PTGER3 antibody

Principal name

PTGER3 antibody

Alternative names for PTGER3 antibody

Prostaglandin E receptor 3, Prostanoid EP3 receptor, PGE2 receptor EP3 subtype, PGE2-R, PGE receptor EP3 subtype, EP3-III, EP3-II, EP3, Prostaglandin E2 receptor EP3 subtype

SwissProt ID

P30557 (Mouse), P34979 (Bovin), P34980 (Rat), P43115 (Human), P46069 (Rabit), P50131 (Pig), Q28550 (Sheep)

Gene ID

5733 (PTGER3)

Available reactivities

Hu (Human), Rb (Rabbit), Bov (Bovine), Rt (Rat), Ms (Mouse), Eq (Equine), Bt (Bat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP)

Background of PTGER3 antibody

Eicosanoids exert their cellular action through specific G-protein coupled receptors. Prostanoids are the products of cyclooxygenases action on C-20 unsaturated fatty acids that are released by action of phospholipase A2 form cell membranes in response to various stimuli. Prostagandin E2 is the major prostanoid produced as a consequence of inflammation. Various PGE2 mediate defects on smooth muscle, vascular smooth muscle, muscle tone, renal salt and water re-absorption and rennin release has been described. Rapid progress has been made in cloning of members of the family of eicosanoid receptors. Kidney is the major site of arachidonic acid release and its subsequent enzymatic conversion to bioactive prostanoids via cyclo-oxygenase metabolic pathway. PGE2 is the only prostanoid described for which there are four E-Prostanoid receptors (designated EP1, EP2, EP3 and EP4) which have been shown to mediate a variety of effects of prostaglandin E2 (PGE2) on kidney glomerular heamodynamics, tublar salt and water reabsorption and on renal vasculature (1). EP3 receptors cause smooth muscle contraction in chick ileum and inhibit acid secretion in rat gastric mucosa, are activated by both sulprostone and AY 23636, but are not antagonized by SC19220 (2). EP3 receptors are negatively coupled to adenylate cyclase and upon activation decrease the cellular levels of cAMP (3). The EP3 have multiple variants that are expressed in glomeruli, Tamm-Horsefall negative late distal convoluted tubules, connecting segments, cortical and medullary collecting ducts as well as in the media and endothelial cells of arteries and areterioles (2).
The EP3 receptor protein is approx. 55 kDa (405 amino acids) protein which has 7 transmembrane domains, characteristic of G-protein coupled receptors. The EP3 sequence analyses suggest the presence of 7 transmembrane domains, a characteristic feature of GPCR. The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which might be the site for the interaction of G-proteins. The short carboxy terminal is intracellular and has putative post-translational sites.

General readings

Sarrazin P, Hackett JA, Fortier I, Gallant MA, de Brum-Fernandes A.
Role of EP3 and EP4 prostaglandin receptors in reorganization of the cytoskeleton in mature human osteoclasts.
J Rheumatol. 2004 Aug;31(8):1598-606.
PMID: 15290741

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PTGER3 (Cytopl. Dom) antibody

Human, Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

PTGER3 (Isoform 1, 2, 3) antibody

TA303309 (1µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PTGER3 antibody

Western blot analysis of extracts from K562 cells, using PE2R3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PTGER3 antibody

Immunofluorescence analysis of LOVO cells, using PTGER3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

PTGER3 antibody

WB Suggested Anti-PTGER3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: COLO205 cell lysateThere is BioGPS gene expression data showing that PTGER3 is expressed in COLO205 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PTGER3 antibody

WB Suggested Anti-PTGER3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PTGER3 antibody

WB Suggested Anti-PTGER3 Antibody Titration: 2.4 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Eq, Hu, Rb WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PTGER3 antibody

WB Suggested Anti-PTGER3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Stomach Rabbit IgG Purified Eq, Hu, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

PTGER3 antibody

Anti-PTGER3 / EP3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bt, Hu, Rb
50 µg / €379.00
  OriGene Technologies, Inc.

PTGER3 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

PTGER3 antibody

Figure 1. Western blot analysis using EP3 antibody (AM05326PU-N) on procine brain lysate at 1 µg/ml. Mouse IgG2a 5F5 Purified Bov, Hu, Rt WB
0.1 mg / €400.00
  Acris Antibodies GmbH

PTGER3 antibody

Immunohistochemistry (IHC) analyzes of EP3 antibody (Cat.-No.: AP01424PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PTGER3 (3rd Cytopl. Dom.) antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Elephant, Eq, Hu, Mky, Ms, Por, Prim, Rb, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

PTGER3 control peptide

PTGER3
0.25 mg / €250.00
  Acris Antibodies GmbH

PTGER3 (C-term) antibody

Western blot with AP09993PU-N (1:500). Rabbit Aff - Purified Hu, Ms, Rt C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PTGER3 control peptide

PTGER3
0.25 mg / €250.00
  Acris Antibodies GmbH

PTGER3 (C-term) antibody

PTGER3 Rabbit Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PTGER3 antibody

Western Blot analysis of PTGER3 expression in transfected 293T cell line (H00005733-T02) by PTGER3 MaxPab polyclonal antibody.

Lane 1: PTGER3 transfected lysate(42.68 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), F, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PTGER3 antibody

Western blot analysis using PTGER3 monoclonal antibody, clone 5F5 ( Cat # MAB1978 ) on porcine brain lysate at 1µg/ml. Bov, Hu, Rt
0.1 mg / €470.00
  Abnova Taiwan Corp.

PTGER3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PE2R3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

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