PSMD10 / Gankyrin antibody

Principal name

PSMD10 / Gankyrin antibody

Alternative names for PSMD10 / Gankyrin antibody

26S proteasome regulatory subunit p28, 26S proteasome non-ATPase regulatory subunit 10

SwissProt ID

O75832 (Human), Q9Z2X2 (Mouse), Q9Z2X3 (Rat)

Gene ID

5716 (PSMD10)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), Hst (Hamster), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of PSMD10 / Gankyrin antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20. [provided by RefSeq]

General readings

Hori T., et al. (1998) Gene. 216(1): 113-122.Kaneko T., et al. (2009) Cell. 137: 914-925.Wang J., et al. (2009) Cytogenet Genome Res. 125: 87-97.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMD10 / Gankyrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMD10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50) Mouse IgG1 OTI3F6 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PSMD10 / Gankyrin antibody

Immunoperoxidase of monoclonal antibody to PSMD10 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG1 4B5 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Detection of Gankyrin in HeLa lysate. ECL detection in 20 seconds. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD10 / Gankyrin antibody

Western blot analysis of extracts of SW620 cell lines, using PSMD10 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMD10 / Gankyrin antibody

WB Suggested Anti-PSMD10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: ACHN cell lysate Rabbit IgG Purified Can, Hu, Ms, Por, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD10 / Gankyrin antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD10 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : A549 cell lysate 
Lane 2. : PC3 cell lysate 
Lane 3. : K562 cell lysate 
Lane 4. : HepG2 cell lysate 
Lane 5. : HeLa cell lysate 
Lane 6. : Jurkat cell lysate Mouse IgG1 AT1F4 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PSMD10 / Gankyrin antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD10 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : A549 cell lysate 
Lane 2. : PC3 cell lysate 
Lane 3. : K562 cell lysate 
Lane 4. : HepG2 cell lysate 
Lane 5. : HeLa cell lysate 
Lane 6. : Jurkat cell lysate Mouse IgG1 AT1F4 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

PSMD10 / Gankyrin antibody

Immunofluorescence of purified MaxPab antibody to PSMD10 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Immunofluorescence of purified MaxPab antibody to PSMD10 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Western Blot analysis of PSMD10 expression in transfected 293T cell line by PSMD10 MaxPab rabbit polyclonal antibody.

Lane 1: PSMD10 transfected lysate(24.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Mouse IgG1 3F10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Mouse IgG1 1B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Western blot analysis of PSMD10 in HeLa lysate using with PSMD10 polyclonal antibody ( Cat# PAB12056 ). Rabbit Bov, Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Human ACHN; WB Suggested Anti-PSMD10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate; PSMD10 antibody - middle region (ARP56482_P050) in Human ACHN cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Rabbit Biotin Hu WB
0.1 ml / €360.00
  Novus Biologicals Inc.

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Rabbit DyLight488 Hu WB
0.1 ml / €390.00
  Novus Biologicals Inc.

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Rabbit HRP Hu WB
0.1 ml / €390.00
  Novus Biologicals Inc.

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt, Ze WB
25 µl / €160.00
  Novus Biologicals Inc.

PSMD10 / Gankyrin antibody

Immunohistochemistry-Paraffin: Gankyrin Antibody [NBP1-82443] - Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMD10 / Gankyrin (transcript variant 1)

PSMD10 / Gankyrin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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