PSMD9 antibody

Principal name

PSMD9 antibody

Alternative names for PSMD9 antibody

26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27

SwissProt ID

O00233 (Human), O94393 (Schpo), P40555 (Yeast), Q10920 (Caeel), Q3SZ19 (Bovin), Q9CR00 (Mouse), Q9WTV5 (Rat)

Gene ID

5715 (PSMD9)

Available reactivities

Xen (Xenopus), Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P)

Background of PSMD9 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

This product has been used in:Toyoshima H & Hunter T p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell 78:67-74 (1994). PubMed PMID: 8033213
Polyak K et al. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 78:59-66 (1994). PubMed PMID: 8033212

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMD9 antibody

Predicted band size: 25kDa. Positive control: A549 cell, human liver cancer and fetal kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1:A549 cell lysate Lane2: Human liver cancer tissue lysate Lane3: Human fetal kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD9 antibody

Predicted band size: 25kDa. Positive control: Jurkat, A549 cell and Human fetal kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: A549 cell lysate Lane3: Human fetal kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMD9 antibody

Western blot analysis of extracts of various cell lines, using PSMD9 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMD9 antibody

WB Suggested Anti-PSMD9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD9 antibody

WB Suggested Anti-PSMD9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate PSMD9 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSMD9 antibody

PSMD9 Mouse IgG1 p27(26S-104) Supernatant Xen ICC/IF, IP, WB
5 ml / €230.00
  Acris Antibodies GmbH

PSMD9 antibody

Immunofluorescence of purified MaxPab antibody to PSMD9 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

PSMD9 antibody

PSMD9 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PSMD9 antibody

PSMD9 MaxPab rabbit polyclonal antibody. Western Blot analysis of PSMD9 expression in mouse spleen. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PSMD9 antibody

PSMD9 monoclonal antibody (M01), clone 3A4 Western Blot analysis of PSMD9 expression in HepG2 ( Cat # L019V1 ). Mouse IgG1 3A4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PSMD9 antibody

PSMD9 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PSMD9 (C-term) antibody

PSMD9 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PSMD9 antibody

Western Blot: 26S proteasome subunit 9 Antibody [NB100-92276] - (WB) analysis of p27 (P191) antibody in extracts from HeLa cells. Rabbit IgG Aff - Purified Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

PSMD9 antibody

Western Blot: p27 KIP 1  Antibody [NB600-1442] - Western blot using affinity purified anti-p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight 800 conjugated Gt-a-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lanes M). Other detection systems will yield similar results. Rabbit IgG Serum Hu E, IP, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

PSMD9 antibody

Western Blot: p27 KIP 1 Antibody (3D8) [NBP1-47490] - Western blot analysis using CDKN1B mAb against CDKN1B-hIgGFc transfected HEK293 cell lysate. Mouse IgG1 3D8 Purified Hu E, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

PSMD9 antibody

PSMD9 Mouse IgG1 IMD-27 Aff - Purified Hu, Ms, Rt WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PSMD9 antibody

PSMD9 Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMD9

PSMD9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PSMD9 (1-223, His-tag)

PSMD9 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

PSMD9 (1-223, His-tag)

PSMD9 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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