psbC / CP43 antibody

Principal name

psbC / CP43 antibody

Alternative names for psbC / CP43 antibody

Photosystem II CP43 chlorophyll apoprotein, PSII 43 kDa protein, Photosystem II 44 kDa reaction center protein, Protein CP-43, Protein P6, AtCg00280

SwissProt ID

P06003 (Spiol), P06004 (Pea), P11095 (Horvu), P48187 (Maize), P56778 (Arath), Q2PMT9 (Soybn), Q9XPS4 (Wheat)

Gene ID

844773 (psbC)

Ncbi ID

NP_051055

Available reactivities

Arabidopsis, Pl (Plant)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB)

Background of psbC / CP43 antibody

PsbC (CP43) acts as an antenna to the PSII core and its presence seem to be also necessary for maintaining water splitting activity. This protein is more weakly associated with the PSII reaction centre and can be removed from the isolated core.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

psbC / CP43 antibody

5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB, (3) Chlamydomonas reinhardtii total cell extracted with PEB, (4) Synechococcus sp. 7942 total cell extracted with PEB,  extracted with PEB were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 75 seconds. Rabbit Serum Arabidopsis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Horerum vulgare, Oryza, Pl, Synechocystis sp. PCC6803, Vigna radiate. WB
50 µl / €300.00
  AgriSera AB

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