PUMA antibody

Principal name

PUMA antibody

Alternative names for PUMA antibody

BBC3, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis

SwissProt ID

Q80ZG6 (Rat), Q99ML1 (Mouse), Q9BXH1 (Human)

Gene ID

27113 (BBC3), 27133 (KCNH5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), Frozen Sections (C)

Background of PUMA antibody

PUMA (p53 upregulated modulator of apoptosis) belongs to pro-apoptotic members of Bcl2 family's BH3-only subgroup, and besides its most critical role as mediator of p53/TP53-dependent/-independent apoptosis, it acts as a regulator of ER stress-induced neurol apoptosis also. PUMA is expressed ubiquitously and predomitely localizes to mitochondria where it execute induction of cytochrome c release. PUMA mediates apoptosis via direct binding with anti-apoptotic members of the Bcl-2 family and is capable of interacting with MCL1, BCL2A1, BCL2 and BCL2L1/BCL-XL. PUMA binding to inhibitory members of Bcl-2 family (Bcl-2-like proteins) leads to Bax and/or Bak displacement mediated activation and formation of pore-like structures over mitochondrial membrane, which permeabilizes the outer mitochondrial membrane, resulting in mitochondrial dysfunction and caspase activation. PUMA is induced by p53, D damage, glucocorticoid treatment, deprivation of growth factors etc. and through ER stress in a DDIT3/CHOP-dependent manner. PUMA has been reported to involve in multiple physiological and pathological processes, including immune response, cancer, neurodegenerative diseases and bacterial as well as viral infections.

General readings

1. Qiu W, Leibowitz B, Zhang L, Yu J, Growth factors protect intestinal stem cells from radiation-induced apoptosis by suppressing PUMA through the PI3K/AKT/p53 axis.
Oncogene 2010 Mar 29 (11): 1622-32.
PMID: 19966853

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PUMA (BH3 Domain) antibody

Confocal immunofluorescent analysis of Puma Antibody (BH3 domain) Cat.-No AP11313PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu, Ms F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PUMA antibody

Immunohistochemistry: PUMA Antibody (EP512Y) [NB110-57443] - Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PUMA (C-term) (cat. # 110-57443). Rabbit IgG EP512Y Hu F, ICC/IF, IP, P, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

PUMA antibody

Detection of the beta isoform of PUMA in HL-60 whole cell lysate (4 ug/ml). ECL detection at a 30 minute exposure. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PUMA (C-term) antibody

Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with PUMA antibody at 2 ug/ml Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA (N-term) antibody

Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at 2 ug /ml. Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA antibody

(0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA (76-170) antibody

Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 ug/mL. Mouse IgG1 2A8F6 Aff - Purified Hu, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA (76-170) antibody

Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 ug/mL. Mouse IgG1 2A9G5 Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA (76-170) antibody

Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 ug/mL. Mouse IgG1 10C5G1 Aff - Purified Hu, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA (76-170) antibody

Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 ug/mL. Mouse IgG1 10D4G7 Aff - Purified Hu, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA antibody

Gel: 10%SDS-PAGE,Lysate: 40 µg,,Primary antibody:BBC3 Antibody at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PUMA (C-term) antibody

Western blot analysis of PUMA in K562 human cell lysates (Left) and mouse 3T3 cell lysates (Right) using the antibody Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA (N-term) antibody

Western blot analysis of PUMA expression in K562 cell lysates using 1:500 dilution of the antibody Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA antibody

Western Blot: PUMA Antibody [TA336553] - Detection of PUMA in HL-60 whole cell lysates using TA336553. Rabbit Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PUMA antibody

Western Blot: PUMA Antibody [TA336662] - WB detection of PUMA in transfected U20S cells using TA336662 at a dilution of 1:250. Please note that this antibody is expected to detect alpha as well as the beta forms of this target. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA antibody

Western blot analysis of BBC3(PUMA) expression in 293 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PUMA (N-term) antibody

Western blot analysis of PUMA expression in K562 cell lysates using 1:500 dilution of the antibody Rabbit IgG Aff - Purified Hu P, WB
25 µg / €230.00
  Acris Antibodies GmbH

PUMA (C-term) antibody

Western blot analysis of PUMA in K562 human cell lysates (Left) and mouse 3T3 cell lysates (Right) using the antibody Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
25 µg / €230.00
  Acris Antibodies GmbH

PUMA (C-term) antibody

Formalin-Fixed Paraffin-Embedded Small intestine stained with PUMA Antibody Cat.-No AP07278PU-N at 10 µg/ml after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PUMA (C-term) antibody

PUMA Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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