PUF60 / SIAHBP1 antibody

Principal name

PUF60 / SIAHBP1 antibody

Alternative names for PUF60 / SIAHBP1 antibody

Siah-binding protein 1, Ro-binding protein 1, ROBPI, FIR, Poly(U)-binding-splicing factor PUF60, FBP-interacting repressor

SwissProt ID

Q2HJG2 (Bovin), Q3UEB3 (Mouse), Q9UHX1 (Human), Q9UHX1-2, Q9WV25 (Rat)

Gene ID

22827 (PUF60), 1228270 (Q98D76)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of PUF60 / SIAHBP1 antibody

PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

General readings

Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D. The FBP interacting repressor targets TFIIH to inhibit activated transcription. Mol Cell. 2000 Feb;5(2):331-41. PMID: 10882074

  1. Quick-Filters:
  2. Highly Validated (2)

Items 1 to 20 of 28 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PUF60 / SIAHBP1 antibody

WB Suggested Anti-PUF60 Antibody Titration: 0.5 ug/ml Positive Control: Human 293T Cell Lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

Human Intestine; Rabbit Anti-PUF60 Antibody. Catalog Number: ARP40760. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PUF60 antibody - C-terminal region (ARP40760_P050) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PUF60 / SIAHBP1 (C-term) antibody

TA302836 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

Sample(30 ug of whole cell lysate). A:Hep G2. B:MOLT4. C: Raji . 7.5% SDS PAGE. TA308645 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

WB Suggested Anti-PUF60 Antibody; Titration: 1.25 ug/ml; Positive Control: 293T cells lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

WB Suggested Anti-PUF60 Antibody; Titration: 0.5 ug/ml; Positive Control: Human 293T Cell Lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1 antibody

PUF60 / SIAHBP1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

PUF60 / SIAHBP1 antibody

SIAHBP1 MaxPab polyclonal antibody. Western Blot analysis of SIAHBP1 expression in human pancreas. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PUF60 / SIAHBP1 antibody

PUF60 / SIAHBP1 Mouse IgG2a 3H1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PUF60 / SIAHBP1 antibody

PUF60 / SIAHBP1 Mouse IgG1 1C1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PUF60 / SIAHBP1 antibody

PUF60 polyclonal antibody ( Cat # PAB6268 ) staining ( 1 µg/ml ) of A-431 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PUF60 / SIAHBP1 antibody

Human Kidney; Rabbit Anti-PUF60 Antibody. Catalog Number: ARP40759. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PUF60 antibody - C-terminal region (ARP40759_T100) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

PUF60 / SIAHBP1 antibody

Human Intestine; Rabbit Anti-PUF60 Antibody Catalog Number: ARP40760 Paraffin Embedded Tissue: Human Intestine Cellular Data: Epithelial cells of intestinal villas Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X; PUF60 antibody - C-terminal region (ARP40760_T100) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

PUF60 / SIAHBP1 antibody

NB 100-1125 (1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PUF60 / SIAHBP1 antibody

Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.   Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes.  For blotting immunoprecipitated PUF60 were used.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit Aff - Purified Hu P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PUF60 / SIAHBP1 antibody

PUF60 / SIAHBP1 Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

PUF60 / SIAHBP1 antibody

PUF60 / SIAHBP1 Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PUF60 / SIAHBP1 (transcript variant 2)

PUF60 / SIAHBP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PUF60 / SIAHBP1 (transcript variant 1)

PUF60 / SIAHBP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PUF60 / SIAHBP1

PUF60 / SIAHBP1 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Items 1 to 20 of 28 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn