Pumilio 2 / PUM2 antibody

Principal name

Pumilio 2 / PUM2 antibody

Alternative names for Pumilio 2 / PUM2 antibody

Pumilio homolog 2, PUM-2, Pumilio-2, PUMH2, KIAA0235

SwissProt ID

Q80U58 (Mouse), Q8TB72 (Human)

Gene ID

23369 (PUM2), 3233691 (Q8J308), 80913 (Pum2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), African clawed frog, Chimp (Chimpanzee), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Pumilio 2 / PUM2 antibody

Pumilio 2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding mRNA targets. It supports proliferation and self-renewal of stem cells by regulating the translation of key transcripts. The Pumilio gene encodes proteins that are required for development of germ stem cells in one or both sexes. The Pumilio protein interacts with the human Nanos1 protein and this interaction may play a conserved role in germ cell development. Pumilio 2 is highly expressed in testis and ovary and at lower levels in brain, heart, kidney, liver, muscle, placenta, intestine and stomach. It is also expressed in stem cells, germ cells and in most fetal tissues.

General readings

Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Curr Biol. 2005 Jan 26;15(2):171-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Pumilio 2 / PUM2 antibody

TA303005 (1µg/ml) staining of Human Epstein-Barr virus immortilised lymphoblastoid  lysate (35µg protein in RIPA buffer) with (B) and without (A)  blocking with the immunizing peptide.  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Chimp, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Pumilio 2 / PUM2 antibody

WB Suggested Anti-PUM2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Pumilio 2 / PUM2 antibody

WB Suggested Anti-PUM2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Pumilio 2 / PUM2 antibody

Detection of human and mouse PUM 2 in whole cell lysates from HeLa, 293T and Mouse NIH3T3 cells with Rabbit anti PUM 2 (AP05992PU-N). Immunoprecipitation of PUM 2 (3mcg/mg lysate) from Whole cell lysates of HeLa cells and subsequently detected by Western blotting with Rabbit anti PUM 2 (AP05992PU-N) Rabbit IgG Purified Hu IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Pumilio 2 / PUM2 antibody

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Pumilio 2 / PUM2 antibody

Pumilio 2 / PUM2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Detection limit for recombinant GST tagged PUM2 is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 5B6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Detection limit for recombinant GST tagged PUM2 is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 1C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Detection limit for recombinant GST tagged PUM2 is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 7B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Detection limit for recombinant GST tagged PUM2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 7C7 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Pumilio 2 / PUM2 Mouse IgG1 7C7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Pumilio 2 / PUM2 Mouse IgG1 3E7 Purified Hu, Ms E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Pumilio 2 / PUM2 Mouse IgG2b 1E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Pumilio 2 / PUM2 Mouse IgG2b 3E6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Pumilio 2 / PUM2 Mouse IgG2a 2F10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Pumilio 2 / PUM2 Mouse IgG2a 4G12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with PUM2 polyclonal antibody ( Cat # PAB2096 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

PUM2 polyclonal antibody ( Cat # PAB6759 ) ( 1 µg/ml ) staining of human Epstein-Barr virus immortilised lymphoblastoid lysate ( 35 µg protein in RIPA buffer ) with ( A ) and without ( B ) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Pumilio 2 / PUM2 antibody

Transfected 293T; WB Suggested Anti-PUM2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; PUM2 antibody - N-terminal region (ARP40810_T100) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Pumilio 2 / PUM2 antibody

Human Liver; WB Suggested Anti-PUM2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; PUM2 antibody - N-terminal region (ARP40811_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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