Rab GDI alpha / GDI1 antibody
Principal name
Rab GDI alpha / GDI1 antibody
Alternative names for Rab GDI alpha / GDI1 antibody
Oligophrenin-2, Protein XAP-4, GDIL, OPHN2, RABGDIA, XAP4, Rab GDP dissociation inhibitor alpha, Guanosine diphosphate dissociation inhibitor 1
Ncbi ID
9606, NP_001484, NP_001484.1, NP_034403.1, NM_010273, NM_017088, NM_001493
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of Rab GDI alpha / GDI1 antibody
- Kit Component:
- - KN306393G1, Gdi1 gRNA vector 1 in pCas-Guide vector
- KN306393G2, Gdi1 gRNA vector 2 in pCas-Guide vector
- KN306393D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bachner D, Sedlacek Z., et al. (1995) Hum Mol Genet 4 (4): 701–8.Sedlacek Z, Konecki DS., et al. (1995) Mamm Genome 5 (10): 633–9.
GDI1 monoclonal antibody (M08), clone 1H3 Western Blot analysis of GDI1 expression in IMR-32 ( Cat # L008V1 ).
Western Blot analysis of GDI1 expression in transfected 293T cell line by GDI1 monoclonal antibody (M08), clone 1H3.Lane 1: GDI1 transfected lysate(50.6 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged GDI1 is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-GDI1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; GDI1 antibody - C-terminal region (AVARP13086_T100) in Human HepG2 cells using Western Blot
Human , Mouse; GDI1 antibody - C-terminal region (AVARP13086_T100) in Human , Mouse cells using Western Blot
Western Blot analysis of GDI1 expression in transfected 293T cell line by GDI1 monoclonal antibody (M12), clone 2C2.Lane 1: GDI1 transfected lysate(50.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GDI1 expression in transfected 293T cell line (H00002664-T01) by GDI1 MaxPab polyclonal antibody.Lane 1: GDI1 transfected lysate(50.60 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of GDI1 expression in HepG2 (A); U87MG (B); SHSY5Y (C) whole cell lysates.
Predicted band size: 51kDa. Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/300 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/25-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using GDI1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x 200)
Predicted band size: 51kDa. Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 3 seconds)
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/ 25-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using GDI1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)
Human , Mouse
WB Suggested Anti-GDI1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Western blot analysis of extracts of various cell lines, using GDI1 antibody.
Immunohistochemical of paraffin-embedded human breast cancer using GDI1 antibody at dilution of 1:200 (400x lens).
Immunohistochemical of paraffin-embedded human stomach cancer using GDI1 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of MCF-7 cell using GDI1 antibody. Blue: DAPI for nuclear staining.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332701 | Rab GDI alpha / GDI1 antibody |
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| Rabbit anti-GDI1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA323161 | Rab GDI alpha / GDI1 antibody |
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| Anti-GDI1 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA323162 | Rab GDI alpha / GDI1 antibody |
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| Anti-GDI1 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA330010 | Rab GDI alpha / GDI1 antibody |
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| Rabbit Polyclonal Anti-GDI1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203824 | GDI1 - human gene knockout kit via CRISPR |
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 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203824BN | GDI1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203824LP | GDI1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203824RB | GDI1 - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN306393 | Gdi1 - mouse gene knockout kit via CRISPR |
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|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN306393LP | Gdi1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN306393RB | Gdi1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303824 | Rab GDI alpha / GDI1 |
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| Recombinant protein of human GDP dissociation inhibitor 1 (GDI1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR51509PU-N | Rab GDI alpha / GDI1 (1-447, His-tag) |
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 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.25 mg /
€940.00
|
| Acris Antibodies GmbH | |||||
| AR51509PU-S | Rab GDI alpha / GDI1 (1-447, His-tag) |
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|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
50 µg /
€370.00
|
| Acris Antibodies GmbH | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC203824L2V | Lenti ORF particles, GDI1 (mGFP-tagged) - Human GDP dissociation inhibitor 1 (GDI1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC203824L1V | Lenti ORF particles, GDI1 (Myc-DDK tagged) - Human GDP dissociation inhibitor 1 (GDI1) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207129L2V | Lenti ORF particles, Gdi1 (GFP-tagged) - Mouse guanosine diphosphate (GDP) dissociation inhibitor 1 (Gdi1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| MR207129L1V | Lenti ORF particles, Gdi1 (Myc-DDK-tagged) - Mouse guanosine diphosphate (GDP) dissociation inhibitor 1 (Gdi1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209998L2V | Lenti ORF particles, Gdi1 (GFP-tagged ORF) - Rat GDP dissociation inhibitor 1 (Gdi1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RR209998L1V | Lenti ORF particles, Gdi1 (Myc-DDK-tagged ORF) - Rat GDP dissociation inhibitor 1 (Gdi1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€967.00
|
|
| OriGene Technologies, Inc. | ||
