psbA antibody

Principal name

psbA antibody

Alternative names for psbA antibody

Photosystem Q(B) protein, 32 kDa thylakoid membrane protein, Photosystem II protein D1

SwissProt ID

P05337 (Horvu), P06585 (Pea), P12463 (Wheat), P48183 (Maize), P69556 (Tobac), P69560 (Spiol)

Gene ID

2715607 (psbA)

Available reactivities

Arabidopsis, Bac (Bacteria), Pl (Plant)

Available hosts

Rabbit, Chicken

Available applications

Western blot / Immunoblot (WB)

Background of psbA antibody

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

psbA antibody

psbA Chicken Purified Arabidopsis, Chlamydomonas, Cyanobacteria, higher Plants WB
0.1 ml / €400.00
  AgriSera AB

psbA antibody

psbA Chicken Purified Arabidopsis, Chlamydomonas, Cyanobacteria, higher Plants WB
10 µl / €160.00
  AgriSera AB

psbA (WB Standard)

psbA Lyophilized WB
1 Pack / €200.00
  AgriSera AB

psbA (C-term) antibody

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB, (3) Chlamydomonas reinhardtii total cell extracted with PEB, (4) Synechococcus sp. 7942 total cell extracted with PEB, (5) Anabaena sp. total cell extracted with PEB were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Rabbit Serum Algae, Arabidopsis, Bac, Pl WB
50 µl / €300.00
  AgriSera AB

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