PTENP1 antibody

Principal name

PTENP1 antibody

Alternative names for PTENP1 antibody

Phosphatase and tensin homolog 2, PTH2, PTEN-rs, PTEN2, psiPTEN, Phosphatase and tensin homolog pseudogene 1

SwissProt ID

O43460 (Human)

Ncbi ID

9606

Available reactivities

Hu (Human), Chimp (Chimpanzee), Macaque, Ms (Mouse), Op (Opossum), Rt (Rat), Can (Canine), Ze (Zebrafish), Bov (Bovine), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of PTENP1 antibody

This gene (PTENP1) is a highly homologous pseudogene of PTEN. PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

General readings

1. Phillips,R.J., Mestas,J., Gharaee-Kermani,M., Burdick,M.D., Sica,A., Belperio,J.A., Keane,M.P. and Strieter,R.M. (2005) Epidermal growth factor and hypoxia-induced expression of CXC chemokine receptor 4 on non-small cell lung cancer cells is regulated by the phosphatidylinositol 3-kinase/PTEN/AKT/mammalian target of rapamycin signaling pathway and activation of hypoxia inducible factor-1alpha. J. Biol. Chem. 280 (23), 22473-22481.
2. Baak,J.P., Van Diermen,B., Steinbakk,A., Janssen,E., Skaland,I., Mutter,G.L., Fiane,B. and Lovslett,K. (2005) Lack of PTEN expression in endometrial intraepithelial neoplasia is correlated with cancer progression. Hum. Pathol. 36 (5), 555-561.
3. Steck,P.A., Pershouse,M.A., Jasser,S.A., Yung,W.K., Lin,H., Ligon,A.H., Langford,L.A., Baumgard,M.L., Hattier,T., Davis,T., Frye,C., Hu,R., Swedlund,B., Teng,D.H. and Tavtigian,S.V. (1997) Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat. Genet. 15 (4), 356-362.
4. Li,J., Yen,C., Liaw,D., Podsypanina,K., Bose,S., Wang,S.I., Puc,J., Miliaresis,C., Rodgers,L., McCombie,R., Bigner,S.H., Giovanella,B.C., Ittmann,M., Tycko,B., Hibshoosh,H., Wigler,M.H. and Parsons,R. (1997) PTEN, a putative protein tyrosine phosphatase gene mutated in
human brain, breast, and prostate cancer. Science 275 (5308), 1943-1947.
5. Kim S.K., Su L.K., Oh Y., Kemp B.L., Hong W.K., Mao L. (1998) Alterations of PTEN/MMAC1, a candidate tumor suppressor gene, and its homologue, PTH2, in small cell lung cancer cell lines. Oncogene 16:89-93.

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PTENP1 antibody

WB using Anti-PTEN-P1 antibody shows detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (lane 4), HEK293 (lane 5) and MCF7 (lane 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. Primary antibody was used at 1:500. IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000. Rabbit IgG Aff - Purified Can, Chimp, green Pufferfish, Hu, Macaque, Ms, Rt, Ze E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PTENP1 antibody

WB using Anti-PTEN-P1 antibody shows detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (lane 4), HEK293 (lane 5) and MCF7 (lane 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. Primary antibody was used at 1:500. IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000. Rabbit IgG Aff - Purified Can, Chimp, green Pufferfish, Hu, Macaque, Ms, Rt, Ze E, WB
25 µl / €190.00
  Acris Antibodies GmbH

PTENP1 antibody

Western Blot analysis of PTENP1 expression in transfected 293T cell line (H00011191-T01) by PTENP1 MaxPab polyclonal antibody.

Lane 1: PTENP1 transfected lysate(40.59 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PTENP1 antibody

Western Blot analysis of PTENP1 expression in transfected 293T cell line (H00011191-T01) by PTENP1 MaxPab polyclonal antibody.

Lane 1: PTENP1 transfected lysate(40.59 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PTENP1 antibody

Western blot using PTENP1 polyclonal antibody ( Cat # PAB9923 ) shows detection of endogenous PTENP1  in whole cell lysates from human derived cell lines HeLa ( lane 4 ), HEK293 ( lane 5 ) and MCF-7 ( lane 6 ).
The band at ~55 kDa ( arrowhead ) corresponds to PTENP1.
Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide.
The identity of lower molecular weight bands in lane 4 is unknown.
Briefly, each lane contains approximately 35 µg of lysate.
Primary antibody was used at a 1 : 500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye800TM conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature ( 800 nm channel, green ).
Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Chimp, Hu, Macaque, Ms, Op, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

PTENP1 antibody

Western Blot: PTENP1 Antibody [NB110-86973] - Western blot using NB110-86973 shows detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (Lanes 4), HEK293 (Lanes 5) and MCF7 (Lanes 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4 degrees C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the OdysseyInfrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit Aff - Purified Hu E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

PTENP1 antibody

PTENP1 Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PTENP1

PTENP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PTENP1

PTENP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PTENP1 293T Cell Transient Overexpression Lysate(Denatured)

PTENP1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

10 Item(s)

per page
  • LinkedIn