PSME3 antibody

Principal name

PSME3 antibody

Alternative names for PSME3 antibody

Proteasome activator complex subunit 3, 11S regulator complex subunit gamma, REG-gamma, Ki nuclear autoantigen, PA28g, PA28gamma, Activator of multicatalytic protease subunit 3, Proteasome activator 28 subunit gamma

SwissProt ID

P61289 (Human), P61289-2, P61290 (Mouse), P61291 (Pig), Q5F3J5 (Chick)

Gene ID

10197 (PSME3)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PSME3 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME3 is the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring.The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified.The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified.

General readings

Matsudaira R, et al. Relationships between autoantibody responses to deletion mutants of Ki antigen and clinical manifestations of lupus. J Rheumatol. 2003 Jun;30(6):1208-14. PMID: 12784391

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSME3 antibody

Sample (30 ug of whole cell lysate). A: A549. B: H1299. C: HCT116. D: MCF-7. 12% SDS PAGE. TA308562 diluted at 1:2000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSME3 antibody

Western blot analysis of extracts of various cell lines, using PSME3 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSME3 antibody

WB Suggested Anti-PSME3 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysate.PSME3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PSME3 antibody

WB Suggested Anti-PSME3 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate.PSME3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSME3 antibody

Western Blot analysis of PSME3 expression in transfected 293T cell line (H00010197-T01) by PSME3 MaxPab polyclonal antibody.

Lane 1: PSME3 transfected lysate(28.05 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PSME3 antibody

PSME3 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PSME3 antibody

Human Kidney; Rabbit Anti-PSME3 Antibody. Catalog Number: ARP46142. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PSME3 Antibody - N-terminal region (ARP46142_P050) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

PSME3 antibody

Human HepG2; WB Suggested Anti-PSME3 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. PSME3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; PSME3 antibody - C-terminal region (ARP46143_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

PSME3 antibody

Western Blot: PSME3 Antibody [NB100-60557] - NB100-60557 (0.1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PSME3 antibody

Immunohistochemistry-Paraffin: PSME3 Antibody [NBP1-33498] - IHC-Paraffin embedded analysis of CA922 Xenograft at 1:100 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

PSME3 antibody

Immunohistochemistry-Paraffin: PSME3 Antibody [NBP1-54587] - Human Kidney: epithelial cells of renal tubule (indicated with arrows). Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSME3 (transcript variant 1)

PSME3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PSME3 (1-254, His-tag)

PSME3 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

PSME3 (1-254, His-tag)

PSME3 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

PSME3

PSME3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PSME3

PSME3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PSME3 293T Cell Transient Overexpression Lysate(Denatured)

PSME3 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

PSME3 overexpression lysate

PSME3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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