) Exp. Cell Res. 312 (6), 746-759

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PRP19P (C-term) antibody

PRP19P Guinea Pig Serum Hu, Ms C, ICC/IF, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

PRPF19 / NMP200 antibody

WB Suggested Anti-PRPF19 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysatePRPF19 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRPF19 / NMP200 antibody

WB Suggested Anti-PRPF19 Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PRPF19 / NMP200 Control Peptide

PRPF19 / NMP200
0.25 mg / €250.00
  Acris Antibodies GmbH

PRPF19 / NMP200 antibody

PRPF19 / NMP200 Rabbit Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PRPF19 / NMP200 control peptide

PRPF19 / NMP200 WB
0.1 mg / €270.00
  Acris Antibodies GmbH

PRPF19 / NMP200 pSer265 antibody

Western Blot using PRPF19 antibody (AP10277PU-N at 1/500). Rabbit IgG Aff - Purified Hu, Ms WB
0.1 mg / €490.00
  Acris Antibodies GmbH

PRPF19 / NMP200 Control Peptide

PRPF19 / NMP200
0.1 mg / €250.00
  Acris Antibodies GmbH

PRPF19 / NMP200 (263-276) antibody

Western Blot using PRPF19 Antibody (AP10284PU-N at 1/500) Rabbit IgG Aff - Purified Hu, Ms WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PRPF19 / NMP200 antibody

PRPF19 polyclonal antibody (A01), Lot # 051121JC01 Western Blot analysis of PRPF19 expression in HeLa ( Cat # L013V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PRPF19 / NMP200 antibody

PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG2a 2E5 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PRPF19 / NMP200 antibody

Western blot analysis of extracts from COLO/Jurkat cells, using PRPF19 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

PRPF19 / NMP200 antibody

Western blot analysis of extracts from COLO/Jurkat cells, using PRPF19 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

PRPF19 / NMP200 antibody

Human HepG2; WB Suggested Anti-PRPF19 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. PRPF19 is supported by BioGPS gene expression data to be expressed in HepG2.; PRPF19 antibody - N-terminal region (ARP43157_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

PRPF19 / NMP200 antibody

Human kidney; PRPF19 antibody - middle region (ARP43158_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

PRPF19 / NMP200 antibody

Immunohistochemical analysis of PRPF19 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PRPF19 / NMP200 antibody

Immunohistochemical analysis of PRPF19 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PRPF19 / NMP200 antibody

Immunohistochemical analysis of PRPF19 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

PRPF19 / NMP200 antibody

Immunohistochemistry: PRP19 Antibody [NBP1-31246] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PRP19 antibody at 1:500 dilution. Rabbit IgG Hu Dot, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

PRPF19 / NMP200 antibody

Immunohistochemistry: PRP19 Antibody [NBP1-31354] - Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NBP1-31354 at 1:100 dilution. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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