PTEN antibody

Principal name

PTEN antibody

Alternative names for PTEN antibody

MMAC1, TEP1, Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Phosphatase and tensin homolog, Mutated in multiple advanced cancers 1

SwissProt ID

O08586 (Mouse), P60483 (Canfa), P60484 (Human), Q9PUT6 (Xenla)

Gene ID

5728 (PTEN), 5395 (PMS2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Chk (Chicken), Ze (Zebrafish), Mky (Monkey)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Dot blot (Dot)

Background of PTEN antibody

PTEN, (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), is a tumor suppressor implicated in a large number of human tumors. The PTEN phosphatase incorporates the catalytic motif (HCXXGXXRS/T) that is a signature of the protein tyrosine phosphatase family. Recombinant human PTEN is a dual phosphatase with ability to dephosphorylate both tyrosine and serine/threonine residues. PTEN functions primarily as a lipid phosphatase to regulate signal transduction pathways, with a primary target identified as phosphatidylinositol 3,4,5 trisphosphate. In addition, PTEN presents weak tyrosine phosphatase activity, which may downregulate signaling pathways involving focal adhesion kinase or Shc. PTEN negatively regulates activation of the serine/threonine kinase Akt/PKB by blocking its phosphorylation, thereby inhibiting the PI 3 kinase Akt signaling pathway, which is important for cell survival. In vivo, the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity. Mutations in PTEN are associated with several common cancers including prostate, brain and breast cancer, and with Cowden's disease, an autosomal dominant disorder conferring susceptibility to benign and malignant tumors. Germline mutations of PTEN are also linked Lhermitte-Duclos disease and Bannayan-Zonana syndrome. Mutations of PTEN occur in 60 to 80% of prostate cancers. PTEN is also essential for embryonic development.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PTEN antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN antibody. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PTEN antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN antibody. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PTEN (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTEN antibody (N-term) Cat.-No AP15248PU-N which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PTEN antibody

Immunohistochemistry-Paraffin: PTEN Antibody [NB100-2229] - Detection of Human PTEN by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PTEN used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

PTEN (C-term) antibody

Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 µg/ml in either the (A) absence or (B) presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PTEN (N-term) antibody

Western blot analysis of PTEN in P815 cell lysate with PTEN (NT) antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PTEN antibody

Western blot analysis of extracts from HeLa cells, treated with Vanadate, using PTEN (Ab-370) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PTEN antibody

Western blot analysis of extracts from 293 cells, using PTEN (Ab-380/382/383) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PTEN antibody

Predicted band size: 47 kDa. Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PTEN antibody

Western blot analysis of PTEN expression in Vanadate treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PTEN antibody

Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 1B8 Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

PTEN antibody

Formalin-Fixed, Paraffin-Embedded Human prostate stained with PTEN antibody Cat.-No. AM10137SU-N using peroxidase conjugate and AEC chromogen. Note nuclear staining of glandular epithelial cell. Mouse IgG2a 6H2.1 Supernatant Hu P
1 ml / €690.00
  Acris Antibodies GmbH

PTEN pSer370 antibody

PTEN Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PTEN pSer380 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTEN pSer380 antibody (#AP01671PU-N) Rabbit Aff - Purified Hu, Ms P
0.1 mg / €350.00
  Acris Antibodies GmbH

PTEN pSer380/pThr382/383 antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (pSer380/pThr382/pThr383) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PTEN pSer380/pThr382/383 antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (pSer380/pThr382/pThr383) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

PTEN antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN antibody. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PTEN antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN antibody. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PTEN antibody

Polyclonal antibody against pTEN used at 20 µg/ml. The PTEN status in these cell lines is as follows: Hec1A and KLE have wild-type PTEN, and AN3CA, MDA, and RL-95 have mutant PTEN (truncating mutations) 293T cells are supposed to have wild-type PTEN by sequence, but the levels are much lower than Hec1A or KLE. The lane marked 7 is 293T-PTEN is from 293T cells transfected with a FLAG-tagged PTEN. The FLAG-tagged proteins run slightly slower. Lane 1 - ANA3CA, lane 2 - Hec 1A, lane 3-KLE, lane 4 - MDA, lane 5-RL-95, lane 6 - 293T, lane 7 - 292T cells transfected with FLAG pTEN, lane 8 - negative control. Sheep IgG Purified Hu IP, WB
0.25 mg / €360.00
  Acris Antibodies GmbH

PTEN antibody

Immunohistochemistry (IHC) analyzes of PTEN antibody (Cat.-No.: AP06295PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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