PSBP antibody

Principal name

PSBP antibody

Alternative names for PSBP antibody

Oxygen-evolving enhancer protein 2 chloroplastic, 23 kDa subunit of oxygen evolving system of photosystem II, 23 kDa thylakoid membrane protein, OEC 23 kDa subunit

SwissProt ID

P12302 (Spiol), P16059 (Pea), Q00434 (Wheat)

Ncbi ID

3562

Available reactivities

Arabidopsis

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSBP (23 kDa OEC) antibody

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300), (5) Anabaena sp. total cell extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Rabbit Purified Arabidopsis, Chlamydomonas reinhardti and Spinacia oleracea., Hordeum. Vulgare, Pinus banksiana WB
0.26 mg / €390.00
  AgriSera AB

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