PSMD4 / MCB1 antibody

Principal name

PSMD4 / MCB1 antibody

Alternative names for PSMD4 / MCB1 antibody

26S proteasome non-ATPase regulatory subunit 4, Proteasome 19S S5A, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, Antisecretory factor 1, ASF, Multiubiquitin chain-binding protein

SwissProt ID

O35226 (Mouse), P55034 (Arath), P55036 (Human), Q58DA0 (Bovin)

Gene ID

5710 (PSMD4), 19185 (Psmd4)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), African clawed frog, Ze (Zebrafish), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of PSMD4 / MCB1 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD4 encodes one of the non-ATPase subunits of the 19S regulator lid. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Elangovan,M., (2007) Biochem. Biophys. Res. Commun. 364 (2), 226-230

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMD4 / MCB1 antibody

  Rabbit Polyclonal Anti-PSMD4 Antibody  
WB Suggested Anti-PSMD4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Raji cell lysatePSMD4 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD4 / MCB1 antibody

  Rabbit Polyclonal Anti-PSMD4 Antibody  
WB Suggested Anti-PSMD4 Antibody Titration: 0.3ug/ml; Positive Control: Raji cell lysatePSMD4 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMD4 / MCB1 antibody

  Rabbit Polyclonal Anti-PSMD4 Antibody - N-terminal region  
WB Suggested Anti-PSMD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD4 / MCB1 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PSMD4 / MCB1 antibody

PSMD4 / MCB1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PSMD4 / MCB1 antibody

Western Blot analysis of PSMD4 expression in transfected 293T cell line (H00005710-T01) by PSMD4 MaxPab polyclonal antibody.

Lane 1: PSMD4 transfected lysate(40.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PSMD4 / MCB1 antibody

PSMD4 / MCB1 Mouse IgG2a 3C9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD4 / MCB1 antibody

PSMD4 / MCB1 Rabbit Hu WB
0.1 ml / €640.00
  Abnova Taiwan Corp.

PSMD4 / MCB1 antibody

PSMD4 / MCB1 Rabbit Serum Hu WB
0.1 ml / €370.00
  AgriSera AB

PSMD4 / MCB1 antibody

Human Brain; . Rabbit Anti-PSMD4 Antibody. . Catalog Number:. ARP32341 . Paraffin Embedded Tissue: Human neural cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Por, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PSMD4 / MCB1 antibody

Human Raji; WB Suggested Anti-PSMD4 Antibody Titration: 0.3ug/ml. Positive Control: Raji cell lysate. PSMD4 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells.; PSMD4 antibody - N-terminal region (ARP32342_T100) in Human Raji cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

PSMD4 / MCB1 antibody

Human Jurkat; Host:Rabbit. Target Name:PSMD4. Sample Tissue:Jurkat. Antibody Dilution: 1.0ug/ml. PSMD4 is supported by BioGPS gene expression data to be expressed in Jurkat.; PSMD4 antibody - N-terminal region (ARP58060_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Psmd4 - mouse gene knockout kit via CRISPR

Psmd4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Psmd4 - mouse gene knockout kit via CRISPR

Psmd4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Psmd4 - mouse gene knockout kit via CRISPR

Psmd4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Psmd4 - mouse gene knockout kit via CRISPR

Psmd4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMD4 / MCB1

  Recombinant protein of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4)  
PSMD4 / MCB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PSMD4 / MCB1 (full length, N-term HIS tag)

  Recombinant protein of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
PSMD4 / MCB1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

PSMD4 / MCB1

PSMD4 / MCB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PSMD4 / MCB1

PSMD4 / MCB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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