PSMD10 / Gankyrin antibody

Principal name

PSMD10 / Gankyrin antibody

Alternative names for PSMD10 / Gankyrin antibody

26S proteasome regulatory subunit p28, 26S proteasome non-ATPase regulatory subunit 10

SwissProt ID

O75832 (Human), Q9Z2X2 (Mouse), Q9Z2X3 (Rat)

Gene ID

5716 (PSMD10), 53380 (Psmd10), 116722

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of PSMD10 / Gankyrin antibody

The 26S proteasome is a multicatalytic proteise complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

General readings

Hori T., et al. (1998) Gene. 216(1): 113-122.Kaneko T., et al. (2009) Cell. 137: 914-925.Wang J., et al. (2009) Cytogenet Genome Res. 125: 87-97.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMD10 / Gankyrin antibody

  anti-PSMD10 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMD10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500781, Dilution 1:50) Mouse IgG1 OTI3F6 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PSMD10 / Gankyrin antibody

Immunoperoxidase of monoclonal antibody to PSMD10 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG1 4B5 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

  Rabbit Polyclonal Antibody against Gankyrin  
Detection of Gankyrin in HeLa lysate. ECL detection in 20 seconds. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD10 / Gankyrin antibody

  PSMD10 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of SW620 cell lines, using PSMD10 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMD10 / Gankyrin antibody

  Rabbit polyclonal Anti-PSMD10 Antibody  
WB Suggested Anti-PSMD10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: ACHN cell lysate Rabbit IgG Purified Can, Hu, Ms, Por, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD10 / Gankyrin antibody

  Rabbit Polyclonal Anti-PSMD10 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (PSMD10 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD10 / Gankyrin antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD10 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : A549 cell lysate 
Lane 2. : PC3 cell lysate 
Lane 3. : K562 cell lysate 
Lane 4. : HepG2 cell lysate 
Lane 5. : HeLa cell lysate 
Lane 6. : Jurkat cell lysate Mouse IgG1 AT1F4 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PSMD10 / Gankyrin antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD10 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : A549 cell lysate 
Lane 2. : PC3 cell lysate 
Lane 3. : K562 cell lysate 
Lane 4. : HepG2 cell lysate 
Lane 5. : HeLa cell lysate 
Lane 6. : Jurkat cell lysate Mouse IgG1 AT1F4 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

PSMD10 / Gankyrin antibody

Immunofluorescence of purified MaxPab antibody to PSMD10 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Immunofluorescence of purified MaxPab antibody to PSMD10 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Western Blot analysis of PSMD10 expression in transfected 293T cell line by PSMD10 MaxPab rabbit polyclonal antibody.

Lane 1: PSMD10 transfected lysate(24.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Mouse IgG1 3F10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

PSMD10 / Gankyrin Mouse IgG1 1B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Western blot analysis of PSMD10 in HeLa lysate using with PSMD10 polyclonal antibody ( Cat# PAB12056 ). Rabbit Bov, Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

PSMD10 / Gankyrin antibody

Human ACHN; WB Suggested Anti-PSMD10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate; PSMD10 antibody - middle region (ARP56482_P050) in Human ACHN cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

PSMD10 - human gene knockout kit via CRISPR

PSMD10 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PSMD10 - human gene knockout kit via CRISPR

PSMD10 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PSMD10 - human gene knockout kit via CRISPR

PSMD10 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PSMD10 - human gene knockout kit via CRISPR

PSMD10 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Psmd10 - mouse gene knockout kit via CRISPR

Psmd10 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

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