PUF60 / SIAHBP1 antibody

Principal name

PUF60 / SIAHBP1 antibody

Alternative names for PUF60 / SIAHBP1 antibody

Siah-binding protein 1, Ro-binding protein 1, ROBPI, FIR, Poly(U)-binding-splicing factor PUF60, FBP-interacting repressor

SwissProt ID

Q2HJG2 (Bovin), Q3UEB3 (Mouse), Q9UHX1 (Human), Q9UHX1-2, Q9WV25 (Rat)

Gene ID

22827 (PUF60), 67959 (Puf60)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Por (Porcine), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PUF60 / SIAHBP1 antibody

PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

General readings

Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D. The FBP interacting repressor targets TFIIH to inhibit activated transcription. Mol Cell. 2000 Feb;5(2):331-41. PMID: 10882074

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PUF60 / SIAHBP1 antibody

  Rabbit Polyclonal Anti-PUF60 Antibody  
WB Suggested Anti-PUF60 Antibody Titration: 0.5 ug/ml Positive Control: Human 293T Cell Lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 (C-term) antibody

  Goat Polyclonal Antibody against SIAHBP1  
TA302836 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

  Rabbit Polyclonal antibody to PUF60 (poly-U binding splicing factor 60KDa)  
Sample(30 ug of whole cell lysate). A:Hep G2. B:MOLT4. C: Raji . 7.5% SDS PAGE. TA308645 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

  Rabbit Polyclonal Anti-PUF60 Antibody  
WB Suggested Anti-PUF60 Antibody; Titration: 1.25 ug/ml; Positive Control: 293T cells lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

  Rabbit Polyclonal Anti-PUF60 Antibody  
WB Suggested Anti-PUF60 Antibody; Titration: 0.5 ug/ml; Positive Control: Human 293T Cell Lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

PUF60 / SIAHBP1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PUF60 - human gene knockout kit via CRISPR

PUF60 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PUF60 - human gene knockout kit via CRISPR

PUF60 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PUF60 - human gene knockout kit via CRISPR

PUF60 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Puf60 - mouse gene knockout kit via CRISPR

Puf60 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Puf60 - mouse gene knockout kit via CRISPR

Puf60 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Puf60 - mouse gene knockout kit via CRISPR

Puf60 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Puf60 - mouse gene knockout kit via CRISPR

Puf60 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PUF60 / SIAHBP1 (transcript variant 2)

  Recombinant protein of human poly-U binding splicing factor 60KDa (PUF60), transcript variant 2  
PUF60 / SIAHBP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PUF60 / SIAHBP1 (transcript variant 1)

  Recombinant protein of human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1  
PUF60 / SIAHBP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PUF60 overexpression lysate

PUF60 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

PUF60 overexpression lysate

PUF60 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PUF60 (mGFP-tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PUF60 (mGFP-tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,190.00
  OriGene Technologies, Inc.

Lenti ORF particles, PUF60 (mGFP-tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PUF60 (mGFP-tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PUF60 (Myc-DDK tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PUF60 (Myc-DDK tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €1,070.00
  OriGene Technologies, Inc.

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