PSME1 antibody

Principal name

PSME1 antibody

Alternative names for PSME1 antibody

PSME-1, IFI5111, Proteasome activator complex subunit 1, Proteasome activator 28 subunit alpha, PA28alpha, PA28a, 11S regulator complex subunit alpha, REG-alpha, IGUP I-5111, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein

SwissProt ID

P58238 (Macfa), P97371 (Mouse), Q06323 (Human), Q4U5R3 (Bovin), Q63797 (Rat), Q64L94 (Pig)

Gene ID

5720 (PSME1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of PSME1 antibody

The 26S proteasome is a multicatalytic proteise complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alterte regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.The 26S proteasome is a multicatalytic proteise complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alterte regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

General readings

1. Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E. Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28. Arthritis Rheum. 2006 Jul;54(7):2175-83.
PMID: 16802355

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSME1 antibody

  Rabbit anti-PSME1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PSME1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSME1 (alpha) antibody

Immunohistochemical detection of PA28a on 4% PFA fixed sections of Human thymus with AM26530AF-N Mouse IgG2a 1G11 Azide Free Hu, Ms, Rt IP, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

PSME1 (isoform 1) antibody

  Goat Polyclonal Antibody against PSME1  
TA303226 (0.3µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSME1 antibody

  Rabbit Polyclonal Anti-PSME1 Antibody - middle region  
WB Suggested Anti-PSME1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSME1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate
Lane 2. : LNCap cell lysate
Lane 3. : A549 cell lysate
Lane 4. : 293T cell lysate
Lane 5. : Jurkat cell lysate
Lane 6. : Raji cell lysate
Lane 7. : A431 cell lysate Mouse IgG2a AT12H3 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PSME1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate
Lane 2. : LNCap cell lysate
Lane 3. : A549 cell lysate
Lane 4. : 293T cell lysate
Lane 5. : Jurkat cell lysate
Lane 6. : Raji cell lysate
Lane 7. : A431 cell lysate Mouse IgG2a AT12H3 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PSME1 - human gene knockout kit via CRISPR

PSME1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSME1 (transcript variant 1)

  Recombinant protein of human proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) (PSME1), transcript variant 1  
PSME1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PSME1 (1-249, His-tag)

PSME1 Human Purified > 90 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

PSME1 (1-249, His-tag)

PSME1 Human Purified > 90 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

PSME1 overexpression lysate

PSME1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PSME1 (mGFP-tagged) - Human proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) (PSME1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PSME1 (mGFP-tagged) - Human proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) (PSME1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, PSME1 (Myc-DDK tagged) - Human proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) (PSME1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PSME1 (Myc-DDK tagged) - Human proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) (PSME1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

13 Item(s)

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