PSMB9 antibody

Principal name

PSMB9 antibody

Alternative names for PSMB9 antibody

Proteasome subunit beta type-9, Proteasome 20S LMP2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta-1i, LMP2, PSMB6i, RING12, Multicatalytic endopeptidase complex chain 7, Really interesting new gene 12 protein

SwissProt ID

P28065 (Human), P28076 (Mouse), P28077 (Rat), Q3SZC2 (Bovin)

Gene ID

5698 (PSMB9), 905698 (acpS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Ze (Zebrafish), African clawed frog, Rb (Rabbit), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of PSMB9 antibody

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This subunit is involved in antigen processing to generate class I binding peptides. Expression of PSMB9 is induced by gamma interferon and it replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding different isoforms have been identified; both isoforms are processed to yield the same mature subunit.

General readings

General / background references:Voges D et al. The 26S proteasome: a molecular machine designed for controlled proteolysis. Annu Rev Biochem 68:1015-68 (1999). PubMed PMID: 10872471
Rechsteiner M Ubiquitin-mediated pathways for intracellular proteolysis. Annu Rev Cell Biol 3:1-30 (1987). PubMed PMID: 2825735

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMB9 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504348) Mouse IgG2b OTI1C4 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504349) Mouse IgG2a OTI1D1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504350) Mouse IgG1 OTI1G7 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504388) Mouse IgG1 OTI3H1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG1 OTI1F10 Purified Hu, Ms, Rt F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504390) Mouse IgG1 OTI3F3 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504391) Mouse IgG1 OTI2G6 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504392) Mouse IgG1 OTI2B6 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

AP22385PU-N PSMB9 antibody staining of Rat Thymus lysate at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PSMB9 antibody

Predicted band size: 23 kDa . Positive control: Raji, HepG2, THP-1 and Du145 cell, mouse liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Raji cell lysate Lane2: HepG2 cell lysate Lane3: THP-1 cell lysate Lane4: Du145 cell lysate Lane5: Mouse liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMB9 antibody

Western blot analysis of extracts of various cell lines, using PSMB9 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMB9 (C-term) antibody

Western blot analysis of PSMB9 Antibody (C-term) (AP18163PU-N) in HL-60 cell line lysates (35ug/lane). PSMB9 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PSMB9 antibody

PSMB9 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PSMB9 antibody

Human Jurkat; WB Suggested Anti-PSMB9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. PSMB9 is supported by BioGPS gene expression data to be expressed in Jurkat.; PSMB9 antibody - C-terminal region (ARP46081_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PSMB9 antibody

Western Blot: Proteasome 20S LMP2 Antibody [NB300-625] - Figure 1 shows a Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using NB300-625. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PSMB9 antibody

PSMB9 Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PSMB9 antibody

Western Blot: Proteasome 20S LMP2 Antibody [NBP1-58331] - This Anti-PSMB9 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMB9 (transcript variant 1)

PSMB9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PSMB9 (21-219, His-tag)

PSMB9 Human Purified > 90 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

PSMB9 (21-219, His-tag)

PSMB9 Human Purified > 90 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

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