PSMF1 / PI31 antibody

Principal name

PSMF1 / PI31 antibody

Alternative names for PSMF1 / PI31 antibody

Proteasome inhibitor PI31 subunit

SwissProt ID

Q3SX30 (Bovin), Q5XIU5 (Rat), Q8BHL8 (Mouse), Q92530 (Human)

Gene ID

9491 (PSMF1), 689852 (Psmf1), 228769

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PSMF1 / PI31 antibody

The 26S proteasome is a multicatalytic proteise complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Altertive transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008].

General readings

Tanahashi N. et al. (1999) Mol Biol Rep 26: 3-9.Seeger M. et al. (1997) J. Biol. Chem. 272: 8145-8148.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PSMF1 / PI31 (PI3 Isoform 1) (C-term) antibody

  Goat Polyclonal Antibody against PI31 / PSMF1 (Isoform 1)  
TA302572 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMF1 / PI31 antibody

  Rabbit polyclonal antibody to Proteasome inhibitor PI31 subunit (proteasome (prosome, macropain) inhibitor subunit 1 (PI31))  
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. TA308605 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PSMF1 / PI31 antibody

  Rabbit anti-PSMF1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PSMF1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PSMF1 / PI31 antibody

  PSMF1 mouse monoclonal antibody, clone OTI2F9 (formerly 2F9)  
Anti-PSMF1 mouse monoclonal antibody (TA505192) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMF1(RC215482). Mouse IgG1 OTI2F9 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PSMF1 / PI31 antibody

  PSMF1 mouse monoclonal antibody, clone OTI2D7 (formerly 2D7)  
Anti-PSMF1 mouse monoclonal antibody (TA505275) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMF1(RC215482). Mouse IgG1 OTI2D7 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

PSMF1 - human gene knockout kit via CRISPR

PSMF1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PSMF1 - human gene knockout kit via CRISPR

PSMF1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Psmf1 - mouse gene knockout kit via CRISPR

Psmf1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Psmf1 - mouse gene knockout kit via CRISPR

Psmf1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PSMF1 / PI31 (transcript variant 2)

  Recombinant protein of human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 2  
PSMF1 / PI31 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PSMF1 / PI31 (transcript variant 1)

  Recombinant protein of human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 1  
PSMF1 / PI31 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PSMF1 / PI31 (1-271, His-tag)

PSMF1 / PI31 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

PSMF1 / PI31 (1-271, His-tag)

PSMF1 / PI31 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

PSMF1 overexpression lysate

PSMF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

PSMF1 overexpression lysate

PSMF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PSMF1 (mGFP-tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PSMF1 (mGFP-tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, PSMF1 (mGFP-tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PSMF1 (mGFP-tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, PSMF1 (Myc-DDK tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PSMF1 (Myc-DDK tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Lenti ORF particles, PSMF1 (Myc-DDK tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PSMF1 (Myc-DDK tagged) - Human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Psmf1 (GFP-tagged ORF) - Rat similar to Proteasome inhibitor PI31 subunit (LOC689852), 200 uL, >10^7 TU/mL

Lenti ORF particles, Psmf1 (GFP-tagged ORF) - Rat similar to Proteasome inhibitor PI31 subunit (LOC689852), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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