PSMB10 antibody

Principal name

PSMB10 antibody

Alternative names for PSMB10 antibody

PSMB10, MECL1, Proteasome subunit beta type-10, EC=3.4.25.1, Proteasome subunit beta-2i, Proteasome MECl-1, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1

SwissProt ID

O35955 (Mouse), P40306 (Human), Q3T0T1 (Bovin)

Gene ID

5699 (PSMB10), 3569935 (IPR000438)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PSMB10 antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq]

General readings

Moschonas A, Kouraki M, Knox PG, Thymiakou E, Kardassis D, Eliopoulos AG. CD40 induces antigen transporter and immunoproteasome gene expression in carcinomas via the coordinated action of NF-kappaB and of NF-kappaB-mediated de novo synthesis of IRF-1. Molecular and Cellular Biology 2008 Oct 28 (20): 6208-22. PMID: 18694960

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMB10 antibody

TA305923 (0.3ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMB10 antibody

WB Suggested Anti-PSMB10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Hu, Ms, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMB10 (C-term) antibody

Western blot analysis of PSMB10 Antibody (C-term) Cat.-No AP53486PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PSMB10 antibody

Immunofluorescence of monoclonal antibody to PSMB10 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 3F8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMB10 antibody

Human Placenta; WB Suggested Anti-PSMB10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; PSMB10 antibody - middle region (ARP56474_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PSMB10 antibody

PSMB10 Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PSMB10 antibody

Western Blot: PSMB10 Antibody [NBP1-58937] - This Anti-PSMB10 antibody was used in Western Blot of Placenta tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

PSMB10 antibody

Immunohistochemistry-Paraffin: PSMB10 Antibody [NBP1-88660] - Immunohistochemical staining of human lymph node shows distinct cytoplasmic and nuclear positivity in lymphoid cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMB10 (40-273, His-tag)

Recombinant human PSMB10, 40-273aa, His-tagged Human Purified > 90 % E. coli
50 µg / €750.00
  Acris Antibodies GmbH

PSMB10 (40-273, His-tag)

Recombinant human PSMB10, 40-273aa, His-tagged Human Purified > 90 % E. coli
10 µg / €300.00
  Acris Antibodies GmbH

PSMB10

PSMB10 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PSMB10

PSMB10 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PSMB10 overexpression lysate

PSMB10 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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