PSMD3 antibody

Principal name

PSMD3 antibody

Alternative names for PSMD3 antibody

26S proteasome non-ATPase regulatory subunit 3, Proteasome 19S S3, 26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, Proteasome subunit p58

SwissProt ID

O43242 (Human), P14685 (Mouse), P25161 (Drome), Q04908 (Caeel), Q2KJ46 (Bovin)

Gene ID

5709 (PSMD3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F)

Background of PSMD3 antibody

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMD3 antibody

Anti-PSMD3 mouse monoclonal antibody (TA504303) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMD3(RC202307). Mouse IgG2a OTI2C2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMD3 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG2a OTI2A2 Purified Can, Hu, Mky, Ms, Rt F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PSMD3 antibody

Western blot analysis of extracts from K562 cells, using PSMD3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

Predicted band size: 61 kDa . Positive control: Hela and Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/300dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

Predicted band size: 61 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

Predicted band size: 61kDa. Positive control: 293T, Hela and Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lane3: Jurkat cell lysate Lysates: 50 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

Predicted band size: 61kDa. Positive control: NIH/3T3 cell and human fetal kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: NIH/3T3 cell lysate Lane2: Human fetal kidney tissue lysate Lysates: 50 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

WB Suggested Anti-PSMD3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysatePSMD3 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

WB Suggested Anti-PSMD3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

PSMD3 Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD3 antibody

Human HepG2; WB Suggested Anti-PSMD3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. PSMD3 is supported by BioGPS gene expression data to be expressed in HepG2.; PSMD3 antibody - N-terminal region (ARP56479_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PSMD3 (Center) antibody

Human THP-1; WB Suggested Anti-PSMD3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; PSMD3 antibody - middle region (ARP56480_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PSMD3 antibody

Western blot analysis of PSMD3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PSMD3 antibody

Western blot analysis of PSMD3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PSMD3 antibody

Western blot analysis of PSMD3 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

PSMD3 antibody

PSMD3 Rabbit Hu E, P, WB
50 µg / €440.00
  Novus Biologicals Inc.

PSMD3 antibody

Western Blot: Proteasome 19S S3 Antibody [NBP1-31530] - Western blot analysis of NBP1-31530 on 293T (lane A), A431 (lane B), and H1299 (lane C), with 7.5% SDS PAGE, diluted at 1:500. Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMD3

PSMD3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PSMD3

PSMD3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PSMD3

PSMD3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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