PSMB9 antibody

Principal name

PSMB9 antibody

Alternative names for PSMB9 antibody

Proteasome subunit beta type-9, Proteasome 20S LMP2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta-1i, LMP2, PSMB6i, RING12, Multicatalytic endopeptidase complex chain 7, Really interesting new gene 12 protein

SwissProt ID

P28065 (Human), P28076 (Mouse), P28077 (Rat), Q3SZC2 (Bovin)

Gene ID

5698 (PSMB9), 16912 (Psmb9), 24967

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Rb (Rabbit), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PSMB9 antibody

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator.
LMP2 is a catalytic subunit of the 20S proteasome and, upon interferon gamma-induction, replaces the delta subunit. LMP2 alters the specificity of the 20S proteasome and is critical for the production of MHC class I ligands, production of T-lymphocytes, and is suggested to increase the efficiency of antigen presentation of the immune response.
Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).

General readings

Jia M., et al, (2010) Proteomics Clin Appl. 4(10-11):816-28.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI1C4 (formerly 1C4)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504348) Mouse IgG2b OTI1C4 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)  
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504349) Mouse IgG2a OTI1D1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504350) Mouse IgG1 OTI1G7 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI3H1 (formerly 3H1)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504388) Mouse IgG1 OTI3H1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG1 OTI1F10 Purified Hu, Ms, Rt F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI3F3 (formerly 3F3)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504390) Mouse IgG1 OTI3F3 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI2G6 (formerly 2G6)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504391) Mouse IgG1 OTI2G6 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

  PSMB9 (Proteasome 20S LMP2) mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PSMB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504392) Mouse IgG1 OTI2B6 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PSMB9 antibody

AP22385PU-N PSMB9 antibody staining of Rat Thymus lysate at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PSMB9 antibody

  Anti-PSMB9 Rabbit Polyclonal Antibody  
Predicted band size: 23 kDa . Positive control: Raji, HepG2, THP-1 and Du145 cell, mouse liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Raji cell lysate Lane2: HepG2 cell lysate Lane3: THP-1 cell lysate Lane4: Du145 cell lysate Lane5: Mouse liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMB9 antibody

  PSMB9 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PSMB9 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMB9 (C-term) antibody

Western blot analysis of PSMB9 Antibody (C-term) (AP18163PU-N) in HL-60 cell line lysates (35ug/lane). PSMB9 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PSMB9 - human gene knockout kit via CRISPR

PSMB9 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PSMB9 - human gene knockout kit via CRISPR

PSMB9 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PSMB9 - human gene knockout kit via CRISPR

PSMB9 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMB9 (transcript variant 1)

  Recombinant protein of human proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) (PSMB9), transcript variant 1  
PSMB9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PSMB9 (21-219, His-tag)

PSMB9 Human Purified > 90 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

PSMB9 (21-219, His-tag)

PSMB9 Human Purified > 90 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

PSMB9 overexpression lysate

PSMB9 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PSMB9 (mGFP-tagged) - Human proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) (PSMB9), 200 uL, >10^7 TU/mL

Lenti ORF particles, PSMB9 (mGFP-tagged) - Human proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) (PSMB9), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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